HiScribeT7 Quick High Yield RNA Synthesis Kit from New England Biolabs

Product Specs
ItemHiScribeT7 Quick High Yield RNA Synthesis Kit
CompanyNew England Biolabs
Price $298.00 Inquire
Catalog NumberE2050S
Quantity50 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potencyNat CommunApril 24, 2026Diana Ly, Hyewon Jang, Adhiraj Goel, Arnav Singh, Aditya Raguramin vitro transcription using the HiScribe® T7 Quick High Yield RNA Synthesis Kit (New England Biolabs; E2050S). Transcribed RNA yield was quantified using the QubitTM RNA High-Sensitivity Assay Kit (Thermo Fisher Scientific

(2) SFPQ Promotes Homologous Recombination via mRNA Stabilization of RAD51 and Its ParalogsJournal of Molecular BiologyApril 15, 2026Sofia Gotthold, Keile R. Hansen, Andrew N. Brown, Soham P. Chowdhury, Christine M. Joyce, Viraj Vinish, Sofie I. Rodriguez, Sambhav Jain, Hannah I. Ghasemi, Amanda C. Yoon, et al.in vitro using T7 RNA polymerase (HiScribe T7 Quick High Yield RNA Synthesis Kit; New England Biolabs, Cat#E2050S), followed by DNase I treatment to remove residual DNA. The 5′ triphosphate groups were removed by incubation

(3) Whole-organism spatial transcriptomics at single-cell resolution in C. elegansbioRxivApril 11, 2026Jose David Aguirre, Xuan Wan, Carsten H. Tischbirek, Nicholas Markarian, Core Francisco Park, Long Cai, Paul W. Sternbergmanufacturer’s instructions. These purified PCR products served as templates for in vitro transcription (NEB, E2050S) and were supplemented with RNasin RNase Inhibitor (Promega, N261A) and Pyrophosphatase (NEB, M0361S). The

(4) Synergistic Inhibition of Porcine Reproductive and Respiratory Syndrome Virus by a Bifunctional 5'-PPP miRNA Combining RIG-I Activation with Sequence-Specific Viral TargetingVirusesMarch 20, 2026Zihang Song, Jiabao Hou, Feng Guo, Longping Chen, Chudong Wang, Xinjie Guo, Ping Li, Wenlong Shen, Jiajun Yang, Hongxu Zhong, et al.using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA, NEB, E2050S) according to the manufacturer’s instructions. DNA templates were generated by annealing complementary

(5) PEGASUS: Unlocking Polarity in Cell-Permeable Cyclic Peptides Using AI Models Built on Massively Parallel Biological AssaysJournal of Medicinal ChemistryMarch 12, 2026Cole Baker, Francis A. Acquah, Lakshmi G. Chivukula, Liping Wu, Laurence Philippe-Venec, Mostafa Abedi, Yujun Tao, Daniel Ramírez, Matthew D. McCoy, Brandon Moore, et al.library DNA was transcribed into mRNA using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB, E2050S) in 30 μL containing 40 units of Recombinant RNase Inhibitor (Takara, 2313A) for 20 min at 37 °C, followed

(6) An advanced rapid-visual CRISPR assay for detecting porcine reproductive and respiratory syndrome virusScientific ReportsMarch 11, 2026Jiajia Guo, Suyu Shi, Shengsong Xie, Lu Jing, Cui Wang, Dagang Taoagarose gel electrophoresis. In vitro transcription was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (E2050S, New England Biolabs) under standard cycling conditions. The synthesized RNA was quantified with the

(7) La protein binding to telomerase RNA supports an evolutionary relationship between plant and ciliate telomerase pathwaysNucleic Acids ResMarch 10, 2026Leon Jenner, Dzmitry Pruchkouski, Barbora Štefanovie, Olga Nováková, Monika Kubíčková, Petr Fajkus, Marie Brázdová, Jan Paleček, Eva Sýkoroválabelling RNA was synthesized using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, #E2050S) to transcribe a PCR product amplified from pCRII TOPO plasmid DNA sequences using primers detailed

(8) Attenuation of epicardial activation and myofibroblast abundance via the Fbln2-Nupr1b axis stimulates cardiac regeneration in zebrafishNat Cardiovasc ResMarch 3, 2026Gülsüm Kayman Kürekçi, Gursimran Kaur Bajwa, Shaoqiu Zhang, Séverine Leclerc, Emilie de Chantal, Darrell Belke, Gregor Andelfinger, Justin F. Deniset, Rubén Marín-Juezin vitro transcription using a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, catalog number E2050S). One-cell-stage fbln2FLD−/− embryos were injected with 100 pg of fbln2 mRNA and collected at 3 days

(9) An image-based transcriptomics atlas reveals the regional and microbiota-dependent molecular, cellular, and spatial structure of the murine gutCell Host MicrobeFebruary 19, 2026Rosalind J. Xu, Hao Zhang, Paolo Cadinu, Phillip B. Nicol, Uli S. Herrmann, Tyrone Lee, Ludwig Geistlinger, Rafael A. Irizarry, Jeffrey R. MoffittD4004). The product was amplified and converted to RNA with a high yield in vitro transcription (NEB E2050S) and purified with home-made solid-phase reversible immobilization beads39 (based on Cytiva 65152105050250).

(10) Advancing knock-in approaches for robust genome editing in zebrafishBiology OpenFebruary 15, 2026Anjelica Rodriguez-Parks, Ella Grace Beezley, Steffani Manna, Isabella Silaban, Sarah I. Almutawa, Siyang Cao, Hossam Ahmed, Megan Guyer, Sean Baker, Mark P. Richards, et al.(Genesee Scientific) to generate sgRNA templates. sgRNAs were synthesized using the HiScribe T7 kit (NEB, E2050S) and purified by the RNA purification Kit (Zymogen, R1016) according to the manufacturer's instructions.

