Zombie Red™ Fixable Viability Kit 423110 from BioLegend

Product Specs
ItemZombie Red™ Fixable Viability Kit
CompanyBioLegend
Price $335.00Supplier Page View Company Product Page
Catalog Number423110
Quantity500 tests
ApplicationsFC - Quality tested, ICFC, ICC - Verified

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Krüppel-like factors 2 and 3 regulate T cell exhaustion by directing T cell residency and migrationImmunityMarch 27, 2026Jian Shen, Ryan J. Brown, Yuqi Zhang, Kexin Gai, Ashley K. Brown, Ashley M. Bauer, Ashley Zhu, Siying Lin, Hongshen Niu, Arjun Kharel, et al.Violet Stain Kit,Thermo Fisher Scientific,Cat# L34955 Zombie Red Fixable Viability Kit,BioLegend,Cat# 423110 Critical Commercial Assays,, EasySep Mouse Naïve CD8+ T Cell Isolation Kit,StemCell Technologies,Cat#

(2) Comprehensive single-cell metabolic profiling identifies targets to sensitize triple-negative breast cancer to chemo-immunotherapyCell Rep MedMarch 17, 2026Ying Xu, Xin-Yi Liu, Hang Zhang, Li Chen, Han Wang, Zhi-Ming Shao, Yi Xiao, Yi-Zhou Jianganti-mouse CD16/32 (clone 93),BioLegend,Cat# 101320; RRID: AB_1574975 FC: Zombie-RED,BioLegend,Cat# 423110 FC: APC/FIRE 750 anti-mouse CD45 (clone I3/2.3),BioLegend,Cat# 147714; RRID: AB_2750441 FC: PerCP/Cyanine5.5

(3) TBKBP1 induces capecitabine resistance through negative regulation of type I interferon pathway in triple-negative breast cancerOncogeneJanuary 28, 2026Wen-Ya Wu, Yun-Song Yang, Lisa Andriani, Yi-Fan Xie, Gen-Hong Di, Zhi-Ming Shao, Jun-Jie Li4 °C for 30 min. All the antibodies and reagents used for flow cytometry included Zombie-RED (ECD, #423110), CD45 (APC/Cy7, clone 30-F11, #103116), CD3e (PC5.5, clone 145-2C11, #100328), CD4 (AF700, clone RM4-5,

(4) Restoring immune homeostasis in atherosclerotic plaques via inorganic violet phosphorus nano-immunotherapyCell Reports MedicineJanuary 20, 2026Wanqin Zeng, Yingjun Fan, Chaohua Wang, Shuping Yang, Junchao Xu, Caijin Wang, Hucheng Chang, Yaoyao Luo, Shengbin Liu, Zhongshan Hecounted, diluted to 1 × 106 cells per 100 μL, and incubated with the Zombie Red Fixable Viability Kit (423110, BioLegend) in the dark at room temperature for 15 min. Cells were then washed twice with FACS buffer

(5) Macrophage repolarization by immune checkpoint blockade drives T cell engagement in the tumor microenvironmentiScienceOctober 17, 2025Tina Kwok, Ildefonso A. Silva-Junior, Sara Korpe, Haidong Dong, Jessica N. LancasterScientific,Cat#10030290 Matigel Matrix,Corning,Cat#CB40234A LPS from E.coli,Sigma Aldrich,Cat#L4391 Zombie Red,Biolegend,Cat#423110 GhostDye Red 780,Tonbo Bioscience,Cat#13-0865-T100 ,, Critical commercial assays,Critical commercial

(6) Single-Cell RNA Sequencing Integrated with Bulk-RNA Sequencing Analysis Reveals Prognostic Signatures Based on PANoptosis in Hepatocellular CarcinomaJ Hepatocell CarcinomaJuly 29, 2025Jiyin Wang, Xue Yin, Ziyi Li, Pu Liang, Yipeng Wang, Xingling Li, Wenying Qiao, Chaoyang Xiong, Minghang Yu, Xiaoyan Ding, et al.density gradient centrifugation method and prepared as single-cell suspensions. All samples were stained with viability dye (423110, BioLegend) after pre-blocking with an Fc receptor blocking antibody (70–0161, Tonbo Biosciences). Subsequently,

