NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Blocking the m6Am methyltransferase PCIF1 releases STAT1-mediated Th1 immunity to potentiate cancer immunotherapyNat CommunJuly 22, 2026Jiansong Huang, Jing Zhou, Xiao Liu, Xiaoxu Liu, Ye Xiao, Kai Li, Chenbo Ding, Xuemin Cai, Wenli Mao, Changfen Xu, et al.RNA was subjected to controlled fragmentation using magnesium-based RNA fragmentation buffer (NEB #E6150S) under thermal denaturation conditions (94 °C for 4 min), generating fragments averaging 150 nucleotides

(2) RNASET2 degrades mRNAs that protect against lipotoxicityMolecular MetabolismJune 25, 2026Shuiling Zhao, Stevens Bontemps, Ryan M. Nottingham, Jun Yao, Mariana Acuña, David E. Cohen, Alan M. Lambowitz, Jean E. SchafferLong RNAs were fragmented to ∼200 nucleotides (NEB Next RNA Fragmentation Module, New England Biolabs E6150S). Small and fragmented large RNAs were pooled prior to treatment with T4 Polynucleotide Kinase (Lucigen

(3) Systematic benchmarking of dorado basecalling models for RNA modification detection with highly multiplexed nanopore sequencingNucleic Acids ResJune 17, 2026Gregor Diensthuber, Ivan Milenkovic, Laia Llovera, Ana Milovanovic, Francesco Pelizzari, Eva Maria Novoacoverage across long transcripts by using 150 ng of total RNA, 2µl of RNA Fragmentation Buffer (NEB, E6150S) in a total volume of 20 µl. The reaction was incubated for 1 min at 94°C to obtain a fragment size

(4) IL-1β-mediated interaction between Müller cells and microglia through CXCL1/5-CXCR2 aggravates visual dysfunction in experimental glaucomaJournal of NeuroinflammationMay 21, 2026Xiaoyu Xin, Luying Han, Yuntao Qu, Lujia Zhang, Xiaoli Zhang, Jiarui Li, Bo Lei, Yong-Chen Wang, Zhongfeng WangThermo Fisher Scientific) and fragmented into small pieces using NEBNext® Magnesium RNA Fragmentation Module (E6150S, NEB, USA). Next, the cleaved RNA fragments were transcribed to double strand cDNA and labeled with

(5) Epitranscriptomic regulation of mRNA stability in pivotal transcription factors modulates the second cell fate decisionNature CommunicationsApril 28, 2026Weide Xiao, Li Tang, Mingli Ma, Kuisheng Liu, Jiaxu Lu, Wenqing Ding, Zhile Bai, Xuelian Liu, Xiaochen Kou, Yanhong Zhao, et al.GLORI library protocol70. Briefly, mRNA was fragmented at 94 °C for 4 min using fragment buffer (NEB, E6150S). Fragmented RNA was then mixed in a 40 μl reaction containing 6 μl glyoxal solution (8.8 M, Sigma-Aldrich,

(6) Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity declineJ Anim Sci BiotechnolApril 22, 2026Mingyue Gao, Junnan Zhang, Boxuan Zhang, Xinwei Jiang, Bowen Niu, Conghao Zhong, Fangren Lan, Wenxin Zhang, Ning Yang, Congjiao Sunmagnesium-based fragmentation buffer (NEBNext® Magnesium RNA Fragmentation Module, New England Biolabs, Cat. No. E6150S). First-strand cDNA synthesis was performed using SuperScript™ II Reverse Transcriptase (Invitrogen,

(7) Developmental and geographic transcriptomic variation in Anisakis simplex (s. s.) reveals lncRNA-mediated regulation of mRNA expressionScientific ReportsApril 20, 2026Robert Stryiński, Mateusz Maździarz, Mónica Carrera, Elżbieta Łopieńska-BiernatrRNA-depleted RNA fraction was fragmented using the NEBNext® Magnesium RNA Fragmentation Module (cat. no. E6150S, New England Biolabs, Ipswich, MA, USA) under high-temperature conditions (94 °C for 5–7 min). Fragmented