(11) Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potencybioRxivFebruary 2, 2026Diana Ly, Hyewon Jang, Adhiraj Goel, Arnav Singh, Aditya Raguramin vitro transcription using the HiScribe® T7 Quick High Yield RNA Synthesis Kit (New England Biolabs; E2050S). Transcribed RNA yield was quantified using the Qubit™ RNA High-Sensitivity Assay Kit (Thermo Fisher Scientific

(12) A rapid, visual, ultrasensitive and highly specific method for detecting adeno-associated virus 2020 based on the LAMP-CRISPR/Cas12a systemPoultry ScienceFebruary 1, 2026Ping Gong, Dagang Tao, Qianhong Chen, Yu Yang, Shengsong Xie, Xing Chen, Suyu Shi, Yanan Wang, Lixia Wang, Yunguo Qian, et al.were transcribed into sgRNAs using the NEB HiScribe™ T7 Quick High Yield RNA Synthesis Kit (Cat No. E2050S, NEW ENGLAND Biolabs). The resulting sgRNAs were then purified and stored for downstream applications.

(13) The FANCD2-FANCI heterodimer coordinates chromatin openness and cell cycle progression throughout DNA double-strand break repairCell ReportsJanuary 27, 2026Christine M. Joyce, Julien Bacal, Soham P. Chowdhury, Andrew N. Brown, Amy K. Wang, Carmen Cruz, Kameron Bains, Zachary N. Rodriguez, Nathan J. McCormick, Yaara Tzadikario, et al.transcribed into gRNA by a T7 RNA polymerase using the HiScribe T7 Quick High Yield RNA Synthesis Kit (Cat#E2050S; New England Biolabs), followed by removal of the DNA template by treatment with DNase I. Next, 5′ triphosphate

(14) White spot syndrome virus IE1 protein hijacks the host pentose phosphate pathway to fuel viral replicationPLOS PathogensJanuary 27, 2026Jia Zhang, Kaiyu Lu, Jinghua Zhu, Jude Juventus Aweya, Yueling Zhang, Defu Yaoin vitro using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, USA; Cat. No. E2050S), following the manufacturer’s instructions. The primers used for dsRNA synthesis are listed in S1 Table.

(15) Hijacking of host Src-PI3K-Akt signaling by WSSV IE1 protein suppresses apoptotic and autophagic defenses to facilitate viral proliferationJournal of VirologyJanuary 20, 2026Kaiyu Lu, Jia Zhang, Jinghua Zhu, Yongzhen Zhao, Xiuli Chen, Yueling Zhang, Defu Yaoin vitro using the HiScribe Quick High Yield RNA Synthesis Kit (New England Biolabs, USA; Cat. No. E2050S) according to the manufacturer’s protocol, with dsEGFP serving as a negative control. For IE1 knockdown,

(16) Imaging the Intestinal Transcriptome With Multiplexed Error‐Robust Fluorescence In Situ Hybridization (MERFISH)Current ProtocolsDecember 27, 2025Paolo Cadinu, Evan Yang, Rosalind J. Xu, Brianna R. Watson, Josh Luce, Jeffrey R. Moffitt(VWR, N2615) Murine RNase inhibitor (NEB, M0314L) HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, E2050S) Maxima H Minus Reverse Transcriptase (200 U/μL; Thermofisher, EP0753) RNase/DNase-free nuclease-free

(17) Endodermal cells use contact inhibition of locomotion to achieve uniform cell dispersal during zebrafish gastrulationiScienceDecember 19, 2025Jesselynn LaBelle, Tom Wyatt, Jaxson Ramirez, Stephanie Wooassays,Critical commercial assays ,, HiScribe T7 quick High Yield RNA synthesis kit,New England Biolabs,E2050S mMessage mMachine T3 transcription kit,ThermoFisher Scientific,AM1344 RNA Clean and Concentrator-25

(18) Furin-Mediated Cleavage of Zona Pellucida Proteins Is Essential for Oocyte DevelopmentMedComm (2020)December 12, 2025Tiechao Ruan, Xiang Wang, Xueguang Zhang, Yan Wang, Chuan Jiang, Sixian Wu, Yunchuan Tian, Xinyao Tang, Jun Ma, Shikun Zhao, et al.mice, and Zp3 Arg353Ser/+ mice. The gRNAs were synthesized with the HiScribe T7 High Yield RNA system (E2050s, NEB) and subsequently purified using MEGAclear Kit (AM1908, Invitrogen) in vitro. Single‐strand oligodeoxynucleotides

(19) Advancing Knock-In Approaches for Robust Genome Editing in ZebrafishbioRxivDecember 12, 2025Anjelica Rodriguez-Parks, Ella Grace Beezley, Steffani Manna, Isabella Silaban, Sarah I Almutawa, Siyang Cao, Hossam Ahmed, Megan Guyer, Sean Baker, Mark P Richards, et al.(Genesee Scientific) to generate sgRNA templates. sgRNAs were synthesized using the HiScribe T7 kit (NEB, E2050S) and purified by the RNA purification Kit (Zymogen, R1016) according to the manufacturer's instructions.

(20) Nanoscale dynamics of enhancer-promoter interactions during exit from pluripotencyNucleic Acids ResDecember 8, 2025Gabriela Stumberger, David Hörl, Dimitra Tsouraki, Clemens Steinek, A Marieke Oudelaar, Heinrich Leonhardt, Hartmann Harzto RNA via a high yield in vitro transcription (IVT) according to the manufacturer’s instructions (#E2050S, New England Biolabs). Each 30 μl reaction consisted of ∼1 μg template DNA, 6.66 μM of each NTP, 20

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