Free Fatty Acid Uptake Assay Kit (Fluorometric) ab176768 from Abcam

Product Specs
ItemFree Fatty Acid Uptake Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab176768
Quantity100 Test
Sample TypeAdherent cells
Assay Time1h 0m
Research AreaMetabolism

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Macrophage-derived IL-6 reprograms lipid metabolism to promote colorectal cancer development through USP14-mediated FASN deubiquitinationJournal of Translational MedicineFebruary 26, 2026Liu Yunxin, Xian Xirui, Zhu Xinyi, Wang Chenjing, Zhang Ting, Fang Kanghui, Yu Haixia, Chang Zude, Huang Wan, Zhu Yubing, et al.with triglyceride (Cat#ab65336, Abcam, Cambridge, UK) and free fatty acid quantification kits (Cat#ab176768, Abcam) and measured with a microplate reader (PerkinElmer, USA) according to the manufacturer’s instructions. Western blot analysis

(2) Altered Carnitine Homeostasis Modulates Hyperosmolarity-Induced Mitochondrial Dysfunction and Lipotoxicity in Human Corneal Epithelial CellsInvestigative Ophthalmology & Visual ScienceFebruary 19, 2026Richard Kontoh-Twumasi, Antonella Aliste, Alexander Scheid, Karina Lax, Joshua Glass, Ashok Sharma, Shruti Sharmaabove. Free fatty acid (FFA) uptake was assessed using a commercially available kinetic assay kit (ab176768; Abcam, Cambridge, UK) according to the manufacturer's instructions. Fluorescence intensity was measured

(3) GCN5 drives MASLD progression through LXRα/SREBP1c signaling pathway–mediated de novo lipogenesisNature CommunicationsFebruary 16, 2026Hai-Tao Xiao, Peng Song, Jing Jin, Yong-Dan Wang, Yin-Yue Xu, He Wu, Xue-Xue Shao, Qi-Feng Gan, Jian He, Yan-Rong Wen, et al.uptake The uptake of free fatty acids (FFA) was quantified utilizing a fluorometric assay kit (Abcam, ab176768). HL-7702 cells were seeded at 1 × 105 cells per well, resuspended in PBS, and maintained at 37 °C in

(4) Supplementation of L-aspartate corrects MASLD and MASH in mice by inhibiting platelet–hepatocyte interaction-mediated mitochondrial fragmentation via the ATP–P2X7–NEK7–DRP1 axisExperimental & Molecular MedicineFebruary 13, 2026Wen-Jie Cao, Rui Su, Hui-Ling Fu, Jun-Jie Wu, Lin-sheng Huang, Fei-fei Liu, Jin Liu, Zhong-Ping Jiang, Cong-Jun Xu, Yong Rao, et al.and then the medium was replaced with fresh culture medium containing the TF2-C12 FA probe (Abcam, ab176768) at 0.5 μΜ. The fluorescence was measured at an excitation wavelength of 485 nm and an emission wavelength

(5) Exercise-induced CLCF1 attenuates age-related muscle and bone decline in miceNat CommunMay 22, 2025Jae Sook Kang, Jung Ha Kim, Min Ju Kim, Byungkuk Min, Seung-Min Lee, Ga-Yeon Go, Ji-Won Kim, Seongwan Kim, Ju Yeon Kwak, Sung-Wook Chun, et al.(100 ng/mL) for 24 h. FFA uptake was then assessed using the Free Fatty Acid Uptake Assay Kit (Abcam, ab176768), according to the manufacturer’s instructions. Briefly, after CLCF1 treatment, cells were incubated

(6) Golgi condensation causes intestinal lipid accumulation through HIF-1α-mediated GM130 ubiquitination by NEDD4Experimental & Molecular MedicineFebruary 3, 2025Hyunsoo Kim, Channy Park, Xiaofan Wei, Arun Chhetri, Laxman Manandhar, Gyuho Jang, Jaetaek Hwang, Batchingis Chinbold, Chagtsalmaa Chuluunbaatar, Hyug Moo Kwon, et al.incubated at 37 °C. Intracellular free fatty acid uptake was measured using a free fatty acid uptake kit (ab176768, Abcam). Transfection The p(HA)HIF1alpha (P402A, P564A) and p(HA)HIF1alpha (401delta603)R27G plasmids

