5 DNA Adenylation Kit E2610S from New England Biolabs

Product Specs
Item5 DNA Adenylation Kit
CompanyNew England Biolabs
Price $129.00 Inquire
Catalog NumberE2610S
Quantity10 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) 5-Azacytidine incorporation into mRNAs disrupts translation and induces ribosome collisionsbioRxivMarch 31, 2026Alexis B Roberson, James Marks, Ruby Pitts, Bavavarshini Tamilselvam, Brian Grieb, William P Tansey, Sezen Meydanlinkers containing a 5 nt unique molecular identifiers (UMIs) were adenylated with Mth RNA ligase (NEB, E2610). Monosome footprints, disome footprints, and size markers were first dephosphorylated with T4 PNK (NEB,

(2) Multiplexed TrAEL-seq captures DNA replication dynamics in mammalian cellsNucleic Acids ResMarch 14, 2026Neesha Kara, Laura Biggins, Alex Whale, Kieron May, Vera Grinkevich, Paola Garran-Garcia, Jhanavi Srinivasan, Peter J Rugg-Gunn, Claudia Ribeiro de Almeida, Samantha J Walker, et al.phosphate and /iBiodT/ indicates Biotin-dT. Adaptors were adenylated using the 5′ DNA adenylation kit (NEB, E2610S) as follows: 500 pMol DNA oligonucleotide, 5 µl 10 × 5′ DNA Adenylation Reaction Buffer, 5 µl 1 mM ATP,

(3) Collision-induced ribosome degradation driven by ribosome competition and translational perturbationsNat CommunDecember 12, 2025Sihan Li, Okuto Shounai, Misaki Kato, Ken Ikeuchi, Toshifumi InadaT4 Polynucleotide Kinase (# M0201, NEB). Linker oligonucleotides were pre-adenylated using the 5′ DNA Adenylation Kit (# E2610, NEB) and ligated to the dephosphorylated RNA fragments using T4 RNA Ligase 2, Truncated (# M0242, NEB).

(4) Principles of cotranslational mitochondrial protein importCellOctober 2, 2025Zikun Zhu, Saurav Mallik, Taylor A. Stevens, Riming Huang, Emmanuel D. Levy, Shu-ou Shan"T4 RNA Ligase 2, truncated K227Q",NEB,M0351L Rec J Exonuclease,epicentre,RJ411250 5’ Deadenylase,NEB,M0331S 5’ DNA Adenylation Kit,NEB,E2610S Superscript™ III Reverse Transcriptase,Invitrogen,18080044 Human Ribo-Seq 12 rxn Probes,Galen molecular,dp-R012-000042 Pierce Streptavidin Magnetic Beads

(5) Protocol for Disome-seq to identify transcriptome-wide ribosome collisions in yeast cellsSTAR ProtocolsSeptember 19, 2025Pedro H. Ayres-Galhardo, James Marks, Sezen Meydanpolyacrylamide gel, 12 well",Bio-Rad,3450091 Glycoblue,Thermo Fisher Scientific,AM9516 Adenylation mix,NEB,E2610 NEBuffer 1,NEB,B7001S Oligo Clean & Concentrator,Zymo,D4061 T4 PNK,NEB,M0201L T4 RNA ligase 2 truncated K227Q

(6) Lysosomal RNA profiling reveals targeting of specific types of RNAs for degradationbioRxivSeptember 16, 2025G. Jordan Ray, Eleonora Nardini, Heather R. Keys, Daniel H. Lin, David M. Sabatini, David P. Bartelcleaned up using MinElute reaction cleanup kits (Qiagen 28204). Then cDNA was ligated to adenylated (NEB E2610S) R1R DNA (/5Phos/NNNNNNNNNNGATCGTCGGACTGTAGAACTCTGAACGTGTAG/3SpC3/) using Thermostable 5’ AppDNA/RNA ligase