(7) A multidimensional analysis of temporomandibular joint and ankle joint erosion in inflammatory arthritisFront ImmunolJuly 18, 2025Darja Andreev, Pauline Porschitz, Daniela Weidner, Rui Song, Matthias Weider, Georg Schett, Lina Gölz, Aline Bozec101320) for 10 minutes at 4°C. Following this, Zombie Red™ Fixable Viability dye (1:2000; BioLegend; Cat# 423110) was added, and cells were stained with an antibody cocktail for 20 minutes at 4°C. The antibodies used

(8) TIGIT deficiency promotes autoreactive CD4+ T-cell responses through a metabolic‒epigenetic mechanism in autoimmune myositisNat CommunMay 15, 2025Yimei Lai, Shuang Wang, Tingting Ren, Jia Shi, Yichao Qian, Shuyi Wang, Mianjing Zhou, Ryu Watanabe, Mengyuan Li, Xinyuan Ruan, et al.the Annexin V-APC/7-AAD Apoptosis Kit (MultiSciences, Cat# 70-AP101) or Zombie Red (Biolegend, Cat# 423110) by flow cytometry. Details of all the FACS antibodies are shown in Supplementary Table 3. All the FACS

(9) Lactobacillus acidophilus potentiates oncolytic virotherapy through modulating gut microbiota homeostasis in hepatocellular carcinomaNat CommunApril 7, 2025Jiayu Zhang, Jinneng Yang, Jinyan Luo, Weili Wu, Haidan Luo, Wenxia Wei, Haimei Lyu, Yuzhi Wang, Hairong Yi, Yijing Zhang, et al.secreted proteins in ex vivo cells. Dead cells were stained with Zombie Red™ Fixable Viability kit (423110, BioLegend, USA). The staining for cell surface antigens was performed. Cells were fixed using True-Nuclear™ Transcription Factor Buffer Set

(10) Tuft cell IL-17RB restrains IL-25 bioavailability and reveals context-dependent ILC2 hypoproliferationNature ImmunologyMarch 12, 2025Xiaogang Feng, Tilde Andersson, Pascal Flüchter, Julia Gschwend, Ivan Berest, Julian L. Muff, Antonie Lechner, Aurelia Gondrand, Patrick Westermann, Nina Brander, et al.CD326-PerCP-Cy5.5 (118220) and IL-17RB-APC (146308). The Zombie Red Fixable Viability Kit (BioLegend, 423110) was used to distinguish dead cells. Cells were fixed with 4% paraformaldehyde (Merck, 47608-250ml-f)

(11) c-Rel drives pancreatic cancer metastasis through Fibronectin-Integrin signaling-induced isolation stress resistance and EMT activationbioRxiv : the preprint server for biologyFebruary 1, 2025Bakırdöğen D, Görgülü K, Xin J, Alcalá S, Ruiz-Cañas L, Frank K, Wu N, Diakopoulos KN, Dai C, Öztürk H, et al.antibody are used. Cells are finally resuspended in either 300μL PBS with 1:5000 Zombie-Red (BioLegend 423110), or 300μL FC buffer with 1:5000 DAPI (Cell Signaling, 4083S), depending on the panel. Before analyses,

(12) IL-21 shapes the B cell response in a context-dependent mannerCell reportsJanuary 28, 2025Kim Y, Manara F, Grassmann S, Belcheva KT, Reyes K, Kim H, Downs-Canner S, Yewdell WT, Sun JC, Chaudhuri J.Scientific,BP337-500 Zombie NIR Fixable Viability Dye,BioLegend,423106 Zombie Red Fixable Viability Dye,BioLegend,423110 Zombie Yellow Fixable Viability Dye,BioLegend,423104 Critical commercial assays,Critical commercial

(13) Combining a WT1 Vaccine (Galinpepimut-S) With Checkpoint Inhibition (Nivolumab) in Patients With WT1–Expressing Diffuse Pleural Mesothelioma: A Phase 1 StudyJTO Clinical and Research ReportsJanuary 1, 2025Prashasti Agrawal, Michael Offin, Victoria Lai, Michelle S. Ginsberg, Prasad S. Adusumilli, Valerie W. Rusch, Jennifer L. Sauter, Teresa Ho, Phillip Wong, Marjorie G. Zaudererserum (Cat. 35-011-CV, Corning). Subsequently, cells were stained with a fixable viability dye (Cat. 423110, BioLegend) and antibodies to surface CD8 (SK1, Cat.560179, BD Biosciences), fixed, permeabilized, washed,