(7) Upregulation of Hepatic Glutathione S-Transferase Alpha 1 Ameliorates Metabolic Dysfunction-Associated Steatosis by Degrading Fatty Acid Binding Protein 1International journal of molecular sciencesMay 7, 2024Jiang J, Li H, Tang M, Lei L, Li HY, Dong B, Li JR, Wang XK, Sun H, Li JY, et al.acid uptake and transportation activities were assessed using the Free Fatty Acid Uptake Assay Kit (#ab176768, Abcam) following the manufacturer’s instructions. Briefly, L02 cells were transfected with pcDNA3.1(+)

(8) Neuronal growth regulator 1 promotes adipocyte lipid trafficking via interaction with CD36Journal of lipid researchJune 1, 2022Yoo A, Joo Y, Cheon Y, Lee SJ, Lee S.DMEM for 1 h. Fatty acid uptake was assessed using a Free Fatty Acid Uptake Assay kit (catalog no.: ab176768; Abcam, Cambridge, UK) according to the manufacturer’s instructions. Fluorescence signals were measured

(9) SLC25A1 promotes tumor growth and survival by reprogramming energy metabolism in colorectal cancerCell death & diseaseNovember 27, 2021Yang Y, He J, Zhang B, Zhang Z, Jia G, Liu S, Wu T, He X, Wang N.acid uptake assay Fatty acid uptake was evaluated with a Free Fatty Acid Uptake Assay Kit (Abcam, ab176768) according to the manufacturer’s protocol. Briefly, CRC cells were plated in a 96-well plate at a density

(10) S100A4 enhances protumor macrophage polarization by control of PPAR-γ-dependent induction of fatty acid oxidationJournal for ImmunoTherapy of CancerJune 18, 2021Shuangqing Liu et al.Darmstadt, Germany). FA uptake assay FA uptake was measured using a free FA uptake assay fluorometric kit (ab176768, Abcam, Cambridge, UK). Cells (1×105/well) were resuspended in Hank’s balanced salt solution and incubated

(11) Targeting the metabolic vulnerability of acute myeloid leukemia blasts with a combination of venetoclax and 8-chloro-adenosineJournal of hematology & oncologyApril 26, 2021Buettner R, Nguyen LXT, Morales C, Chen MH, Wu X, Chen LS, Hoang DH, Hernandez Vargas S, Pullarkat V, Gandhi V, et al.control. Fatty acid uptake was evaluated via the Free Fatty Acid Uptake Assay Kit (Fluorometric) (Cat# ab176768; Abcam). Briefly, after incubation with the drugs, cells were washed with phosphate-buffered saline

(12) TANK-Binding Kinase 1 Regulates the Localization of Acyl-CoA Synthetase ACSL1 to Control Hepatic Fatty Acid OxidationCell metabolismDecember 1, 2020Huh JY, Reilly SM, Abu-Odeh M, Murphy AN, Mahata SK, Zhang J, Cho Y, Seo JB, Hung CW, Green CR, et al.Fisher,Cat#TR13421 CloneAmp Hifi DNA PCR premix,Takara,Cat#639298 Free fatty acid uptake assay kit,Abcam,Cat#ab176768 GST protein purification kit,Pierce,Cat#16107 In-Fusion HD enzyme premix,Takara,Cat#639690 Ketone body assay kit

(13) Metabolic Maturation Media Improve Physiological Function of Human iPSC-Derived CardiomyocytesCell reportsJuly 21, 2020Feyen DAM, McKeithan WL, Bruyneel AAN, Spiering S, Hörmann L, Ulmer B, Zhang H, Briganti F, Schweizer M, Hegyi B, et al.coated 384-well plate. The fatty acid was evaluated with the Free Fatty Acid Uptake Assay Kit (Abcam, ab176768) as recommended by manufacturer’s protocol. Cells were washed 3x with serum-free media and preincubated

