GFP ELISA Kit ab171581 from Abcam

Product Specs
ItemGFP ELISA Kit
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab171581
Quantity1 96TESTS
ApplicationsELISA
Gene NameGFP
TypeSandwich (quantitative)
Detection Range2.7 - 2000 pg/ml
Sensitivity1.8 pg/ml
Detection TargetGFP

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Multiomics analysis of naturally efficacious lipid nanoparticle coronas reveals high-density lipoprotein is necessary for their functionNature CommunicationsJuly 6, 2023Kai Liu, Ralf Nilsson, Elisa Lázaro-Ibáñez, Hanna Duàn, Tasso Miliotis, Marie Strimfors, Michael Lerche, Ana Rita Salgado Ribeiro, Johan Ulander, Daniel Lindén, et al.treatments as indicated in Supplementary Table 2, eGFP levels were measured with a GFP ELISA Kit (#ab171581, Abcam) according to the manufacturer’s instruction. Briefly, the liver, spleen and kidney tissues were

(2) Sequential verification of exogenous protein production in OVA gene-targeted chicken bioreactorsPoultry scienceJanuary 1, 2023Kim YM, Shim JH, Park JS, Choi HJ, Jung KM, Lee KY, Park KJ, Han JY.quantities of OVA and EGFP were measured using OVA ELISA kit (CSB-E13315C, Cusabio, TX) and GFP ELISA kit (ab171581, Abcam, Cambridge, UK) according to the manufacturer's instructions. Structural and Morphological Analysis

(3) Development of a low-seroprevalence, αvβ6 integrin-selective virotherapy based on human adenovirus type 10Molecular Therapy - OncolyticsJune 16, 2022Emily A. Bates et al.TissueRuptor (Qiagen). Protein was extracted from frozen tissue as recommended for the GFP SimpleSTEP ELISA (ab171581; Abcam, Cambridge, UK), and GFP was measured according to the kit protocol. Total protein was determined

(4) Tuning protein half-life in mouse using sequence-defined biopolymers functionalized with lipidsProceedings of the National Academy of SciencesJanuary 25, 2022Koen Vanderschuren, Pol Arranz-Gibert, Minsoo Khang, Dagan Hadar, Alice Gaudin, Fan Yang, Ewa Folta-Stogniew, W. Mark Saltzman, Miriam Amiram, Farren J. Isaacsanalysis. ELP-GFP concentrations of the samples were determined using a GFP ELISA Kit (Abcam, catalog #ab171581). The samples were diluted in PBS as needed, to ensure that the concentration fell within the quantifiable

(5) Proteosomal degradation impairs transcytosis of AAV vectors from suprachoroidal space to retinaGene TherapyDecember 1, 2021Kun Ding, Jikui Shen, Sean Hackett, Mahmood Khan, Peter A. CampochiaroDevices, San Jose, California). GFP protein levels were measured using a GFP SimpleStep ELISA kit (ab171581, Abcam, Cambridge, MA). Briefly, 50 μl of sample or GFP standard dilutions were added to duplicate wells

(6) Transfer of oral bacteria to the fetus during late gestationScientific ReportsDecember 1, 2021Kevin Yu, Michelle Rodriguez, Zubin Paul, Elizabeth Gordon, Tongjun Gu, Kelly Rice, Eric W. Triplett, Maureen Keller-Wood, Charles E. Woodconcentrations of Green Fluorescent Protein was assayed using a commercially available ELISA kit (Abcam #ab171581, Cambridge, UK). Frozen samples of fetal cerebral cortex (150–250 mg) were placed into conical centrifuge

(7) Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET AssessmentsACS Pharmacology & Translational ScienceJune 11, 2021Paweł Kozielewicz, Rawan Shekhani, Stefanie Moser, Carl-Fredrik Bowin, Janine Wesslowski, Gary Davidson, Gunnar Schulteconcentration and integrity of eGFP-WNT-3A in the CM samples were determined using ELISA (GFP ELISA kit, #ab171581, Abcam) and SDS-PAGE/Western Blot analysis, respectively. Two eGFP-WNT-3A batches (eGFP-WNT-3A batch

(8) A versatile platform for generating engineered extracellular vesicles with defined therapeutic propertiesMolecular TherapyMay 1, 2021Kevin Dooley et al.purified from 200-mL production cultures were diluted 500-fold in 1× cell extraction buffer (CEB, Abcam ab171581). EV stock concentrations ranged from 5.5E+11–5.0E+12 p/mL before initial dilution. EV samples were