(7) Simultaneous Capture of Chromatin-Associated RNA and Global RNA–RNA Interactions With Reduced Input RequirementsBIO-PROTOCOLSeptember 5, 2025Cheng Ding, Guoting Chen, Shiping Luan, Yuanyuan Gong, Cuilin Gui, Chen Yang, Zihe Xiang, Junjie Du, Mohamed F. Foda, Jiapei Yan, et al.(Thermo Fisher Scientific, catalog number: EK0032) 23. 5′ DNA Adenylation kit (NEB, catalog number: E2610L) 24. T4 RNA ligase 2, truncated KQ (200 U/μL) (NEB, catalog number: M0373L) 25. T4 RNA ligase reaction buffer

(8) Identification of translation events that drive nonsense-mediated mRNA decay reveals functional roles for noncoding RNAsbioRxivAugust 17, 2025David J. Young, Yuejun Wang, Nicholas R. Guydoshligase 2 (K227Q) (NEB; M0351L). The linkers were pre-adenylated using a 5’ DNA adenylation kit (NEB; E2610L). Unligated linker was removed from the ligation reaction by addition of 5’ deadenylase (NEB; M0331S)

(9) Sequence and parent-of-origin dependent m6A contribute to allele-specific gene expressionThe EMBO JournalJune 3, 2025Ying Zhang, Ze-Yu Zhang, Hong-Xuan Chen, Chao Liu, Biao-Di Liu, Ye-Lin Lan, Ying-Yuan Xie, Tao Chen, Shaobo Chen, Guihai Feng, et al.Synthesized 3′-adapter encoded with 6-nt barcode was pre-adenylated using 5′ DNA Adenylation Kit (NEB, E2610S). For each sample from RNA preparation step, 200 ng mRNA/ribo-depleted RNA of each sample was fragmented

(10) METTL3 inhibits primed-to-naïve transition of pluripotent stem cells through m6A-YTHDF2-pluripotency/Gstp1 mRNA degradation axisCell RegenMay 27, 2025Sa Li, Jiajie Hao, Guangliang Hong, Hongzhi Dong, He Liu, Lingmei Jin, Zhihao Zhang, Haoyu Wu, Mingli Hu, Rujin Huang, et al.T4 Polynucleotide Kinase (Thermo Fisher, EK0031). The purified RNA fragments were ligated to adenylated 3’ adapters (NEB, E2610) using T4 RNA ligase 2 (NEB, M0373), with excess adapters removed by Lambda Exonuclease (NEB, M0262).

(11) DragonRNA: Generality of DNA-primed RNA-extension activities by DNA-directed RNA polymerasesNucleic Acids ResApril 8, 2025Emily Greenwald, Drew Galls, Joon Park, Nimit Jain, Stephen B Montgomery, Bijoyita Roy, Y Whitney Yin, Andrew Z Fire[39]. Oligo preparations The 3′ adapter, AF-DG-35 was adenylated using the 5′ DNA adenylation kit (NEB E2610), according to manufacturer’s protocol. The adenylation reaction was purified with the Oligo Clean & Concentrator

(12) m6A modification regulates cell proliferation via reprogramming the balance between glycolysis and pentose phosphate pathwayCommun BiolMarch 26, 2025Jian-Fei Xi, Biao-Di Liu, Guo-Run Tang, Ze-Hui Ren, Hong-Xuan Chen, Ye-Lin Lan, Feng Yin, Zigang Li, Wei-Sheng Cheng, Jinkai Wang, et al.T4 Polynucleotide Kinase (Thermo Fisher, EK0031). These sample were then ligated to adenylated (NEB, E2610) barcoded 3′ adapters using T4 RNA ligase 2, truncated KQ (NEB, M0373). The excess adapters were subsequently

(13) Simultaneous profiling of chromatin-associated RNA at targeted DNA loci and RNA-RNA Interactions through TaDRIM-seqNat CommunFebruary 10, 2025Cheng Ding, Guoting Chen, Shiping Luan, Runxin Gao, Yudong Fan, Ying Zhang, Xiaoting Wang, Guoliang Li, Mohamed F. Foda, Jiapei Yan, et al.5′-[5Phos]GTCAGC/iBIOdT/CAAGTATCGAG-3′. The 5′App ends of the forward strand were treated using a DNA 5′ Adenylation Kit (NEB; E2610L). The isolated nuclei were washed twice with 500 µl 1× wash buffer B and resuspended in 400 µl of RNA

