GFP ELISA Kit ab171581 from Abcam

Product Specs
ItemGFP ELISA Kit
CompanyAbcam
Price $515.00Supplier Page View Company Product Page
Catalog Numberab171581
Quantity1 x 96 Tests, 10 x 96 Tests, 1 x 384 Tests
Gene NameGFP
Detection Range18.8 - 1200 pg/ml
SensitivityEqual to 2.8 pg/mL
Research AreaResearch Tools
ApplicationssELISA, ELISA

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Breathing lung-on-chip: a versatile tool for assessing respiratory toxicity across multiple therapeutic modalitiesArchives of ToxicologyApril 1, 2026Linnea Johansson, Giulia Raggi, James Cartwright, Johnny Lindqvist, Laurène Froment, Patrik Andersson, Catherine Betts, Jorrit J. Hornberg, Nina Hobi, Anna Ollerstam, et al.cellular GFP levels Quantification of GFP in cell lysates was performed using GFP ELISA kit from Abcam (ab171581) according to manufactures protocol specified for adherent cell lysates. Epithelial and PBMC cells were

(2) Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivityNeuronMarch 12, 2026Colleen N. McLaughlin, Hui Ji, Katherine X. Dong, Chuanyun Xu, Kenneth Kin Lam Wong, Zhuoran Li, David J. Luginbuhl, Charles Xu, Cheng Lyu, Wei Qin, et al.transgenes were performed on one L3 central nervous system according to the manufacturers protocol (Abcam ab171581) with one key difference: whole brain lysates were prepared in PBS + 0.1% Tween according to Jay et

(3) DNA‐Loaded Nanoparticles Reprogram the Tumor Immune Microenvironment to Treat Brain TumorsSmall SciJanuary 5, 2026Joanna Yang, Divyaansh Raj, Hasan Slika, Aanya Shahani, Leonardo Cheng, Manav Jain, Ethan Idnani, Kathryn M. Luly, FNU Ruchika, Caitlin Kraft, et al.lobe with the tumor. GFP concentration was measured using the provided protocol from a GFP ELISA Kit (Ab171581, Abcam; Cambridge, UK). In Vivo Heterotopic IOMM‐Lee survival 16 hu‐PBMC‐NSG MHC I/II Double Knockout

(4) Highly Efficient Air Sterilization via Low‐Temperature Interfacial Evaporation in Inductively Heated Superhydrophilic Ferromagnetic FiltersAdvanced ScienceNovember 1, 2025Arnau Fons, Cristina Vaca, Eloi Franco‐Trepat, Aritz Lafuente, José Luis Tajada, Albert Serrà, Jorge Diaz Pedroza, Sandra Franco, Rytis Boreika, Itziar Erkizia, et al.Human SARS‐CoV‐2 N ELISA Kit (Thermo Fisher Scientific, ref EH490RB) was employed, and, for RSV, the GFP ELISA Kit (ABCAM, ref ab171581). Initial viral input was 2897 ng mL−1 of p24 gag for SARS‐CoV‐2 pseudoviruses, 13.7 ng mL−1 of NC for

(5) JAK-STAT-activated, fratricide-resistant CAR-T cells targeting membrane-bound TNF effectively treat AML and solid tumorsJ Immunother CancerJuly 13, 2025Takahiro Nakashima, Tsunenori Ouchida, Yuichi Ishikawa, Yusuke Ito, Taeko Hayakawa, Toshiaki Yoshikawa, Haosong Zhang, Hitomi Kasuya, Yang Li, Tetsuya Matsukawa, et al.Tokyo, Japan). Each population was lysed, and EGFP concentration was quantified using a GFP ELISA kit (ab171581, Abcam, Cambridge, UK). The number of EGFP molecules per cell was calculated by dividing the total number

(6) A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activityNat CommunJune 30, 2025Liza Malong, Jessica Roskosch, Carolina Hager, Jean-Philippe Fortin, Roland Schmucki, Marinella G. Callow, Christian Weile, Valentina Romeo, Christoph Patsch, Scott Martin, et al.samples, protein extraction was performed with the reagents from the GFP ELISA kit (Abcam, Catalog No. ab171581), following the manufacturer’s protocol. Briefly, snap frozen tissues were lysed in 300 µl of a solution

