Description
Product Characteristics: The AssayMax™ Human Interferon-gamma ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of IFN-gamma in human plasma, serum, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human IFN-gamma in approximately 5 hours. A polyclonal antibody specific for human IFN- gamma has been pre-coated onto a 96-well microplate with removable strips. IFN-gamma in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human IFN- gamma, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Target Information: This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]