Human HIF-1 alpha ELISA Kit ab171577 from Abcam

Product Specs
ItemHuman HIF-1 alpha ELISA Kit
CompanyAbcam
Price $515.00Supplier Page View Company Product Page
Catalog Numberab171577
ApplicationssELISA, ELISA
Gene NameHIF1A
Detection Range0.23 - 15 ng/ml
SensitivityEqual to 42 pg/mL
NCBI Gene AliasesHIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
NCBI Full Gene Namehypoxia inducible factor 1 subunit alpha
Research AreaEpigenetics
Quantity1 x 96 Tests

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(1) A dynamic model of gene activation in response to hypoxia accounting for both HIF-1 and HIF-2Brief Funct GenomicsDecember 12, 2025Aleksandra Cabaj, Agata Charzyńska, Adrianna Moszyńska, Maciej Jaśkiewicz, Rafał Bartoszewski, Michał Dąbrowskihypoxia for HIF2A. Cell lysis and further ELISA was performed using Human HIF-1-alpha ELISA Kit (Abcam, ab171577), and Human HIF-2-alpha ELISA Kit (Abcam, ab227898) according to the manufacturer’s protocols. Total

(2) Thymoquinone Overcomes Hypoxia-Induced Carboplatin Resistance Through ROS-Independent Apoptosis but Promotes Cancer Stem Cell Enrichment: Implications on Oral Cancer Adaptation and RecurrencePharmaceuticals (Basel)November 18, 2025Ishrat Rahman, Hanan Henidi, Manal M. Alkahtani, Zaha Al Makhlafi, Sahar ElRefai, Manal A. AlSheddi, Rizwan Ali, Sara K. Albassam, Hazar S. Alharbi, Maha G. Omar, et al.stored at − 90 °C until use. 4.3. HIF-1a ELISA ELISA was conducted using a HIF-1a Human ELISA kit (ab171577, Abcam, Cambridge, UK) on OECM-1 cell lysates (1 mg/mL) from untreated cells (basal) in normoxic and

(3) Harnessing adrenergic blockade in stress-promoted TNBC in vitro and solid tumor in vivo: disrupting HIF-1α and GSK-3β/β-catenin driven resistance to doxorubicinFrontiers in PharmacologyJune 19, 2024Yasmeen Attia, Andrew Hakeem, Rawda Samir, Aya Mohammed, Abdullrahman Elsayed, Alaa Khallaf, Eman Essam, Hossameldeen Amin, Sarah Abdullah, Salwan Hikmat, et al.CAR-treated cell lysates following the manufacturers’ instructions of the human HIF-1α ELISA kit (Cat. no.: ab171577; Abcam, United States) and the β-catenin ELISA Kit (Cat. no.: MBS164367; MyBioSource, United States),

(4) Distinct molecular targets of ProEGCG from EGCG and superior inhibition of angiogenesis signaling pathways for treatment of endometriosisJournal of Pharmaceutical AnalysisJanuary 1, 2024Sze Wan Hung, Massimiliano Gaetani, Yiran Li, Zhouyurong Tan, Xu Zheng, Ruizhe Zhang, Yang Ding, Gene Chi Wai Man, Tao Zhang, Yi Song, et al.ELISA kits were purchased. Human VEGFA ELISA kit (ab119566, Abcam) and Human HIF-1 alpha ELISA kit (ab171577, Abcam) were used to test the vascular endothelial growth factor A (VEGFA) and hypoxia-inducible factor

(5) Carvacrol Arrests the Proliferation of Hypopharyngeal Carcinoma Cells by Suppressing Ornithine Decarboxylase and Hyaluronidase ActivitiesFrontiers in NutritionApril 8, 2022Kaneez Fatima, Suaib Luqman, Abha Meenaby a kit purchased from ELab Sciences, USA. The VEGF (Catalog No. ab100663) and HIF-1α (Catalog No. ab171577) were estimated using a kit procured from Abcam, UK. Cell Cycle Analysis, Dichloro-Dihydro-Fluorescein

(6) Inhibition of HMGB1 Might Enhance the Protective Effect of Taxifolin in Cardiomyocytes via PI3K/AKT Signaling PathwayIran J Pharm ResSeptember 27, 2021Erjun Feng, Jian Wang, Xinwei Wang, Zhenguo Wang, Xiaochu Chen, Xu Zhu, Wenli Houab75762) were purchased from Abcam. FGF21 (ab222506), VEGF-α (ab119566), TGF-β (ab100647), HIF-1α (ab171577) ELISA kits were purchased from Abcam (Cambridge, UK). Construct of vectors. Full-length of HMGB1 cDNA

(7) Optimal Hypoxic Preconditioning of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells (hES-MSCs) and Their CharacteristicsInternational journal of stem cellsMay 30, 2021Lee SM, Jun DW, Kang HT, Oh JH, Saeed WK, Ahn SB.under normal and low O2 conditions were collected in a microtube, and 1× Cell Extraction Buffer PTR (ab171577, Abcam, Cambridge, UK) was used for protein extraction. The protein extracts were quantified, and 20

