ROS/Superoxide Detection Assay Kit (Cell-based) from Abcam

Product Specs
ItemROS/Superoxide Detection Assay Kit (Cell-based)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab139476
Quantity1 Kit
Sample TypeAdherent cells, Suspension cells
Time0h 90m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Long-Term Static Cultivation Alters Lipid Metabolism and Bioenergetic Capacity in A549 CellsInt J Mol SciApril 10, 2026Ivana Ďurišová, Lucia Šofranková, Aleš Kvasnička, Miroslav Baláž, Ivana Fábryová, David Friedecký, Mária BalážováDetection Intracellular ROS levels in A549 cells were measured using the ROS/Superoxide Detection Assay Kit (Cell-based, ab139476, Abcam, Cambridge, UK) according to the manufacturer’s instructions. Briefly, cells were cultured for

(2) Short-term cigarette smoke exposure aggravates oxidative stress and airway inflammation induced by lipopolysaccharidesFrontiers in PhysiologyMarch 19, 2026Ziyao Liang, Zhihang Liu, Wenchao Pan, Long Fan, Jingyu Quan, Lin Lin, Lei Wu, Xuhua YuFor superoxide detection, the Cellular ROS/Superoxide Detection Assay Kit (Abcam, United States, cat# ab139476) was used according to the manufacturer’s instructions. After staining, cells were stimulated with phorbol 12,13-dibutyrate

(3) Age-related adiponectin resistance in human skeletal muscle dysfunction: in vivo and in vitro evidenceJournal of Translational MedicineMarch 5, 2026Surina Surina, Lucia Scisciola, Manuela Giovanna Basilicata, Ada Pesapane, Rosaria Anna Fontanella, Nunzia Balzano, Alberta Maria Maddalena Palazzo, Asad Zia, Giovanni Tortorella, Zeeshan Ulfat, et al.TGATCCACATC-3′). ROS/superoxide detection assay Intracellular ROS levels were measured using an ROS detection kit (Abcam, ab139476). The staining protocol followed the manufacturer’s instructions for the ROS/Superoxide Detection Assay

(4) Targeting TRAF3IP2 disrupts cellular energetics through inhibition of NAMPT in triple negative breast cancerSci RepDecember 23, 2025Kurtis Willingham, Amin Izadpanah, Yasmine Rashad, Antonia Reilich, Fatemeh Daneshimehr, Steven Braun, Eckhard U. Alt, Reza Izadpanahquantities in a 96 well plate. ROS production was assessed using a ROS/Superoxide Detection Assay Kit (cat# ab139476, Abcam, Cambridge, UK), following protocol for incubation of treatment types and positive control (SCR

(5) Attenuation of ATM signaling by ROS delays replicative senescence at physiological oxygenMolecular CellDecember 18, 2025Alexander J. Stuart, Kaori K. Takai, Railia R. Gabbasova, Henry Sanford, Ekaterina V. Vinogradova, Titia de Lange,R&D Systems,Cat#4250–050-K MagAttract HMW Kit,Qiagen,Cat#67563 ROS/Superoxide Detection Kit,Abcam,Cat#ab139476 DC Protein Assay Reagent A,Bio-rad,Cat#5000113 DC Protein Assay Reagent B,Bio-rad,Cat#: 5000114 Deposited

(6) Synergistic toxicity of PFAS and microplastic mixtures across five human cell linesEnvironmental PollutionDecember 1, 2025Mia Sands, Arshveer Sachdeva, Laura Bukavina, Joseph Irudayarajproduction in five cell lines were assessed using the ROS/Superoxide Detection Assay Kit (Cell-based) (ab139476; Abcam, Waltham, MA, USA). Following treatment, cells were washed with the wash buffer provided in the

(7) Piceatannol and Pumpkin Seed Oil Synergistically Promote Apoptosis of PC3 Prostate Cancer Cells via Intrinsic Mitochondrial PathwayACS OmegaNovember 11, 2025Mohammed Z. Nasrullah, Lenah S. Binmahfouz, Amina M. Bagher, Rawan H. Hareeri, Hawazen H. Bin-Melaih, Osama A. Abuzinadah, Azizah M. Malebari, Ashraf B. Abdel-Naim, Thikryat Neamatallahassay kit was used in accordance with the manufacturer’s guidelines to determine ROS in PC3 cells (ab139476, Abcam, Cambridge, MA, USA). The method used depends on the real-time monitoring of ROS and the production

(8) Novel Anthraquinone Chlorination Contributes to Pigmentation and ATP Formation in Thermomyces dupontiiMicrob BiotechnolOctober 22, 2025Donglou Wang, Gang Dai, Jiangbo He, Huiwen Si, Chunhua Liao, Wenjie Wang, Zhangxin Zuo, Shuhong Li, Xuemei Niureactive oxygen species (ROS) and superoxide were measured using the ROS/Superoxide Detection Assay Kit (#ab139476, Abcam) following the manufacturer's instructions. Briefly, 20 mg of mycelia were harvested in a 2 mL

(9) Na2SeO3 Exposure Inhibits Locomotion and Reproduction via Oxidative Stress Mechanism in Bioindicators C. elegans and Acrobeloides sp.ToxicsOctober 13, 2025Fan Jin, Jiaping Song, Shanshan Niu, Xuebin Yin(GSH-Px) activity (cat. no. ab102530; Abcam, Cambridge, UK), reactive oxygen species (ROS) level (cat. no. ab139476; Abcam, Cambridge, UK) and malondialdehyde (MDA) content (cat. no. ab118970; Abcam, Cambridge, UK). 2.5.

