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Assay Kits
Assay Kit
CFSE Cell Division Tracker Kit
CFSE Cell Division Tracker Kit from BioLegend
(1)
Write a review
Citations:
(133)
Supplier Page
Supplier Page from
BioLegend for
CFSE Cell Division Tracker Kit
Description
CFSE Cell Division Tracker Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CFSE Cell Division Tracker Kit
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
423801
Quantity
1 kit
Applications
Intracellular Staining for Flow Cytometry (ICFC), in-vivo Cell Tracking
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (133)
(81)
Melatonin exerts immunoregulatory effects by balancing peripheral effector and regulatory T helper cells in myasthenia gravis
Aging
November 2, 2020
Chang T, Niu C, Sun C, Ma Y, Guo R, Ruan Z, Gao Y, Lu X, Li H, Lin Y,
et al
.
PBMCs using a CD4+
T Cell
Isolation Kit (130096553, Miltenyi Biotec) and incubated with 5 μM
CFSE
(
423801
,
BioLegend
) in sterile PBS for 10 minutes at 37°C in the dark. The
CFSE
-labeled CD4+ T cells were washed
(82)
Manganese is critical for antitumor immune responses via cGAS-STING and improves the efficacy of clinical immunotherapy
Cell research
November 1, 2020
Lv M, Chen M, Zhang R, Zhang W, Wang C, Zhang Y, Wei X, Guan Y, Liu J, Feng K,
et al
.
from Sigma-Aldrich (St. Louis, MO), unless otherwise stated.
CFSE
cell division tracer kit (
Biolegend
,
423801
),
Perm/Wash buffer
(
BD Biosciences
, 51-2091KZ), Red Blood Cell Lysis Buffer (Solarbio, R1010), 4% fixative
(83)
Lipid accumulation in macrophages confers protumorigenic polarization and immunity in gastric cancer
Cancer science
November 1, 2020
Luo Q, Zheng N, Jiang L, Wang T, Zhang P, Liu Y, Zheng P, Wang W, Xie G, Chen L,
et al
.
then purified CD8+ T cells were labeled with 5 µmol/L carboxyfluorescein succinimidyl ester (
CFSE
) (#
423801
,
BioLegend
) in PBS for 6 min at 37°C. The
CFSE
‐labeled CD8+ T cells were then plated in complete RPMI
(84)
Chemerin Reactivates PTEN and Suppresses PD-L1 in Tumor Cells via Modulation of a Novel CMKLR1-mediated Signaling Cascade
Clinical cancer research : an official journal of the American Association for Cancer Research
September 15, 2020
Rennier K, Shin WJ, Krug E, Virdi G, Pachynski RK.
(85)
Elevated Glucose Levels Favor SARS-CoV-2 Infection and Monocyte Response through a HIF-1α/Glycolysis-Dependent Axis
Cell metabolism
September 1, 2020
Codo AC, Davanzo GG, Monteiro LB, de Souza GF, Muraro SP, Virgilio-da-Silva JV, Prodonoff JS, Carregari VC, de Biagi Junior CAO, Crunfli F,
et al
.
(500μM),Sigma-Aldrich,Cat.G0750 carboxyfluorescein succinimidyl ester (CellTrace
CFSE
),
BioLegend
,Cat#
423801
,, Critical Commercial
Assays
,Critical Commercial
Assays
,Critical Commercial
Assays
,,
GoScript Reverse Transcriptase
(86)
Dose and duration of interferon γ pre-licensing interact with donor characteristics to influence the expression and function of indoleamine-2,3-dioxygenase in mesenchymal stromal cells
Journal of the Royal Society, Interface
June 1, 2020
Boyt DT, Boland LK, Burand AJ Jr, Brown AJ, Ankrum JA.
(87)
Suppression of Inflammasome Activation by IRF8 and IRF4 in cDCs Is Critical for T Cell Priming
Cell reports
May 5, 2020
McDaniel MM, Kottyan LC, Singh H, Pasare C.