(8) IGF2BP3-mediated stabilization of RHOA mRNA promotes osteosarcoma proliferation via Hippo signalingTranslational Cancer ResearchMarch 31, 2026Jingbin Wu, Jiahao Xia, Yuan Tao, Yiping Wengto controlled fragmentation through treatment with RNA Fragmentation Reagent (NEB, Beverly, MA, USA; E6150S). Prior to immunoprecipitation, Protein G magnetic beads were prepared by incubating them with anti-N6-methyladenosine (m6A

(9) RNA sequencing and bioinformatics analysis of blood from patients with cortical cataractsBMC OphthalmologyMarch 26, 2026Qian Li, Lili Zhu94 °C for 5–7 minutes in the presence of divalent cations (Magnesium RNA Fragmentation Module, Cat. #E6150S, NEB, USA). The fragmented RNA was reverse transcribed into cDNA using SuperScript™ II Reverse Transcriptase

(10) The bladder cancer m6A landscape is defined by global methylation dilution and focal 3′-UTR hypermethylationEMBO ReportsMarch 23, 2026Jonas Koch, Jinyun Xu, Felix Bormann, Vitor Coutinho Carneiro, Manuel Neuberger, Katja Nitschke, Malin Nientiedt, Philipp Erben, Maurice Stephan Michel, Manuel Rodriguez-Paredes, et al.Kit,Thermo Fisher Scientific,Cat 61006 NEBNext Magnesium RNA Fragmentation Module,New England Biolabs,Cat E6150S RNA Clean & Concentrator-5 kit (DNase I included),Zymo Research,Cat R1013 Glyoxal solution,Sigma-Aldrich,Cat

(11) LENG8 mediates RNA nuclear retention and degradation in eukaryotesMol CellMarch 19, 2026Lusong Tian, Liang Liu, Yoseop Yoon, Lindsey V. Soles, Marielle Valdez, Joshua Jeong, Clinton Yu, Lan Huang, Yongsheng ShiMix,Takara,Cat#638949 Blasticidin,Invivogen,Cat#ant-bl-05 Puromycin,Invivogen,Cat#ant-pr-1 dTAGv-1,Tocris,Cat#6914 Polybrene,Sigma,Cat#TR-1003 Trizol,Invitrogen,Cat#15596026 NEBNext Magnesium RNA fragmentation module,NEB,Cat#E6150S Phusion High-Fidelity PCR Master Mix with HF Buffer,NEB,Cat#M0531L Lonza SF Cell Line 4D-Nucleofector,ThermoFisher,Cat#NC0281111 X

(12) Programmable RNA N 6 ,2´‐O‐Dimethyladenosine EditingAdvanced ScienceMarch 13, 2026Yang Li, Xiangmin Tan, Yaran Liu, Yongquan He, Bo Yuan, Ping Wang, Guangzhi Ma, Mengzhe Guo, Jian Zhou, Qiang Suntotal RNA was fragmented to 200 nt at 94°C for 4 min using the Magnesium RNA Fragmentation Module (NEB, E6150S). The RNA fragments were purified and concentrated by RNA Clean & Concentrator‐25 spin column (Zymo Research

(13) ABA-Induced Transcriptomic Dynamics in Arabidopsis thaliana Anthers: Insights into Pollen Development and FertilityPlants (Basel)March 13, 2026Lu Liu, Huiting Huang, Dexi Shi, Shuo Wang, Ziyi Lin, Fengming Huang, Li Huang, Sue Linusing NEBNext® Magnesium RNA Fragmentation Module (New England Biolabs, Ipswich, MA, USA, Cat. No. E6150S) followed by ribosomal RNA depletion with the Ribo-Zero Gold rRNA Removal Kit (Illumina, San Diego,

(14) Molecular mechanism of gallium nitrate in inhibiting bacterial biofilm formation through pykF modulationPLoS OneMarch 6, 2026Xiaofeng Zhang, Junjie Dong, Bing Wang, Lingqiang Chen, Zhiqiang Gong, Jin Yang, Guizhao Shu, Qi NingmRNA was then fragmented at 94°C for 5–7 min using the NEBNext Magnesium RNA Fragmentation Module (E6150S; New England Biolabs, USA). The fragmented RNA was reverse transcribed into cDNA using SuperScript II Reverse Transcriptase