(14) Enhanced Lipid Accumulation and Metabolism Are Required for the Differentiation and Activation of Tumor-Associated MacrophagesCancer ResearchApril 1, 2020Pan Su, Qiang Wang, Enguang Bi, Xingzhe Ma, Lintao Liu, Maojie Yang, Jianfei Qian, Qing YiBioscience). Lipid uptake assay Fatty acid uptake was measured using a Free Fatty Acid Uptake Assay kit (ab176768, Abcam, USA), following the manufacturers’ protocol. Briefly, cells were seeded at a density of 50,000

(15) Deficiency of the Circadian Clock Gene Bmal1 Reduces Microglial ImmunometabolismFrontiers in immunologyJanuary 1, 2020Wang XL, Wolff SEC, Korpel N, Milanova I, Sandu C, Rensen PCN, Kooijman S, Cassel JC, Kalsbeek A, Boutillier AL, et al.FBS free culture medium. At the end of treatment, 100 µl fatty acid dye (TF2-C12)-loading solution (ab176768, Abcam) was incubated with microglia for 1 h at 37°C. Fluorescent signals were detected by microplate

(16) miR-142 controls metabolic reprogramming that regulates dendritic cell activationThe Journal of clinical investigationApril 8, 2019Sun Y, Oravecz-Wilson K, Bridges S, McEachin R, Wu J, Kim SH, Taylor A, Zajac C, Fujiwara H, Peltier DC, et al.transfection. FA uptake assay. Uptake of FAs was measured using a free FA uptake assay fluorometric kit (Abcam, ab176768). DCs (1 × 105/ well), either unstimulated or stimulated with indicated TLR ligands, were resuspended

(17) New Insight on Solute Carrier Family 27 Member 6 (SLC27A6) in Tumoral and Non-Tumoral Breast CellsInternational Journal of Medical SciencesJanuary 24, 2019Meng-Chi Yen, Shih-Kai Chou, Jung-Yu Kan, Po-Lin Kuo, Ming-Feng Hou, Ya-Ling HsuThe fatty acid uptake was evaluated via the Free Fatty Acid Uptake Assay Kit (Fluorometric) (cat. no. ab176768; Abcam, UK). After phosphate‑ buffered saline (PBS) washing and 1-hour preincubated in serum‑free media,

(18) Solute Carrier Family 27 Member 4 (SLC27A4) Enhances Cell Growth, Migration, and Invasion in Breast Cancer CellsInternational Journal of Molecular SciencesNovember 1, 2018Meng-Chi Yen, Shih-Kai Chou, Jung-Yu Kan, Po-Lin Kuo, Ming-Feng Hou, Ya-Ling HsuFree Fatty Acid Uptake Assay Kit (Fluorometric) according to the manufacturer’s instructions (cat. no. ab176768; Abcam, Cambridge, UK). Before fatty acid uptake assay, 1 × 104 Hs578T and MDA-MB-231 were seeded on

(19) Regulatory mechanism of fatty acid‑CoA metabolic enzymes under endoplasmic reticulum stress in lung cancerOncology ReportsAugust 21, 2018Kuan‑Ting Liu, I‑Jeng Yeh, Shih‑Kai Chou, Meng‑Chi Yen, Po‑Lin KuoFree Fatty Acid Uptake Assay Kit (Fluorometric) according to the manufacturer's instructions (cat. no. ab176768; Abcam). Before fatty acid uptake assay, 2x10 4 A549 and CL1‑0 cells were seeded on a 96‑well plate

(20) The Foxo1-Inducible Transcriptional Repressor Zfp125 Causes Hepatic Steatosis and HypercholesterolemiaCell ReportsJanuary 9, 2018Gustavo W. Fernandes et al.(free + incorporated in triglyceride) assay was measured similarly, using an FA uptake assay (Abcam, ab176768). Cells were plated in 96-well black plates with 100 μL media/well and incubated overnight in 0.1% FBS

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Free Fatty Acid Uptake Assay Kit (Fluorometric) from Abcam

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Free Fatty Acid Uptake Assay Kit (Fluorometric) from Abcam

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