(8) Low oxygen tension differentially regulates the expression of placental solute carriers and ABC transportersFEBS LettersMarch 1, 2021Ludwik Gorczyca, Jianyao Du, Kristin M. Bircsak, Xia Wen, Anna M. Vetrano, Lauren M. Aleksunesanalysis. HIF-1α protein concentration was determined by HIF-1α ELISA kit, as per manufacturer’s protocol (ab171577, Abcam). Data were normalized to protein concentration. Western Blot. Following treatment, BeWo cells

(9) Hypoxia Regulates DPP4 Expression, Proteolytic Inactivation, and Shedding from Ovarian Cancer CellsInternational Journal of Molecular SciencesOctober 30, 2020Laura R. Moffitt, Maree Bilandzic, Amy L. Wilson, Yiqian Chen, Mark D. Gorrell, Martin K. Oehler, Magdalena Plebanski, Andrew N. Stephenscell culture supernatant were performed using commercially available ELISA kits (Human HIF-1-alpha; ab171577 and Human CD26; ab11951, Abcam, Cambridge, UK) as per the manufacturer’s instructions. CD26 ELISA was

(10) Expression and predictive value of HIF‑1α and VEGF in patients with burns following treatmentExperimental and Therapeutic MedicineOctober 5, 2020Zhufeng Liu, Ling Liu, Xuejuan Cheng, Liming Gaofor 10 min to separate the serum and then stored in the refrigerator at -80˚C. ELISA kits (cat. nos. ab171577 and ab233625; Abcam), was employed to determine HIF-1α and VEGF levels. The dilution concentrations

(11) Association of Nuclear Receptor Coactivators with Hypoxia-Inducible Factor-1α in the Serum of Patients with Chronic Kidney DiseaseBioMed Research InternationalAugust 20, 2020Tianbiao Zhou, Wenshan Lin, Shujun Lin, Zhiqing Zhong, Yuanyuan Luo, Zhijun Lin, Weiji Xie, Weitao Shen, Kai Hongenzyme-linked immunosorbent assay method, and all the reagents were purchased from Abcam, Co., USA (HIF-1α: ab171577; VEFG: ab222510) and Dogesce (NCOA1: DG95699Q; NCOA2: DG95703Q; and NCOA3: BioMed Research International DG95709Q).

(12) Chemically Induced Hypoxia Enhances miRNA Functions in Breast CancerCancersJuly 22, 2020Gervin E, Shin B, Opperman R, Cullen M, Feser R, Maiti S, Majumder M.Enzyme-Linked Immunosorbent Assay (ELISA) Analysis of HIF-1α HIF-1α protein quantification was carried out using the ab171577-HIF1a Human SimpleStep ELISA Kit (Abcam, Toronto, ON, Canada). This assay is specific to the HIF-1α

(13) The brains of six African mole-rat species show divergent responses to hypoxiaJournal of Experimental BiologyJanuary 1, 2020Samantha M. Logan, Kama E. Szereszewski, Nigel C. Bennett, Daniel W. Hart, Barry van Jaarsveld, Matthew E. Pamenter, Kenneth B. StoreyELISA HIF-1α protein abundance was determined with a commercially available HIF-1α ELISA kit (Abcam, ab171577). All buffers, reagents and standards were prepared according to the manufacturer’s instructions. First,

(14) Longer Hydroxyurea Administration Prior to Imatinib Mesylate is Risk Factor for Unsuccessful Major Molecular Response in Chronic-Phase Chronic Myeloid Leukemia: Possibility of P-Glycoprotein RoleAsian Pacific Journal of Cancer PreventionDecember 1, 2019Ikhwan Rinaldi, Ary Harryanto Reksodiputro, Sri Widia Jusman, Alida Harahap, Rianto Setiabudy, Septelia Inawati Wanandi, Karmel Tambunan, Catharina Suhartievaluated by enzymed-linked immunosorbent assay (ELISA) using a kit from Abcam with a catalogue number of ab171577. The examination of P-gp expression was carried out using quantitative real-time reverse transcription

(15) Ascorbic acid attenuates cell stress by activating the fibroblast growth factor 21/fibroblast growth factor receptor 2/adiponectin pathway in HepG2 cellsMolecular medicine reportsSeptember 1, 2019Gu X, Luo X, Wang Y, He Z, Li X, Wu K, Zhang Y, Yang Y, Ji J, Luo X.of cells. The concentration of HIFα was measured using a commercially available ELISA kit (cat. no. ab171577, Abcam) according to the manufacturer's instructions. RNA interference. The small interfering RNA (siRNA) sequences

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Brief Funct Genomics

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Human HIF-1 alpha ELISA Kit from Abcam

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Human HIF-1 alpha ELISA Kit from Abcam

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