Fig 1: Effect of HFD and polyphenols on leptinemia, adiponectinemia and plasma CRP levels. Mice were exposed to a normal diet (ND) or a high-fat diet (HFD) in the presence or not of A. borbonica polyphenolic extract (HFD + A.b; 35 mg per kg body weight) or caffeic acid (HFD + CA; 35 mg per kg body weight) for 12 weeks before a cerebral ischemia-reperfusion. After a cerebral ischemia of 90 min and 20 h of reperfusion, (A) leptinemia, (B) adiponectinemia and (C) plasma CRP levels were determined by ELISA kits. Data were expressed as means ± SEM of n = 5. * p < 0.05 and *** p < 0.005 vs. ND. $$ p < 0.01 and $$$ p < 0.005 vs. HFD.
Fig 2: Elevated inflammatory response in cachectic i.v.-shIFIT2 mice. (A) Human and (B) murine cytokine levels in sera from control (n = 4), s.c.-shIFIT2 (n = 6), i.v.-shCTRL (n = 7) and i.v.-shIFIT2 mice (n = 7). (C) Serum CRP and (D) serum albumin levels in control, s.c.-shIFIT2, i.v.-shCTRL and i.v.-shIFIT2 mice. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the control group. # P < 0.05 compared with the s.c.-shIFIT2 group. † P < 0.05, and †† P < 0.01 compared with the i.v.-shCTRL group.
Fig 3: Relative abundance of (A) C-reactive protein (CRP) and (B) the pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNFa) in C57BL/6J mouse blood serum after receiving dietary aluminum sulfate in their food and drinking water (ad libitum) for 0 (control), 1, 3 and 5 months; as determined by ELISA (see text); N=3 to 5 determinations for each inflammatory marker in each mouse sampled; N=3 mice were analyzed per group; in (A) a dashed horizontal line at 2.0 for CRP; and in (B) a dashed horizontal line at 5.0 (for IL-6 and TNFa) is included for ease of comparison; *p<0.05; **p<0.01 (ANOVA).
Fig 4: CRP was elevated in DR4tgLdlr-/-mice fed a HFHC diet. Sera at day 100 were tested for CRP (µg/mL) in mice fed a RD (grey; n = 12) or HFHC (black; n = 12) diet (male and female mice are represented by triangles and circles, respectively). Data are reported as median with 95% CI. ***p = 0.0009 by Two-way ANOVA with Bonferroni correction.
Supplier Page from Abcam for Mouse C Reactive Protein ELISA Kit (PTX1)