Ovalbumin,
Invivogen
,
Vac-pova
Gentamycin Reagent,GIBCO,15750-060 OPD,
Sigma
,
P1526
CellTrace
Violet
,
Thermo Fisher
,
C34571
CFSE
,
Biolegend
,
423801
GM-CSF
,
Biolegend
,
576306
Lipofectamine 2000,Invitrogen,116680 Polybrene,Sigma,TR-1003 Critical Commercial
(88)
BCR-Induced Ca
2+
Signals Dynamically Tune Survival, Metabolic Reprogramming, and Proliferation of Naive B Cells
Cell reports
April 14, 2020
Berry CT, Liu X, Myles A, Nandi S, Chen YH, Hershberg U, Brodsky IE, Cancro MP, Lengner CJ, May MJ,
et al
.
,
Thermo Fisher
,Cat#
62–6520
RRID:AB_88369 ,, "Chemicals, Peptides, and Recombinant Proteins",, ,,
CFSE
,
Biolegend
,Cat#
423801
Fura-2AM,Molecular Probes,CAS 108964–32-5 FK506 (tacrolimus),
Tocris Bioscience
,Cat#
3631/10
Cyclosporine
(89)
Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway
Science advances
April 1, 2020
Lim J, Heo J, Ju H, Shin JW, Kim Y, Lee S, Yu HY, Ryu CM, Yun H, Song S,
et al
.
37°C in 5% CO2. Cell proliferation activity was analyzed using the
CFSE Cell Division Tracker Kit
(#
423801
;
BioLegend
, San Diego, CA, USA), and the percentage of CD3+ T cells (#
300412
; BD Biosciences) was analyzed
(90)
Aggregation of Human Mesenchymal Stromal Cells Eliminates Their Ability to Suppress Human T Cells
Frontiers in immunology
January 1, 2020
Burand AJ Jr, Di L, Boland LK, Boyt DT, Schrodt MV, Santillan DA, Ankrum JA.
T cells
, as described in the Supplemental Materials and Methods, were stained with CSFE (
Biolegend
, Cat #
423801
) as described by the manufacturer. Briefly, PBMCs or
T cells
were thawed and placed in warm complete
(91)
CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model
Frontiers in immunology
January 1, 2020
Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, Li J, Liang S, Xiong J, Li Z,
et al
.
μM carboxyfluorescein diacetate succinimidyl ester (
CFSE
) according to the manufacturer's protocol (
423801
,
Biolegend
, San Diego, CA, USA). Then, the iTregs were washed three times with RPMI 1640 medium and
(92)
Targeting EZH2 Enhances Antigen Presentation, Antitumor Immunity, and Circumvents Anti-PD-1 Resistance in Head and Neck Cancer
Clinical cancer research : an official journal of the American Association for Cancer Research
January 1, 2020
Zhou L, Mudianto T, Ma X, Riley R, Uppaluri R.
CD8+ T cells isolated from OT-1
mouse
splenocytes were stained with
CFSE cell division tracker kit
(
423801
,
BioLegend
) at a final concentration of 5 μM in PBS supplemented with 5% heat inactivated FBS for 3
(93)
Nanomicelle protects the immune activation effects of Paclitaxel and sensitizes tumors to anti-PD-1 Immunotherapy
Theranostics
January 1, 2020
Yang Q, Shi G, Chen X, Lin Y, Cheng L, Jiang Q, Yan X, Jiang M, Li Y, Zhang H,
et al
.
Miltenyi Biotech
Inc.). BMDCs and
macrophages
were stained with the
CFSE Cell Division Tracker Kit
(
423801
,
BioLegend
). CT26-RFP cells were treated with drugs as indicated for 4 h and then added to the
CFSE
-labeled
(94)
Age-Related Dopaminergic Innervation Augments T Helper 2-Type Allergic Inflammation in the Postnatal Lung
Immunity
December 17, 2019
Wang W, Cohen JA, Wallrapp A, Trieu KG, Barrios J, Shao F, Krishnamoorthy N, Kuchroo VK, Jones MR, Fine A,
et al
.
