Kinase-Glo Max Luminescent Kinase Assay V6071 from Promega

Product Specs
ItemKinase-Glo Max Luminescent Kinase Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV6071
Detection TargetKinase activity
Quantity10ml
MethodLuminescent Assay

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Machine Learning‐Guided Engineering of Protein Phase Separation Properties in Immune RegulationAdvanced ScienceFebruary 11, 2026Chenqiu Zhang, Jia Wang, Zhe Wang, Liyan Zhu, Sihui Cai, Luzhi Zhan, Haorui Liang, Yaoxing Wu, Jianqiang Li, Jun CuiIn Vitro cGAMP processing ability of cGAS in vitro was measured with a Kinase‐Glo Luminescent Kinase Assay (V6071, Promega) according to the manufacturer's instructions. 4.15 Quantification of the Abundance of cGAS‐Bound

(2) Ruthenium-enhanced curcumin derivatives target tumor growth and cancer-related inflammation in head and neck cancer modelsFront OncolDecember 17, 2025Kateřina Veselá, Ameneh Tatar, Zdeněk Kejík, Nikita Abramenko, Robert Kaplánek, Petr Babula, Kateřina Kučnirová, Jan Hajduch, Pavel Martásek, Milan Jakubekor curcumin (5, 10, and 20 μM) according to the manufacturer’s protocol. Kinase-Glo® MAX (Promega, #V6071, Madison, Wisconsin, USA) was used as the detection reagent, with an incubation time of 15 min. Blank

(3) Discovery of Novel Piperidinyl-Based Benzoxazole Derivatives as Anticancer Agents Targeting VEGFR-2 and c-Met KinasesPharmaceuticals (Basel)December 9, 2025Wagdy M. Eldehna, Zainab M. Elsayed, Mohamed R. Elnagar, Ahmed H. El-Said, Taghreed A. Majrashi, Ahmed T. Negmeldin, Abdulrahman M. Saleh, Ranza Elrayess, Khaled A. Elnahriry, Zhi-Long Chen, et al.of 50 mL. The reaction mixture was incubated at 30 °C for 45 min, and then Kinase-Glo MAX (Promega #V6071) reagent (50 mL) was added to each well of the reaction plate. A BioTekTM Synergy 2 microplate reader

(4) Design, synthesis and molecular modeling of new coumarin–thiazole derivatives as dual EGFR/HDAC1 inhibitors: in vitro and in vivo anticancer assaysRSC AdvancesSeptember 5, 2025Eman Y. Ahmed, Mai M. Elghonemy, Rasha Z. Batran, Manar E. A. Elasasy, Sherien M. El-Daly, Marwa A. Mahmoud, Hanem M. Awad, Nehad A. Abdel LatifEGFR kinase assay kit (BPS Bioscience, CA # 40321). The kit uses Kinase-Glo™ MAX as the detection reagent (Promega #V6071). Results of the tested compound were assessed by measuring the luminescence using a ROBONIK P2000 ELIZA

(5) Selective Dual Inhibition of TNKS1 and CDK8 by TCS9725 Attenuates STAT1/β-Catenin/TGFβ1 Signaling in Renal CancerCurrent Issues in Molecular BiologyJune 17, 2025Majed Saad Al Fayi, Mishari Alshyarbacompound XAV-939 (#27100) were purchased from BPS Bioscience, San Diego, CA, USA. Kinase-GloTM Max (#V6071) was from Promega Corporation, Madison, WI, USA. The QCM™ Tumor Cell Trans-Endothelial Migration Assay

(6) Aqueous extracts of Portulaca oleracea L. alleviate atopic dermatitis by restoring skin barrier functionFront PharmacolJune 5, 2025Haobin Wei, Zhang Chen, Wenjie Lai, Wenxian Wang, Xiqing Bian, Limin Zhang, Xinzhi Liaurantiamide acetate on JAK1 tyrosine kinase activity. The assay employed ADP-Glo™ as a detection reagent (V6071, Promega) and was conducted via a luminescence plate reader (SpectraMax iD3, Molecular Devices) following