(15) Stress fiber traction force reshapes chromatin accessibility and YAP binding to direct diverse transcriptional programs in mesenchymal stem cellsMechanobiol MedMarch 4, 2026Jiacheng Lei, Yicen Long, Xiaojing Liu, Zhiqin Chu, Qiang Weimagnesium ion-based RNA fragmentation module (NEBNext Magnesium RNA Fragmentation Module, NEB, cat.E6150S, USA) at 94 °C for 5-7 min. First-strand cDNA was synthesized using SuperScript™ II Reverse Transcriptase

(16) Comprehensive Profiling of N 6 ‐methyladnosine (m 6 A) Readouts Reveals Novel m 6 A Readers That Regulate Human Embryonic Stem Cell DifferentiationAdvanced ScienceMarch 1, 2026Zhou Huang, Rucong Liu, Zibaguli Wubulikasimu, Wanqing Zhao, Jiaqi Huang, Jiaxuan Wang, Tianyuan Zhang, Rui Fan, Wei Kong, Qinghua Cui, et al.purification kit (Thermo fisher, catalog#AM1908). RNA was incubated with fragmentation buffer (NEB, catalog#E6150S) for 4 min at 94°C and glyoxal solution and DMSO were added, and the mixture was incubated for 30 min

(17) RBM15B recognizes H3K79me2 to guide selective m6A-modification of mRNA and enhance oncoprotein translation in MLL-r leukemiaThe EMBO JournalFebruary 2, 2026Tian-Qi Chen, Yu-Meng Sun, Shun-Xin Zhu, Xiao-Tong Chen, Ke-Jia Pu, Heng-Jing Huang, Qi Pan, Jun-Yi Lian, Wei Huang, Ke Fang, et al.Dynabeads Oligo(dT)25 (Promega, Z5300). mRNA was fragmented by incubation at 94 °C for 4 min with fragment buffer (NEB, E6150S). Two hundred nanograms of fragmented RNA were ordered through RNA protection, deamination, and the

(18) Targeting DAP5 Disrupts Alternate Mode of Translational Initiation in Tregs and Potentiates Antitumor ImmunityAdvanced ScienceFebruary 1, 2026Xiaojiang Lai, Guihong Pan, Xixi Feng, Jun Zhang, Yunxia Li, Shanmeizi Zhao, Zhenming Lin, Yun Feng, Wei Bao, Hongyan Yang, et al.RNAs were fragmented into ≈200 bp pieces with the NEBNext Magnesium RNA Fragmentation Module (NEB, Cat#E6150S) and purified with the Monarch RNA Cleanup Kit (NEB, #T2040L). The purified RNA fragments were dissolved

(19) Investigation of myofiber composition changes and molecular mechanisms in rabbit meat quality development during growthBMC GenomicsJanuary 31, 2026Guohua Song, TongYan Zhu, Zhen Zhen, LiYe Chang, Ahamba Ifeanyi Solomon, Jie Jie, ShuHui Wang, Song Bing, XiangGui Dong, ZhanJun Renshort fragments at high temperature using the NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. E6150S, USA) and subsequently reverse transcribed to generate six libraries for mRNA, lncRNA, and circRNA.

(20) Evaluation of Artemisia annua-Scutellaria baicalensis Herb Pair Carbon Dots Interfering NLRP3 Inflammatory Pathway For Effective Against H1N1 Viral Pneumonia in Mice ModelInt J NanomedicineJanuary 23, 2026Qianyun Zhang, Xinyi Huang, Huimei Kong, Qiongyao Liang, Xiaoqing Lv, Yuan Ma, Jingbo Wang, Jing Chenhigh-temperature fragmentation using the NEBNext® Magnesium RNA Fragmentation Module (NEB, Cat. No. E6150S, USA) at 94 °C for 5–7 min. The fragmented RNA was used for cDNA synthesis using SuperScriptTM II Reverse

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