proliferation assay. Cell proliferation was analyzed with
CFSE Cell Division Tracker Kit
(
BioLegend
,
423801
) following the manufacturer’s protocol. Briefly, freshly isolated
lymphocytes
were incubated with 5
(95)
Signal Integration and Transcriptional Regulation of the Inflammatory Response Mediated by the GM-/M-CSF Signaling Axis in Human Monocytes
Cell reports
October 22, 2019
Rodriguez RM, Suarez-Alvarez B, Lavín JL, Ascensión AM, Gonzalez M, Lozano JJ, Raneros AB, Bulnes PD, Vidal-Castiñeira JR, Huidobro C,
et al
.
(96)
Measuring mitochondrial respiration in adherent cells infected with Trypanosoma cruzi Chagas, 1909 using Seahorse extracellular flux analyser
Folia Parasitol (Praha)
October 10, 2019
Laura Maria Gonzalez-Ortiz, Juana Patricia Sanchez-Villamil, Mike A. Celis-Rodriguez, Giovanni Lineros, Sandra Sanabria-Barrera, Norma C. Serrano, Melvin Y. Rincon, Paula K. Bautista-Nino
#
13995
by
Cayman Chemical Company
. • Carboxyfluorescein diacetate succinimidyl ester (
CFSE
), CAT #
423801
by
Biolegend
, (San Diego, CA, USA). *Oligomycin, FCCP, antimycin A and rotenone were initially reconstituted
(97)
Short-Acting Anti-VWF (von Willebrand Factor) Aptamer Improves the Recovery, Survival, and Hemostatic Functions of Refrigerated Platelets
Arteriosclerosis, thrombosis, and vascular biology
October 1, 2019
Chen 陈温纯 W, Voos KM, Josephson CD, Li R.
(APC)-labeled anti-mouse CD41 antibody (Cat:
133913
), and carboxyfluorescein succinimidyl ester (
CFSE
, Cat:
423801
) were purchased from
Biolegend
(San Diego, CA).
APC anti-human CD41
antibody (
clone
HIP8, Cat:
559777
)
(98)
Arming Tumor-Associated Macrophages to Reverse Epithelial Cancer Progression
Cancer research
October 1, 2019
Wettersten HI, Weis SM, Pathria P, Von Schalscha T, Minami T, Varner JA, Cheresh DA.
cellular-cytotoxicity (
ADCC) assay
Target cells stained with
CFSE Cell Division Tracker Kit
(
BioLegend
,
423801
) were co-cultured with effector cells with or without isotype IgG or LM609 for 5–16 hr at 37°C, stained
(99)
NLRP3 inflammasome in fibroblasts links tissue damage with inflammation in breast cancer progression and metastasis
Nature communications
September 26, 2019
Ershaid N, Sharon Y, Doron H, Raz Y, Shani O, Cohen N, Monteran L, Leider-Trejo L, Ben-Shmuel A, Yassin M,
et al
.
assay
Splenocytes were isolated from the
spleen
of Il1b−/−
mice
, and labelled with
CFSE
(5 M,
Biolegend
,
423801
). 1 × 105 cells in 100 μL per well were plated in a 96-well plate pre-coated with anti-
mouse
CD3ε (1
(100)
Treating ischemia via recruitment of antigen-specific T cells
Science advances
July 1, 2019
Kwee BJ, Seo BR, Najibi AJ, Li AW, Shih TY, White D, Mooney DJ.
cells were labeled with carboxyfluorescein diacetate succinimidyl ester (
CFSE
) (
BioLegend
, catalog no.
423801
) to track cell proliferation and were cultured with the
OVA
-pulsed BMDCs at an 8:1 (
T-cell
:
dendritic cell
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Reviews
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9/22/2017
CFSE Staining Kit for PBMC staining
Max Hübner
To assay long-term T-cell proliferation intensity correlating with immunoparalysis before and after surgical intervention we took advantage of this easy-to-handle assay.
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CFSE Cell Division Tracker Kit from BioLegend
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View Company Product Page
Pricing Info
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