(14) MAP3K1 mutations confer tumor immune heterogeneity in hormone receptor-positive HER2-negative breast cancerJournal of Clinical InvestigationNovember 12, 2024Yu-Wen Cai, Cui-cui Liu, Yan-Wu Zhang, Yi-Ming Liu, Lie Chen, Xin Xiong, Zhi-Ming Shao, Ke-Da YuCell Staining Buffer (BioLegend, Cat 420201) and were stained with Zombie Red Fixable Viability Kit (BioLegend, Cat 423110; 1:1,000 in DMSO) at 4°C for 10 minutes, and then incubated with a monoclonal antibody against CD16/32

(15) Sustained lymphocyte decreases after treatment for early breast cancernpj Breast CancerOctober 21, 2024Julia Dixon-Douglas, Balaji Virassamy, Kylie Clarke, Michael Hun, Stephen J. Luen, Peter Savas, Courtney T. van Geelen, Steven David, Prudence A. Francis, Roberto Salgado, et al.Surface stain,Fluorophore,Dilution,Supplier/reference number Live/dead,PE Texas Red,1:800,Biolegend #423110 CD4,PE,1:200,Biolegend #317410 CD3,AF700,1:100,Biolegend #344822 CD8,BV510,1:100,Biolegend #344732 CD45RA,BV711,1:100,Biolegend

(16) ARID1A suppresses R-loop-mediated STING-type I interferon pathway activation of anti-tumor immunityCellJune 20, 2024Matthew B. Maxwell, Marianne S. Hom-Tedla, Jawoon Yi, Shitian Li, Samuel A. Rivera, Jingting Yu, Mannix J. Burns, Helen M. McRae, Braden T. Stevenson, Katherine E. Coakley, et al.antibodies (BioLegend, BD Biosciences, eBioscience). All samples were stained with Zombie Red (BioLegend, 423110) and incubated with Fc receptor blocking antibody (Tonbo, 70–0161). Intracellular cytokine staining

(17) Single-cell adhesive profiling in an optofluidic device elucidates CD8(+) T lymphocyte phenotypes in inflamed vasculature-like microenvironmentsCell Rep MethodsApril 22, 2024Camargo CP, Alapan Y, Muhuri AK, Lucas SN, Thomas SN.assays ,, MojoSort Mouse CD8a Selection Kit,Biolegend,Cat#480035 Zombie Red Fixable Viability Kit,Biolegend,Cat#423110 Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link,Abcam,Cat#ab269823 ,, Experimental models:

(18) Enhancing therapeutic efficacy in luminal androgen receptor triple-negative breast cancer: exploring chidamide and enzalutamide as a promising combination strategyCancer cell internationalApril 9, 2024Zhao YX, Wang H, Zhang SW, Zhang WX, Jiang YZ, Shao ZM.4 °C. All the antibodies and reagents used for flow cytometry included ZombieRED (ECD, Biolegend, #423110), CD45 (clone 30-F11, Biolegend, #103116), CD3e (clone 145-2C11, Biolegend, #100328), CD4 (clone RM4-5,

(19) The PD-1-PD-L1 pathway maintains an immunosuppressive environment essential for neonatal heart regenerationNat Cardiovasc ResMarch 1, 2024Vargas Aguilar S, Cui M, Tan W, Sanchez-Ortiz E, Bassel-Duby R, Liu N, Olson EN.True-Stain Monocyte Blocker (BioLegend, 426103) and incubated with Zombie Fixable Dyes (BioLegend, 423110) to label dead cells for 15 min at room temperature in the dark. Cells were stained in FACS buffer with

(20) Stressed target cancer cells drive nongenetic reprogramming of CAR T cells and solid tumor microenvironmentNature CommunicationsSeptember 15, 2023Yufeng Wang, David L. Drum, Ruochuan Sun, Yida Zhang, Feng Chen, Fengfei Sun, Emre Dal, Ling Yu, Jingyu Jia, Shahrzad Arya, et al.2H7, 1:100), HLA-DR-APC (cat# 307609, clone: L243, 1:100), and Zombie Red™ Fixable Viability Kit (cat# 423110) were purchased from Biolegend. CD3-FITC (cat#555339, clone: HIT3a, 1:100), and FITC-Labeled Human B7-H3

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(1)

11/22/2022Really Good Viability Kit Maria GonzalezI use this antibody to detect my live vs dead lymphocytes when I do FACS analysis. In this particular experiment, I used it on ex-vivo C57BL/6 mouse splenocytes. The cells were stained with Zombie Red... Read Review

Zombie Red™ Fixable Viability Kit from BioLegend

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Zombie Red™ Fixable Viability Kit from BioLegend

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