Fig 1: Expression analysis of selected markers of complement system in wtABCC6HepIm (n = 2), htABCC6HepIm (n = 1), and chtABCC6HepIm (n = 1). mRNA expression of (A) hC1R and (B) hC3 was determined by qRT-PCR. (C) C1R and (D) C3 content in cell culture supernatant was measured with ELISA and normalized to DNA content. Data are shown as mean ± SEM. Mann–Whitney U significance test levels: p < 0.001 (***) and p < 0.0001 (****).