Description
Product Characteristics: The T4 is a solid phase competitive ELISA. The samples and the diluted T4 enzyme conjugate are added to the wells coated with anti-T4 polyclonal antibody. T4 in the serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate is washed off by wash buffer during a wash step. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is generated relating color intensity to the concentration of the T4.
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