(7) Residual DNA impurities in AAV vectors—nature and transcriptionMol Ther Methods Clin DevJune 4, 2025Hsin-I Jen, Patrick Wilkinson, Xiaohui Lu, Wei Zhangextraction buffer PTR containing cell extraction enhancer solution from the GFP ELISA Kit (Abcam, Cat: ab171581). Halt protease inhibitor cocktail (Thermo Fisher Scientific Cat:78430) was also added to the solution.

(8) Scalable production and purification of engineered ARRDC1-mediated microvesicles in a HEK293 suspension cell systemSci RepMarch 1, 2025Kristin Luther, Ali Navaei, Leah Gens, Carson Semple, Pearl Moharil, Ilaria Passalacqua, Komal Vyas, Qiyu Wang, Shu-Lin Liu, Lucy Sun, et al.and then lysed in presence of the extraction enhancer provided with the GFP ELISA kit (Abcam cat # ab171581). The ELISA was done according to the manufacturer’s protocol. Cell lysates were assayed undiluted.

(9) Burn injury–induced G-CSF secretion reduces spic+ erythroblastic island macrophages in the bone marrow and impairs medullary erythropoiesisThe Journal of ImmunologyFebruary 1, 2025John G. Noel, Holly Goetzman, Satarupa Sengupta, Mario Medvedovic, Katie G. Seu, Jason C. Gardnerinto sample wells of a CD163 ELISA (R&D Systems, catalog no. DY7435) or GFP ELISA (Abcam, catalog no. AB171581) for quantification. Whole marrow lysate results are reported as ng/2 femurs and cell lysate results

(10) Ascitic Shear Stress Activates GPCRs and Downregulates Mucin 15 to Promote OvarianCancer MalignancyResearch squareNovember 25, 2024Mehta G, Horst E, Cotter L, Bonini M, Novak C, Treacher N, Zhang Y, Jackson Z, Narayanan IV, Wuchu F, et al.MUC15 concentrations were measured by the University of Michigan Immunology Core using GFP (Abcam; ab171581) and MUC15 (Cusabio; CSB-EL015218HU and Abbexa; abx250464) ELISA kits as per the manufacturer’s protocol. Genetic

(11) PlexinA1 (PLXNA1) as a novel scaffold protein for the engineering of extracellular vesiclesJournal of Extracellular VesiclesNovember 7, 2024Hang Zhao, Zhi Li, Da Liu, Jiaxun Zhang, Zhicheng You, Yuzhang Shao, Hongyan Li, Jun Yang, Xiang Liu, Miaozhu Wang, et al.(ELISA) According to the instructions, GFPs of EVs and CLs were quantified using a GFP Elisa kit (ab171581, Abcam, USA). Following this quantification, the GFP concentrations of EVs and CLs were calculated,

(12) Targeting cargo to an unconventional secretory system within megakaryocytes allows the release of transgenic proteins from plateletsJournal of Thrombosis and HaemostasisNovember 1, 2024Nathan L. Asquith, Isabelle C. Becker, Mark T. Scimone, Thais Boccia Da Costa, Virginia Camacho, María N. Barrachina, Shihui Guo, Daniela Freire, Kellie Machlus, Sol Schulman, et al.was retrieved and frozen down. eGFP levels in the supernatant were assessed using a GFP ELISA (Abcam ab171581). Emission was assessed at a plate reader (Biotek Synergy, Agilent). Results PDI is expressed in

(13) An in vitro validation system for chicken bioreactors using immortalized chicken oviductal epithelial cellsPoultry ScienceJune 1, 2024Kyung Min Jung, Eunhui Yoo, Jae Yong Hanimaging system (Bio-Rad, Hercules, CA). Quantification of EGFP was performed using a GFP ELISA kit (ab171581; Abcam, Cambridge, UK) according to the manufacturer's instructions. Reverse Transcription-Polymerase Chain Reaction

