NEBNextMultiplex Small RNA Library Prep Set for Illumina(Set 1) E7300S from New England Biolabs

Product Specs
ItemNEBNextMultiplex Small RNA Library Prep Set for Illumina(Set 1)
CompanyNew England Biolabs
Price $1,515.00 Inquire
Catalog NumberE7300S
Quantity24 reactions
ApplicationsDNA Analysis, Multiplex, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Molecular Insights into the Regulatory Mechanisms Mediated by Hypoxia-Conditioned Skeletal Muscle Exosomal miRNAsBiomarker InsightsFebruary 28, 2026Haijiao Wang, Baochang Shi, Wenting Song, Lu Lu, Akhilesh K. Bajpai, Qiu Lipreparation was conducted using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB #E7300S, USA) following manufacturer protocols with modifications for exosomal RNA input. Briefly, 3 μg of exosomal

(2) A Multi-Tissue Yak (Bos grunniens) ceRNA Atlas with Ribo-Seq–Informed lncRNA Curation and Candidate PrioritizationAnimals (Basel)February 8, 2026Zhenlin Zhu, Biao Li, Mingfeng JiangSmall RNA Library Prep Set for Illumina® (Set 1) (New England Biolabs, Ipswich, MA, USA, Catalog #: E7300S). Briefly, 3′ and 5′ adapters were ligated to the 3′ and 5′ ends of the small RNAs, respectively. First-strand

(3) WDR4-mediate tRNA m7G modification to promote mitophagy and browning of white adipose tissue for ameliorating obesity in male miceAdipocyteDecember 31, 2025Beisi Lin, Chaofan Wang, Yanling Yang, Zhigu Liu, Panwei Mu, Wen Xu, Yonghui Lireverse-transcribed into cDNA using NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) (#E7300S, NEB). The cDNA was amplified using PCR with forward and reverse primers complementary to the 5’ and

(4) Argonaute-siRNA loading via the RNA-binding protein RDE-4 in C. elegansCurrent BiologyDecember 1, 2025Thiago L. Knittel, Brooke E. Montgomery, Reese A. Sprister, Colin N. Magelky, Margaret J. Smith, Maritza Soto-Ojeda, Melissa Guthrie, Carolyn M. Phillips, Taiowa A. MontgomeryScientific",Cat# 4427975 NEBNext Small RNA Library Prep Kit for Illumina,New England Biolabs,Cat# E7300S TaqMan Fast Advanced Master Mix,"Applied Biosystems™, distributed by Thermo Fisher Scientific",Cat#

(5) Divergent proteome tolerance against gain and loss of chromosome armsMolecular CellNovember 20, 2025Yi Di, Wenxue Li, Joan Josep Castellano, Wenjie Jin, Joanna N. Modi, Barbora Salovska, Delyar Khosroabadi, Wei Hu, Alison M. Taylor, Yansheng Liucatalog # RL00P-12)69. The RPFs underwent end repair using the Small RNA Library Prep Kit (NEB; catalog # E7300S) and size selection using the RNA Clean & Concentrator-5 Kit (Zymo; catalog # R1015) to enrich RNA fragments

(6) Cappable-Seq reveals the transcriptional landscape of stress responses in the bacterial endosymbiont WolbachiaMicrob GenomOctober 27, 2025Youseuf Suliman, Zhiru Li, Amit Sinha, Philip D. Dyer, Catherine S. Hartley, Laurence Ettwiller, Alistair C. Darby, Clotilde K. Carlow, Benjamin L. Makepeaceunderwent library preparation using the NEBNext multiplex small RNA library prep kit (NEB product number E7300S) following the manufacturer’s instructions. After library completion, samples underwent PCR clean-up

