E3 Ligase Auto-Ubiquitylation Assay Kit ab139469 from Abcam

Product Specs
ItemE3 Ligase Auto-Ubiquitylation Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab139469
Sample TypePurified protein
Quantity10 Test

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(1) The p53 R181C mutation accumulates through impaired deacetylation by Sirt1 and facilitates tumor developmentCommunications BiologyJanuary 3, 2026Erli Wang, Liangzi Cao, Wendong Guo, Xiang Dong, Wanying Zhang, Xiaowen Zhang, Zhuo Wang, Xia Chen, Aicun Li, Yu Zou, et al.(E2), and Ub E3 Ligase Buffer which all provided by the E3 Ligase Auto-Ubiquitination Assay Kit (Abcam, ab139469). After incubation at 37°C for 1 h, the reaction was then terminated with 2×SDS loading buffer and subjected

(2) The deubiquitination-PARylation positive feedback loop of the USP10-PARP1 axis promotes DNA damage repair and affects therapeutic efficacy of PARP1 inhibitorOncogeneMay 2, 2025Jingwei Liu, Siyi Zhang, Liangzi Cao, Naijin Zhang, Qiqiang Guo, Yu Zou, Ruohan Yang, Shiyuan Dong, Lixia Zheng, Yutong Xiao, et al.(E2) and Ub E3 Ligase Buffer which all provided by the E3 Ligase Auto-Ubiquitination Assay Kit (Abcam, ab139469). After incubation at 37 °C for 1 h, the reaction was then terminated with 2× SDS loading buffer and

(3) Ubiquitination of NS1 Confers Differential Adaptation of Zika Virus in Mammalian Hosts and Mosquito VectorsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2024Huang C, Jiang T, Pan W, Feng T, Zhou X, Wu Q, Ma F, Dai J.Beads 4FF (Smart‐Lifesciences, Cat # SA010025), E3 Ligase Auto‐Ubiquitylation Assay Kit (Abcam, Cat # ab139469), NSC2805 (MedChemExpress, Cat # HY‐134417). Plasmids The open reading frames of specific viral and

(4) Polyubiquitylated rice stripe virus NS3 translocates to the nucleus to promote cytosolic virus replication via miRNA-induced fibrillin 2 upregulationPLoS pathogensMarch 1, 2024Zhang L, Li Y, Kuhn JH, Zhang K, Song Q, Liu F.ubiquitin ligase auto-ubiquitylation The E3 ligase auto-ubiquitylation assay was performed with an Abcam (ab139469) kit using purified recombinant LsRING protein according to the manufacturer’s instructions. The reaction

(5) ABLIM1, a novel ubiquitin E3 ligase, promotes growth and metastasis of colorectal cancer through targeting IĸBα ubiquitination and activating NF-ĸB signalingCell death and differentiationFebruary 1, 2024He Y, Shi Q, Ling Y, Guo H, Fei Y, Wu R, Tang C, Zhang X, Yao L.the proteins was validated by SDS-PAGE. According to instructions from the manufacturer (Abcam, Cat# ab139469), recombinant ABLIM1 1-401aa, 376-778aa, and negative PBS control were incubated with 20 × E1, 20 ×

(6) The deubiquitinase ZRANB1 is an E3 ubiquitin ligase for SLC7A11 and regulates ferroptotic resistanceThe Journal of cell biologyNovember 6, 2023Huang S, Zhang Q, Zhao M, Wang X, Zhang Y, Gan B, Zhang P.according to the manufacturer’s instructions using an in vitro E3 ligase autoubiquitylation assay kit (ab139469; Abcam). Briefly, assay components were added to 0.5 ml Eppendorf tube(s), including distilled H2O,

(7) O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N(6)-methyladenosine-dependent mannerSignal transduction and targeted therapyFebruary 10, 2023Yang Y, Yan Y, Yin J, Tang N, Wang K, Huang L, Hu J, Feng Z, Gao Q, Huang A.buffer (20 mM Tris-HCl pH = 7.4, 5 mM MgCl2, 1 mM DTT) according to manufacturer’s instructions (Abcam, ab139469). The reaction was performed at 37 °C for 2 h and stopped by 2×SDS-PAGE buffer. After that, the complexes

(8) Dismissal of RNA Polymerase II Underlies a Large Ligand-Induced Enhancer Decommissioning ProgramMolecular CellAugust 16, 2018Yuliang Tan et al.Mg-ATP Solution and Ub E3 Ligase Buffer supplied by E3 Ligase Auto-Ubiquitination Assay Kit (Abcam, ab139469). ChIP and ChIP-seq Briefly, cells were cross-linked with 1% formaldehyde at room temperature for

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E3 Ligase Auto-Ubiquitylation Assay Kit from Abcam

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E3 Ligase Auto-Ubiquitylation Assay Kit from Abcam

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