NEBNextMultiplex Small RNA Library Prep Set for Illumina(Set 1) E7300L from New England Biolabs

Product Specs
ItemNEBNextMultiplex Small RNA Library Prep Set for Illumina(Set 1)
CompanyNew England Biolabs
Price $5,149.00 Inquire
Catalog NumberE7300L
Quantity96 reactions
ApplicationsDNA Analysis, Multiplex, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Predicting competition and substrate preferences for targeted microbiome alterationCellApril 17, 2026Oriane Moyne, Grant J. Norton, Mahmoud Al-Bassam, Chloe Lieng, Deepan Thiruppathy, Manish Kumar, Eli Haddad, Yuhan Weng, Manuela Raffatellu, Livia S. Zaramela, et al.KAPA RNA HyperPrep kit,Roche,cat #08100952001 NEBNext Small RNA library preparation set for Illumina,New England Biolabs,cat #E7300L Nextera XT Indexes,Illumina,"cat #15052163, 15052164, 15052165, 15052166" Nextera XT library preparation

(2) Wolbachia-induced cytoplasmic incompatibility triggers intergenerational dysregulation of the small RNA regulatory network in offspringFront MicrobiolMarch 5, 2026Weihao Dou, Tianchu Lilibraries were prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, E7300L). Total RNA served as input, with adapters ligated to both ends of the small RNAs. This was followed

(3) Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axisNature CommunicationsFebruary 24, 2026Haoning Peng, Zhile Wang, Mengyao Wang, Zheyu Ding, Kaixiu Li, Yuqing Wang, Xuejiao Yu, Siyang Song, Yulan Deng, Yi Liu, et al.libraries were constructed using the Multiplex Small RNA Library Prep Set for Illumina (Set1) (NEB, cat. E7300L). The concentration of the final cDNA libraries was measured using a Qubit® 2.0 Fluorometer and adjusted

(4) Multi‐Tissue Omics Analysis Uncovers Translational Regulation Underlying Complex Traits in PigsAdvanced ScienceFebruary 3, 2026Chao Wang, Yuanyuan Zhang, Choulin Chen, Xiaoxian Xu, Shenghua Qin, Junyan Qi, Yongzhou Bao, Huiming Li, Ruipu Chen, Weigang Zheng, et al.were added to both ends of the RFs with NEBNext Multiple Small RNA Library Prep Set for Illumina (NEB, E7300L), followed by reverse transcription and PCR amplification to enrich for PCR products in the 140–160

(5) Metabolic Adaptation and Pulmonary ceRNA Network Plasticity in Orientallactaga sibirica During Water Deprivation StressInt J Mol SciFebruary 1, 2026Yongling Jin, Rong Zhang, Xin Li, Linlin Li, Dong Zhang, Yu Ling, Shuai Yuan, Xueying Zhang, Heping Fu, Xiaodong Wuconstituted the final library. The NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, Cat. No. E7300L) (Ipswich, MA, USA) was utilized. First, 3′ and 5′ adapters were ligated to both ends of small RNAs,

(6) Comprehensive characterization of non-retroviral endogenous viral elements in the rice pest Chilo suppressalisBMC GenomicsDecember 12, 2025Jia-Bao Lu, Yu-Hua Qi, Ying Tian, Qing-Kai Feng, Peng-Peng Ren, Hai-Jian Huang, Yi-Yuan Li, Jian-Ping Chen, Jun-Min Li, Chuan-Xi ZhangcDNA libraries were prepared with the NEB Next® Multiplex Small RNA Library Prep Set for Illumina®(NEB E7300L, New England Biolabs) and subjected to sequencing on an NovaSeq 6000 platform (Novogene, Tianjin, China).