(7) Transcriptomic Response to Neuromuscular Electrical Stimulation in Muscle, Brain, and Plasma EVs in WT and Klotho-Deficient MiceInt J Mol SciAugust 14, 2025Catherine Anne Cavanaugh, Amanda E. Moore, Nicholas Francis Fitz, Iliya Lefterov, Radosveta Koldamovanon-coding libraries were produced by New England BioLabs’ NEBNext Multiplex Small RNA Library Prep Set (E7300S and E7580S, Ipswich, MA, USA). Library quality was assessed on samples by the High Sensitivity DNA Assay

(8) Small RNA in sperm-Paternal contributions to human embryo developmentNat CommunJuly 17, 2025Signe Isacson, Kajsa Karlsson, Stefan Zalavary, Anna Asratian, Unn Kugelberg, Susanne Liffner, Anita Östused. Small RNA libraries were produced with NEBNext Multiplex SmallRNA Library Prep Kit for Illumina (E7300S, E7330S, E7580S; New England Biolabs), with the customisation of a dilution of primers 1:8. The 3´adaptor

(9) Interdependence of Pasha and Drosha for localization and function of the Microprocessor in C. elegansNat CommunJuly 1, 2025Thiago L. Knittel, Brooke E. Montgomery, Kailee J. Reed, Madeleine C. Chong, Ida J. Isolehto, Erin R. Cafferty, Margaret J. Smith, Reese A. Sprister, Colin N. Magelky, Hataichanok Scherman, et al.ligation step changed to 16 °C for 18 h to improve capture of methylated small RNAs (New England Biolabs, E7300S). ~134–148 nt small RNA PCR amplicons, corresponding to ~16–30 nt small RNAs, were size selected on

(10) Ribosomes binding to TAS transcripts buffer ta-siRNA biogenesis in Arabidopsis thalianaFront Plant SciMay 19, 2025Han Wang, Qiming Wen, Minglei Zhao, Hualong He, Jie Cui, Chenjiang Youlibrary construction was performed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, E7300S), adhering to the manufacturer’s protocol. 4.3 Analysis of sRNA-seq data and calculation of phasing

(11) RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum-chloroplast membrane contact sitesNat CommunFebruary 25, 2025Juan Huang, Juan Du, Yan Liu, Lu Lu, Yanzhuo Xu, Jianfei Shi, Qing Liu, Qi Li, Yang Liu, Yaqiu Chen, et al.used for sRNA libraries construction using the NEBNext® Small RNA library Prep Set for Illumina® (NEB, E7300S). Two biological replicates of sRNA libraries were constructed and sequenced using an Illumina Hiseq2500

(12) A dominant role of transcriptional regulation during the evolution of C4 photosynthesis in Flaveria speciesNat CommunFebruary 14, 2025Ming-Ju Amy Lyu, Huilong Du, Hongyan Yao, Zhiguo Zhang, Genyun Chen, Yuhui Huang, Xiaoxiang Ni, Faming Chen, Yong-Yao Zhao, Qiming Tang, et al.Ribo-seq libraries were prepared using the NEBNext Multiple Small RNA Library Prep Set for Illumina® (NEB, E7300S and E7300L). Sequencing was performed on the Illumina NovaSeq X Plus by Gene Denovo Biotechnology Co.,

(13) Promoter-proximal RNA polymerase II termination regulates transcription during human cell type transitionNature Structural & Molecular BiologyFebruary 11, 2025Kseniia Lysakovskaia, Arjun Devadas, Björn Schwalb, Michael Lidschreiber, Patrick Cramerovernight. RNA libraries were prepared with NEBNext Multiplex small RNA library prep set for Illumina (NEB, E7300S) according to the manufacturer’s instructions. Libraries were size-selected with 4% E-Gel high-resolution

(14) Universal First-Trimester Screening Biomarkers for Diagnosis of Preeclampsia and Placenta Accreta SpectrumBiomoleculesFebruary 4, 2025Timofeeva AV, Fedorov IS, Tarasova AM, Sukhova YV, Kolod'ko VG, Ivanets TY, Sukhikh GT.RNA Library Prep Set for Illumina® (Sets 1 and 2, New England Biolabs®, Frankfurt, Germany, cat. nos. E7300S and E7580S). Libraries were amplified for 19 cycles, purified using the QIAQuick PCR Purification Kit