(14) Bacteroides expand the functional versatility of a conserved transcription factor and transcribed DNA to program capsule diversityNature CommunicationsDecember 30, 2024Jason Saba, Katia Flores, Bailey Marshall, Michael D. Engstrom, Yikai Peng, Atharv S. Garje, Laurie E. Comstock, Robert Landicknascent transcript 3′ end ligation was adenylated using components from a NEB 5´ DNA Adenylation kit (E2610; 6 µM DNA linker [RL15032], 80 µM ATP, 6 µM Mth RNA ligase, and 1× Adenylation Reaction Buffer). The

(15) Protocol for mapping RNA G-quadruplex for chromatin-bound RNA using d-rG4-seqSTAR ProtocolsDecember 20, 2024Yong Woo Lee, Vered Levy, Jeannie T. Leeassays,Critical commercial assays,Critical commercial assays ,, 5′ DNA Adenylation Kit,New England Biolabs,Cat#E2610S Oligotex Direct mRNA kit,QIAGEN,Cat# 70022 Oligo Clean & Concentrator,Zymo,Cat#D4060 ZR small-RNA

(16) Nucleotidyltransferase toxin MenT extends aminoacyl acceptor ends of serine tRNAs to control Mycobacterium tuberculosis growthNature CommunicationsNovember 6, 2024Xibing Xu, Roland Barriot, Bertille Voisin, Tom J. Arrowsmith, Ben Usher, Claude Gutierrez, Xue Han, Carine Pagès, Peter Redder, Tim R. Blower, et al.sufficiently by this near-neutral pH. The 3p-v4 oligo was 5′ adenylated using a 5′ DNA Adenylation Kit (E2610S, NEB) according to the manufacturer’s protocol. To construct the library, RNA samples were ligated to

(17) RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues, cancer cells, and macrophagesMolecular CellSeptember 19, 2024Teodorus Theo Susanto, Victoria Hung, Andrew G. Levine, Yuxiang Chen, Craig H. Kerr, Yongjin Yoo, Juan A. Oses-Prieto, Lisa Fromm, Zijian Zhang, Travis C. Lantz, et al.E7760L Direct-zol RNA Microprep Kits,Zymo Research,Cat# R2060 5´ DNA Adenylation Kit,New England Biolabs,Cat# E2610S Oligo Clean & Concentrator,Zymo Research,Cat# D4060 CircLigase ssDNA Ligase,Biosearch Technologies,Cat#

(18) Endonucleolytic RNA cleavage drives changes in gene expression during the innate immune responseCell ReportsJune 25, 2024Agnes Karasik, Hernan A. Lorenzi, Andrew V. DePass, Nicholas R. Guydoshhuman,siTools,dp-K012-000042 CircLigase ssDNA ligase kit,Biosearch Technologies,CL4115K 5’ DNA adenylation kit,NEB,E2610L Oligo clean & concentrator kit,Zymo Research,D4060 Agilent RNA ScreenTape components,Agilent,5067-55765067-55775067-5578 Agilent

(19) PRO‐seq: Precise Mapping of Engaged RNA Pol II at Single‐Nucleotide ResolutionCurrent ProtocolsDecember 27, 2023Claudia A. Mimoso, Seth R. Goldman(See Table S1, Integrated DNA Technologies) 5′ DNA adenylation kit (New England BioLabs, cat. no. #E2610S) Sodium Acetate pH 5.5, RNase-free (ThermoFisher Scientific, cat. no. AM9740) Glycoblue (ThermoFisher

(20) RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophagesbioRxivDecember 7, 2023Teodorus Theo Susanto, Victoria Hung, Andrew G. Levine, Craig H. Kerr, Yongjin Yoo, Yuxiang Chen, Juan A. Oses-Prieto, Lisa Fromm, Kotaro Fujii, Marius Wernig, et al.as per the published protocol. RNA linkers were pre-adenylated beforehand using Mth RNA ligase (NEB, E2610S). Unreacted linker was deadenylated and digested as per the published protocol. The barcoded RFPS were

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5 DNA Adenylation Kit

5 DNA Adenylation Kit from New England Biolabs

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5 DNA Adenylation Kit from New England Biolabs

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