(7) Hepato-selective dihydroquinolizinones active against hepatitis A virus in vitro and in vivoAntiviral ResearchMay 1, 2025Ichiro Misumi, Zhizhou Yue, Zhengyuan Jiang, Anilkumar Karampoori, Jason K. Whitmire, John M. Cullen, Timothy Block, Stanley M. Lemon, Yanming Du, You Litemperature for 1 h (TENT4B) or 3 h (TENT4A). Residual ATP was measured by Kinase-Glo max reagent (Promega #V6071) with luminescence quantified using a Biotek Synergy II multi-mode plate reader (BioTek Instruments). Human

(8) Structural insight into the cGAS active site explains differences between therapeutically relevant speciesCommun ChemMarch 22, 2025Alexander M. Skeldon, Li Wang, Nicolas Sgarioto, Ramsay E. Beveridge, Silas Chan, Stephane Dorich, Valerie Dumais, Nadine Fradet, Samuel Gaudreault, Philippe LeGros, et al.assessed using a luminescence-based readout of ATP consumption, determined by Kinase-Glo Max (Promega # V6071). Enzyme concentrations of 40 nM h-cGAS, 0.5 nM m-cGAS, 10 nM m-cGAS (I309T), 1.5 nM rat cGAS, 15 nM

(9) Dibenzo [a, c] phenazin-11-yl(phenyl) methanone (SBLJ23), a novel selective inhibitor targeting JAK2^V617F mutation in myeloproliferative neoplasmsOncology ResearchFebruary 28, 2025MOHAMMAD ABOHASSAN, MESFER MOHAMMAD AL SHAHRANI, SARAH KHALED ALOUDA, PRASANNA RAJAGOPALANat 30°C for 45 min. Following this, 50 µL of Kinase-Glo MAX (Promega, Madison, Wisconsin, MA, USA #V6071) was added to each well and incubated at room temperature for 15 min. Luminescence was recorded using

(10) Rational Design and Synthesis of a Novel Series of Thiosemicarbazone-Containing Quinazoline Derivatives as Potential VEGFR2 InhibitorsPharmaceuticsFebruary 15, 2025Alexandru Șandor, Ovidiu Crișan, Gabriel Marc, Ionel Fizeșan, Ioana Ionuț, Cristina Moldovan, Anca Stana, Ilioara Oniga, Adrian Pîrnău, Laurian Vlase, et al.luminescence detection, after the addition of the Kinase-Glo™ MAX reagent (Promega, Madison, WI, USA, catalog #v6071). After further incubation for an additional 15 min, the luminescence was recorded using a microplate

(11) Chemical genetics reveals cross-regulation of plant developmental signaling by the immune peptide-receptor pathwaySci AdvFebruary 7, 2025Arvid Herrmann, Krishna Mohan Sepuru, Pengfei Bai, Hitoshi Endo, Ayami Nakagawa, Shuhei Kusano, Asraa Ziadi, Hiroe Kato, Ayato Sato, Jun Liu, et al.ATP, which was then converted to a luminescent signal by the luciferase reaction (Promega, catalog no. V6072). First, the MPK6 protein alone or together with MKK5DD was incubated in 30 μl of reaction buffer: 25

(12) SBL-JP-0004: A promising dual inhibitor of JAK2 and PI3KCD against gastric cancerOncology ResearchJanuary 1, 2025HASSAN M. OTIFIincubating at 30°C for 45 min. Subsequently, 50 μL of Kinase-Glo MAX (Promega Corporation, Madison, WI, USA #V6071) was added to each well and incubated at room temperature for 15 min. Luminescence was recorded using

(13) The mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognitionNature CommunicationsDecember 30, 2024Bingyi Chen, Fang Yi, Zhiteng Luo, Feihu Lu, Hongwei Liu, Siting Luo, Qiong Gu, Huihao Zhoumin) were transferred to a 384 well plate and mixed with 5 μL of Kinase-Glo® Max Reagent (Cat. No. V6071, Promega) to stop the reaction. After 20 min, the luminescence (L) which reflects the concentration