(15) Decreased SynMuv B gene activity in response to viral infection leads to activation of the antiviral RNAi pathway in C. elegansPLoS BiolJanuary 29, 2025Ashwin Seetharaman, Himani Galagali, Elizabeth Linarte, Mona H. X. Liu, Jennifer D. Cohen, Kashish Chetal, Ruslan Sadreyev, Alex J. Tate, Taiowa A. Montgomery, Gary Ruvkunstep changed to 16°C for 18 h to improve capture of methylated small RNAs (New England Biolabs, cat# E7300S). PCR products corresponding in size to adapter-ligated small RNAs were size selected on a 10% polyacrylamide

(16) Decoding Codon Bias: The Role of tRNA Modifications in Tissue-Specific TranslationInt J Mol SciJanuary 15, 2025Daisuke Ando, Sherif Rashad, Thomas J. Begley, Hidenori Endo, Masashi Aoki, Peter C. Dedon, Kuniyasu NiizumaRNA Library Prep kit for Illumina according to the manufacturer’s protocol (NEB, Ipswich, MA, USA, #E7300S). Pair-end sequencing reads of size 150 bp were produced for Ribo-seq on the Illumina Hiseq X-ten system.

(17) miRNA-target complementarity in cnidarians resembles its counterpart in plantsEMBO ReportsJanuary 2, 2025Yael Admoni, Arie Fridrich, Paris K Weavers, Reuven Aharoni, Talya Razin, Miguel Salinas-Saavedra, Michal Rabani, Uri Frank, Yehu Moranµl,Bio-Rad,3450091 NEBNext Multiplex Small RNA Library Prep Set for Illumina kit,New England Biolabs,NEB-E7300S T4 RNA Ligase 2 truncated KQ,New England Biolabs,M0373S Certified™ Low Range Ultra Agarose,Bio-Rad,1613107 orange

(18) PhpCNF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA productionNat CommunJanuary 2, 2025Manjit Kumar Srivastav, H. Diego Folco, Patroula Nathanailidou, Anupa T Anil, Drisya Vijayakumari, Shweta Jain, Jothy Dhakshnamoorthy, Maura O'Neill, Thorkell Andresson, David Wheeler, et al.PCR amplification were performed using the NEBNext Small RNA Library Prep Set for Illumina (NEB, Cat# E7300S). The final library was sequenced on the MiSeq platform. TSA treatment and dilution assay Cells were

(19) Exosomal miR-320b regulates cardiomyocyte FOXM1 expression and may serve as an early-stage compensatory mechanism in obstructive sleep apneaPloS oneJanuary 1, 2025Wang WY, Chen ZJ, Lai YJ, Guo LL, Xiong A, Huang ZY, Yao XY, Zeng YM.total RNA extracted from exosomes using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (E7300S, New England Biolabs). Briefly, total exosomal RNA underwent 3’ and 5’ adaptor ligation and was amplified

(20) A low-abundance class of Dicer-dependent siRNAs produced from a variety of features in C. elegansGenome ResearchDecember 23, 2024Thiago L. Knittel, Brooke E. Montgomery, Alex J. Tate, Ennis W. Deihl, Anastasia S. Nawrocki, Frederic J. Hoerndli, Taiowa A. MontgomeryRNA Library Prep Set for Illumina following the manufacturer's recommendations (New England Biolabs E7300S). See Supplemental Material for additional details. Small RNA data analysis sRNA-seq data were analyzed

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NEBNextMultiplex Small RNA Library Prep Set for Illumina(Set 1)

NEBNextMultiplex Small RNA Library Prep Set for Illumina(Set 1) from New England Biolabs

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NEBNextMultiplex Small RNA Library Prep Set for Illumina(Set 1) from New England Biolabs

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