(9) Chlorella vulgaris Extract as a Serum Replacement That Enhances Mammalian Cell Growth and Protein ExpressionFrontiers in Bioengineering and BiotechnologySeptember 15, 2020Jian Yao Ng, Mei Ling Chua, Chi Zhang, Shiqi Hong, Yogesh Kumar, Rajeev Gokhale, Pui Lai Rachel EeEnzyme-Linked Immunosorbent Assay (ELISA) Quantification of intracellular GFP was conducted with Abcam GFP SimpleStep ELISA kit (ab171581, Cambridge, United Kingdom) on cell lysates that were harvested using CelLytic M, according to the manufacturer’s

(10) Engineered Capsids for Efficient Gene Delivery to the Retina and CorneaHuman Gene TherapyJuly 1, 2020Amy Frederick, Jennifer Sullivan, Lin Liu, Matthew Adamowicz, Michael Lukason, Jasmine Raymer, Zhengyu Luo, Xiaoying Jin, Kollu Nageswara Rao, Catherine O'Riordanhomogenized at 4C (Fisher Bead Mill). The eGFP protein was quantified using the GFP ELISA Kit (cat. no. ab171581; Abcam). Total protein levels were quantified using the BCA Protein Assay Kit (Pierce). The levels of

(11) Suprachoroidal gene transfer with nonviral nanoparticlesScience advancesJuly 1, 2020Shen J, Kim J, Tzeng SY, Ding K, Hafiz Z, Long D, Wang J, Green JJ, Campochiaro PA.(#5000006, Bio-Rad, Hercules, CA). GFP protein levels were measured using a GFP SimpleStep ELISA kit (ab171581, Abcam, Cambridge, MA). Briefly, 50 μl of sample or GFP standard dilutions was added to duplicate wells

(12) eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length FrizzledThe Journal of biological chemistryJune 26, 2020Wesslowski J, Kozielewicz P, Wang X, Cui H, Schihada H, Kranz D, Karuna M P, Levkin P, Gross JC, Boutros M, et al.and integrity of eGFP-Wnt-3a in the CM samples were determined using ELISA (GFP ELISA® kit, Abcam, ab171581). WB analysis For Western blot (WB) analysis of Wnt-3a and eGFP-Wnt-3a, proteins were first purified

(13) A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson's disease potential biomarkersTranslational neurodegenerationJanuary 1, 2020Anjo SI, Dos Santos PV, Rosado L, Baltazar G, Baldeiras I, Pires D, Gomes A, Januário C, Castelo-Branco M, Grãos M, et al.Human Vacuolar protein sorting-associated protein 35 (VPS35) ELISA Kit (ref. abx384246, Abbexa) and GFP ELISA Kit (ab171581, Abcam), respectively. All the procedures were performed as indicated by the manufacturer. VPS35 determination

(14) AAV8-vectored suprachoroidal gene transfer produces widespread ocular transgene expressionJournal of Clinical InvestigationOctober 7, 2019Kun Ding et al.Microplate Reader (Molecular Devices). GFP protein levels were measured using a GFP SimpleStep ELISA kit (ab171581, Abcam). Briefly, 50 μL sample or GFP standard dilutions was added to duplicate wells of 96-well plates,

(15) Proof of principle: Physiological transfer of small numbers of bacteria from mother to fetus in late-gestation pregnant sheepPloS oneJanuary 1, 2019Yu K, Rodriguez MD, Paul Z, Gordon E, Rice K, Triplett EW, Keller-Wood M, Wood CE.(Olympus Life Science). GFP ELISA GFP ELISA was performed using the GFP SimpleStep ELISA kit (Abcam #ab171581, Cambridge, UK). Approximately 200mg frozen tissue samples were set in 600mL chilled cell extraction

(16) Development of an inducible anti-VEGF rAAV gene therapy strategy for the treatment of wet AMDScientific ReportsDecember 1, 2018Christopher A. Reid, Emily R. Nettesheim, Thomas B. Connor, Daniel M. Lipinskiprotein levels were quantified using commercially available mCherry-specific (ab221829) and GFP-specific (ab171581) ELISA kits (Abcam, Cambridge, MA). In each sample, GFP protein levels were normalized to mCherry protein

(17) Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platformJournal of Biological EngineeringDecember 1, 2016Georg Wandrey, Joel Wurzel, Kyra Hoffmann, Tobias Ladner, Jochen Büchs, Lorenz Meinel, Tessa LühmannBlue G250 and Bradford Protein Assay Kit were from Pierce (Rockford, USA). GFP ELISA Kit Simple step (#ab171581) was from Abcam (Cambridge, United Kingdom). Acetonitrile (HPLC grade) and trifluoroacetic acid (HPLC

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GFP ELISA Kit from Abcam

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GFP ELISA Kit from Abcam

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