(14) Chemical genetics reveals cross-activation of plant developmental signaling by the immune peptide-receptor pathwaybioRxivAugust 2, 2024Arvid Herrmann, Krishna Mohan Sepuru, Hitoshi Endo, Ayami Nakagawa, Shuhei Kusano, Pengfei Bai, Asraa Ziadi, Hiroe Kato, Ayato Sato, Jun Liu, et al.ATP, which was then converted to a luminescent signal by the luciferase reaction (Promega, catalog No. V6072). Firstly, MPK6 protein alone or together with MKK5DD were incubated in 30 μL of reaction buffer: 25

(15) Drug Repositioning via Graph Neural Networks: Identifying Novel JAK2 Inhibitors from FDA-Approved Drugs through Molecular Docking and Biological ValidationMoleculesMarch 19, 2024Muhammad Yasir, Jinyoung Park, Eun-Taek Han, Won Sun Park, Jin-Hee Han, Wanjoo Chundegrees Celsius for 45 min. Then, 50 µL of the Kinase-Glo MAX reagent (Promega, Madison, WI, USA, #V6071) was added and covered the plate with aluminum foil, and incubated at room temperature for 15 min. Finally,

(16) OsCPK12 phosphorylates OsCATA and OsCATC to regulate H2O2 homeostasis and improve oxidative stress tolerance in ricePlant CommunicationsMarch 11, 2024Beifang Wang, Pao Xue, Yingxin Zhang, Xiaodeng Zhan, Weixun Wu, Ping Yu, Daibo Chen, Junlin Fu, Yongbo Hong, Xihong Shen, et al.and truncated OsCPK12 were performed using a Kinase-Glo Luminescent Kinase Assay kit (catalog no. #V6071; Promega Biotechnology). Proteins used for kinase activity assays were washed three times (10 min each)

(17) Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylationNature chemical biologyJanuary 1, 2024Chen L, Zhou Q, Zhang P, Tan W, Li Y, Xu Z, Ma J, Kupfer GM, Pei Y, Song Q, et al.reaction based on the PRPS1 activity. Reactions were ended with 10 µl of Kinase-Glo reagent (Promega, V6072) being added to each well to terminate the reaction. Luminescence representing the residual ATP amount

(18) New pyrazolyl-thiazolidinone/thiazole derivatives as celecoxib/dasatinib analogues with selective COX-2, HER-2 and EGFR inhibitory effects: design, synthesis, anti-inflammatory/anti-proliferative activities, apoptosis, molecular modelling and ADME studiesJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2023Wael A. A. Fadaly, Taha H. Zidan, Nesma M. Kahk, Fatma E. A. Mohamed, Marwa M. Abdelhakeem, Rehab G. Khalil, Mohamed T. M. Nemrluminescence. HER-2 inhibitory activity HER-2 inhibitory activity was determined using Kinase-Glo MAX (Promega, #V6071) (Catalog # 40230). IC50 of compounds 16a, 18c, 18d, 18f were determined in MCF7 cells according to

(19) Novel benzo chromene derivatives: design, synthesis, molecular docking, cell cycle arrest, and apoptosis induction in human acute myeloid leukemia HL-60 cellsJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2023Rania H. Abd El-Hameed, Mosaad S. Mohamed, Samir M. Awad, Bardes B. Hassan, Marwa Abd El-Fattah Khodair, Yara E. Mansoursynthesised compounds. The assay was performed using Kinase-Glo MAX luminescence kinase Assay kit (Promega #V6071) (catalogue no. 79599) (BPS Bioscience, San Diego, CA) and followed the manufacturer’s instructions.

(20) EGFR and PI3K/m-TOR inhibitors: design, microwave assisted synthesis and anticancer activity of thiazole–coumarin hybrids†RSC AdvancesOctober 4, 2023Rasha Z. Batran, Eman Y. Ahmed, Hanem M. Awad, Korany A. Ali, Nehad A. Abdel LatifEGFR kinase assay kit (BPS Bioscience, Cat. #40321). The kit uses Kinase-Glo MAX as the detection reagent (Promega #V6071). Results of the tested compound were assessed by measuring the luminescence using a microplate reader. 4.2.3.

Be the First to Write a Review!

Kinase-Glo Max Luminescent Kinase Assay

Kinase-Glo Max Luminescent Kinase Assay from Promega

Description

Promega

Citations (37)

Reviews

Kinase-Glo Max Luminescent Kinase Assay from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map