(7) Structural-guided identification of two modulators of β−1,3-glucan synthase FKS1Nature CommunicationsDecember 7, 2025Jialu Li, Jian Li, Angqi Zhu, Xinli Dai, Juxiu Liu, Huayi Liu, Zhili Xia, Yuefan Dong, Wenyu Qian, Lunzhi Dai, et al.prepared by using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs, E7300L/E7850L) and sequenced by Illumina NextSeq 500 system. The quality control of sequencing data was performed

(8) Downregulation of Chromosome 19 miRNA Cluster and the Tumor‐Suppressive Role of miR ‐517a‐3p in ChoriocarcinomaCancer ScienceDecember 1, 2025Yuki Nishiko, Kosuke Yoshida, Yuko Yasui, Akira Yokoi, Masami Kitagawa, Eri Inami, Masato Yoshihara, Kazumasa Mogi, Yukari Nagao, Satoshi Tamauchi, et al.libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (cat. no. E7300L; New England Biolabs) according to the manufacturer's protocol. Subsequently, small RNA libraries were

(9) Early-life macronutrient exposure alters pubertal timing through hypothalamic microRNA networksScientific ReportsNovember 19, 2025Harsh Shah, Sripriya Bulusu, Hetvi Shah, Nehareeka Dan, Ankita Salunke, A. V. Ramachandran, Parth Pandyaof high-quality RNA per sample and the NEBNext® Multiplex Small RNA Library Prep Kit for Illumina (E7300L, New England Biolabs, USA). The protocol involved sequential ligation of 3′ and 5′ adapters, reverse

(10) Mechanisms and Pathophysiological Significance of Insulin Resistance in Offspring With Intrauterine Growth Restriction Mediated by Hepatic GR/miR‐1224 ProgrammingAdvanced ScienceNovember 1, 2025Yongguo Dai, Xiaoling Guo, Pengxia Yu, Dingmei Zhang, Hao Kou, Hui Wangthe miRNA library was generated using NEB Next Multiplex Small RNA Library Prep Set for Illumina (NEB E7300L). Subsequently, library quality was assessed on the Agilent 5400 system (Agilent, USA) and quantified

(11) Plasma Small Extracellular Vesicle microRNAs as Non-Invasive Biomarkers for Lung Cancer DetectionInternational Journal of NanomedicineSeptember 8, 2025Qiaoli Lv, Yangzhong Guo, Xiaoya Xu, Dongyu Liu, Xiaoling Xiong, Qingfeng Wei, Yan Feng, Dadong Zhang, Zhisheng He, Weimin MaoClara, California, USA). Subsequently, a NEBNext Multiplex Small RNA Library Prep Set for Illumina Kit (E7300L, NEB, USA) was used for library construction, following the specific instructions provided in the product

(12) Machine learning-based proteomics profiling of ALS identifies downregulation of RPS29 that maintains protein homeostasis and STMN2 levelCommun BiolAugust 7, 2025Wei Xu, Zhipeng Guo, Yian Guan, Shihui Lv, Xue Gao, Wenchen Luo, Tianlin Cheng, Zhicheng Shao, Bangbao Tao, Tao Wang, et al.followed by library construction using the Multiplex Small RNA Library Prep Set for Illumina (NEB; E7300L). The libraries were subjected to Illumina sequencing with single-end 50 bp (SE50) sequencing. Raw FASTQ

(13) Therapeutic restoration of miR-96 prevents hearing loss in mice through modulation of noise-induced and genetic pathwaysiScienceMay 16, 2025Ruilong Xia, Chenxi Jin, Siying Fei, Tingting Dong, Ting Wen, Fengting Zhu, Yunxin Shi, Qian Zhou, Yong Tao, Changgeng PengUBCCreERT2/+ and UBCCreERT2/+; miR-96 fL/fL mice was used for library preparation according to NEB E7300L instruction and instruction of VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina, repectively.

