Fig 1: Effect of HFD and polyphenols on leptinemia, adiponectinemia and plasma CRP levels. Mice were exposed to a normal diet (ND) or a high-fat diet (HFD) in the presence or not of A. borbonica polyphenolic extract (HFD + A.b; 35 mg per kg body weight) or caffeic acid (HFD + CA; 35 mg per kg body weight) for 12 weeks before a cerebral ischemia-reperfusion. After a cerebral ischemia of 90 min and 20 h of reperfusion, (A) leptinemia, (B) adiponectinemia and (C) plasma CRP levels were determined by ELISA kits. Data were expressed as means ± SEM of n ≥ 5. * p < 0.05 and *** p < 0.005 vs. ND. $$ p < 0.01 and $$$ p < 0.005 vs. HFD.
Fig 2: Elevated inflammatory response in cachectic i.v.‐shIFIT2 mice. (A) Human and (B) murine cytokine levels in sera from control (n = 4), s.c.‐shIFIT2 (n = 6), i.v.‐shCTRL (n = 7) and i.v.‐shIFIT2 mice (n = 7). (C) Serum CRP and (D) serum albumin levels in control, s.c.‐shIFIT2, i.v.‐shCTRL and i.v.‐shIFIT2 mice. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the control group. # P < 0.05 compared with the s.c.‐shIFIT2 group. † P < 0.05, and †† P < 0.01 compared with the i.v.‐shCTRL group.
Fig 3: Relative abundance of (A) C-reactive protein (CRP) and (B) the pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNFα) in C57BL/6J mouse blood serum after receiving dietary aluminum sulfate in their food and drinking water (ad libitum) for 0 (control), 1, 3 and 5 months; as determined by ELISA (see text); N=3 to 5 determinations for each inflammatory marker in each mouse sampled; N=3 mice were analyzed per group; in (A) a dashed horizontal line at 2.0 for CRP; and in (B) a dashed horizontal line at 5.0 (for IL-6 and TNFα) is included for ease of comparison; *p<0.05; **p<0.01 (ANOVA).
Fig 4: CRP was elevated in DR4tgLdlr−/−mice fed a HFHC diet. Sera at day 100 were tested for CRP (μg/mL) in mice fed a RD (grey; n = 12) or HFHC (black; n = 12) diet (male and female mice are represented by triangles and circles, respectively). Data are reported as median with 95% CI. ***p = 0.0009 by Two-way ANOVA with Bonferroni correction.
Fig 5: The effect of the antiPCSK9 vaccine on the serum level of hs-CRP in albino mice. The sham group involved non-treated mice, the CFA group involved the CFA-treated mice, and the vaccine group involved mice who after vaccination were treated with the CFA. Pooling of samples was performed to obtain sufficient sample volume for assay, when needed. Data are expressed as mean ± SD (10 mice per group). Statistical differences at a p-value less than 0.05 were considered to be significant
Supplier Page from Abcam for Mouse C Reactive Protein ELISA Kit (PTX1)