(14) PolyGR and polyPR knock-in mice reveal a conserved neuroprotective extracellular matrix signature in C9orf72 ALS/FTD neuronsNature NeuroscienceApril 1, 2024Carmelo Milioto, Mireia Carcolé, Ashling Giblin, Rachel Coneys, Olivia Attrebi, Mhoriam Ahmed, Samuel S. Harris, Byung Il Lee, Mengke Yang, Robert A. Ellingford, et al.(Helmholtz Zentrum 2 µg ml−1) for capture and detection. GFP levels were measured by the GFP ELISA Kit (ab171581, Abcam) according to manufacturer’s instructions. qPCR with reverse transcription Tissues were dissected

(15) High temperature delays and low temperature accelerates evolution of a new protein phenotypeNat CommunMarch 29, 2024Jia Zheng, Ning Guo, Yuxiang Huang, Xiang Guo, Andreas Wagnerprotocol to quantify the amount of soluble fluorescent proteins in each solution by using a GFP ELISA kit (ab171581, Abcam), which can detect GFP from Aequorea Victoria and its enhanced and superfold variants. Single-molecule

(16) Suprachoroidal gene transfer with nonviral nanoparticles in large animal eyesScience advancesMarch 8, 2024Shen J, Lima E Silva R, Zhang M, Luly KM, Hackett SF, Tzeng SY, Lowmaster SM, Shannon SR, Wilson DR, Green JJ, et al.serum albumin (BSA) as the standard. GFP protein levels were measured using a GFP SimpleStep ELISA kit (ab171581, Abcam, Cambridge, MA). Briefly, 50 μl of sample or GFP standard dilutions was added to duplicate wells

(17) pH-dependent structural transitions in cationic ionizable lipid mesophases are critical for lipid nanoparticle functionProc Natl Acad Sci U S ADecember 12, 2023Julian Philipp, Aleksandra Dabkowska, Anita Reiser, Kilian Frank, Rafał Krzysztoń, Christiane Brummer, Bert Nickel, Clement E. Blanchet, Akhil Sudarsan, Mohd Ibrahim, et al.Cell Fractions. The eGFP protein levels were quantified using a commercial kit purchased from Abcam (ab171581). An extraction buffer (recommended by the kit vendor) was added to the cell samples, that were spun

(18) Multiomics analysis of naturally efficacious lipid nanoparticle coronas reveals high-density lipoprotein is necessary for their functionNature CommunicationsJuly 6, 2023Kai Liu, Ralf Nilsson, Elisa Lázaro-Ibáñez, Hanna Duàn, Tasso Miliotis, Marie Strimfors, Michael Lerche, Ana Rita Salgado Ribeiro, Johan Ulander, Daniel Lindén, et al.treatments as indicated in Supplementary Table 2, eGFP levels were measured with a GFP ELISA Kit (#ab171581, Abcam) according to the manufacturer’s instruction. Briefly, the liver, spleen and kidney tissues were

(19) Influence of Host Age on Intracranial AAV9 TauP301L Induced TauopathyJournal of Alzheimer's disease : JADJanuary 1, 2023Tetlow AM, Jackman BM, Alhadidy MM, Perumal V, Morgan DG, Gordon MN.(Fisher Scientific, Cat. No. KHB7031). The detergent soluble fraction was used to quantify GFP (Abcam Cat. No. Ab171581). Samples were first diluted 1000x in RIPA buffer and then further diluted in the buffer provided by

(20) Sequential verification of exogenous protein production in OVA gene-targeted chicken bioreactorsPoultry scienceJanuary 1, 2023Kim YM, Shim JH, Park JS, Choi HJ, Jung KM, Lee KY, Park KJ, Han JY.quantities of OVA and EGFP were measured using OVA ELISA kit (CSB-E13315C, Cusabio, TX) and GFP ELISA kit (ab171581, Abcam, Cambridge, UK) according to the manufacturer's instructions. Structural and Morphological Analysis

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GFP ELISA Kit from Abcam

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GFP ELISA Kit from Abcam

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