(14) Small extracellular vesicle miRNAs as biomarkers for predicting antitumor efficacy in lung adenocarcinoma treated with chemotherapy and checkpoint blockadeFrontiers in ImmunologyMarch 31, 2025Si Sun, Fuchuang Zhang, Jiyang Zhang, Hui Yu, Zhihuang Hu, Xiaoya Xu, Xinmin Zhao, Sheng Chen, Yao Zhang, Baoning Nian, et al.to construct the miRNA library using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (E7300L, NEB, USA) according to the manufacturer’s protocol. In brief, the library was obtained by 3′ adaptor

(15) The translational landscape of reactive astrocytes reveals the impact of eIF2B-mediated dysregulation in VWM diseaseNAR Molecular MedicineMarch 29, 2025Shir Mandelboum, Liat Lev-Ari, Andrea Atzmon, Melisa Herrero, Naama Brezner, Daniel Benhalevy, Orna Elroy-Steinwere generated with NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, #E7300L). Next, libraries were size selected by gel electrophoresis on 8% TBE-polyacrylamide gel, with ∼155

(16) Has-miR-30c-1-3p inhibits macrophage autophagy and promotes Mycobacterium tuberculosis survival by targeting ATG4B and ATG9BSci RepMarch 25, 2025Xianglin Peng, Feifei Pu, Fangzheng Zhou, Xiyong Dai, Feng Xu, Junwen Wang, Jing Feng, Ping Xiasequencing libraries utilizing the NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB E7300L). Following reverse transcription and PCR amplification, a cDNA library was obtained. Libraries with

(17) A dominant role of transcriptional regulation during the evolution of C4 photosynthesis in Flaveria speciesNat CommunFebruary 14, 2025Ming-Ju Amy Lyu, Huilong Du, Hongyan Yao, Zhiguo Zhang, Genyun Chen, Yuhui Huang, Xiaoxiang Ni, Faming Chen, Yong-Yao Zhao, Qiming Tang, et al.were prepared using the NEBNext Multiple Small RNA Library Prep Set for Illumina® (NEB, E7300S and E7300L). Sequencing was performed on the Illumina NovaSeq X Plus by Gene Denovo Biotechnology Co., Ltd. (Guangzhou,

(18) ProQ-associated small RNAs control motility in Vibrio choleraeNucleic Acids ResearchFebruary 8, 2025Rabea Ghandour, Daniel Devlitsarov, Phillip Popp, Sahar Melamed, Michaela Huber, Malte Siemers, Thomas Krüger, Olaf Kniemeyer, Andreas Klingl, Axel A Brakhage, et al.DNA (cDNA) libraries were prepared using the NEBNext Small RNA Library Prep Set for Illumina (NEB; E7300L). RIP-seq analysis The cDNA libraries were pooled and sequenced on a NextSeq1000/2000 system in paired-end

(19) New insights into the salt-responsive regulation in eelgrass at transcriptional and post-transcriptional levelsFront Plant SciFebruary 6, 2025Huan Zhao, Xu Dong, Dazuo Yang, Qingchao Ge, Peng Lu, Chang Liu(Zhang M. et al., 2023; Hu et al., 2021). We used NEB Next® Multiplex Small RNA Library Prep Set (NEB E7300L) for small RNA library construction. The 5 ‘end of small RNA has a complete phosphate group and the

(20) Profiling of small RNAs derived from tomato brown rugose fruit virus in infected Solanum lycopersicum plants by deep sequencingFrontiers in MicrobiologyJanuary 30, 2025Mangle Chen, Donghai Wang, Jiali Yang, Yuhao Cao, Xuemei Song, Yuwen Lu, Hongying Zheng, Jiejun Peng, Guanwei Wu, Jian Wu, et al.confirming the quality of the samples, the NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB E7300L) was used to construct six libraries. In brief, the unique structures at the 3′ and 5′ ends of small

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NEBNextMultiplex Small RNA Library Prep Set for Illumina(Set 1)

NEBNextMultiplex Small RNA Library Prep Set for Illumina(Set 1) from New England Biolabs

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NEBNextMultiplex Small RNA Library Prep Set for Illumina(Set 1) from New England Biolabs

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