(10) Insights from dual-platform metabolomics on durian flowers: An alternative source of procyanidins from agricultural waste with bioactivities☆Food Chem XJuly 1, 2025Supakorn Potijun, Nattaya Pattarapipatkul, Pitchakorn Boonma, Putthamas Pewlong, Intira Pathtubtim, Thanchanok Muangman, Bunyarit Meksiriporn, Hubert Schaller, Supaart Sirikantaramasprocured from Thermo Fisher Scientific (Seoul, Korea), and the Cellular ROS/Superoxide Detection Assay (ab139476), Human TNF alpha ELISA (ab181421), and Human IL-6 ELISA (ab178013) kits were procured from Abcam (Cambridge,

(11) Small-molecule inhibitors of 6-phosphofructo-1-kinase simultaneously suppress lactate and superoxide generation in cancer cellsPLoS OneMay 21, 2025Samo Lešnik, Janez Konc, Tina Vodopivec, Katja Čamernik, Urška Karolina Potokar, Matic LegišaSOX detection reagent (Orange) were added as specified in the Cellular ROS/SOX Detection Assay Kit (ab139476 Abcam, Cambridge, UK). For measuring superoxide Cellular Superoxide Detection Assay Kit (ab139477 Abcam,

(12) In Vitro, Ex Vivo, Instrumental, and Clinical Assessment of a Novel Anti-aging Serum Targeting Oxidative StressJ Cosmet DermatolApril 3, 2025Sayantani Goswami, Jin Namkoong, Marwa El Hajoui, Ewelina Lesniak, Joanna Wuproduction was assessed in‐tubo using an adapted version of the ROS/Superoxide Detection Assay Kit (#ab139476, Abcam, MA, USA). Diluted serum (0.02% dilution of either serum in ROS/Superoxide Detection Assay Kit

(13) Single-cell analysis of neoplastic plasma cells identifies myeloma pathobiology mediators and potential targetsCell Reports MedicineFebruary 18, 2025Luz Yurany Moreno Rueda, Hua Wang, Keiko Akagi, Minghao Dang, Amishi Vora, Li Qin, Hans C. Lee, Krina K. Patel, Pei Lin, David E. Mery, et al.Vybrant™ DyeCycle™ Violet Stain,Thermo Fisher Scientific,V35003 ROS/Superoxide Detection Assay Kit (Cell-based),Abcam,ab139476 Pierce™ Magnetic ChIP Kit,Thermo Fisher Scientific,26157 Nano-Glo® Dual-Luciferase Reporter Assay System

(14) Inhibition of Glutamate‐to‐Glutathione Flux Promotes Tumor Antigen Presentation in Colorectal Cancer CellsAdvanced ScienceOctober 31, 2024Tao Yu, Kevin Van der Jeught, Haiqi Zhu, Zhuolong Zhou, Samantha Sharma, Sheng Liu, Haniyeh Eyvani, Ka Man So, Naresh Singh, Jia Wang, et al.ab112132), ROS levels were measured using the Cellular ROS/Superoxide Detection Assay Kit (Abcam, Cat#: ab139476) according to the manufacturer's instructions. Briefly, the cells were collected and incubated with

(15) Thermophilic fungus uses anthraquinones to modulate ferrous excretion, sterol‐mediated endocytosis, and iron storage in response to cold stressMicrobial BiotechnologySeptember 1, 2024Shuhong Li, Donglou Wang, Jiangbo He, Chunhua Liao, Zhangxin Zuo, Shenghong Li, Xuemei NiuDojindo, Japan) according to the above method. ROS/superoxide assay ROS/Superoxide Detection Assay Kit (#ab139476, Abcam, Britain) was used to directly monitor ROS and Superoxide in mycelia. Twenty mg mycelia was harvested

(16) The M2 Protein of the Influenza A Virus Interacts with PEX19 to Facilitate Virus Replication by Disrupting the Function of PeroxisomeVirusesAugust 16, 2024Liu T, Liang L, Zhao P, Lin W, Zhuang Y, Jiang L, Chen H, Li C.(H1N1)-infected or PEX19 siRNA1-treated A549 cells and HEK293T cells loaded with ROS detection reagents (ab139476; Abcam) at 24 h p.i. or 24 h post transfection. 2.13. Confocal Microscopy A549 cells of approximately

(17) Mechanism and rational combinations with GP‐2250, a novel oxathiazine derivative, in ovarian cancerCancer MedicineAugust 8, 2024Mark S. Kim, Deanna Glassman, Katelyn F. Handley, Adrian Lankenau Ahumada, Nicholas B. Jennings, Emine Bayraktar, Katherine Foster, Robiya Joseph, Sanghoon Lee, Robert L. Coleman, et al.oxygen species (ROS) and peroxide levels were measured using a cell‐based fluorogenic assay kit. (Abcam; ab139476). 2.11 ATP assay A total of 2.5 × 106 ovarian cancer cells were incubated overnight onto 100 mm tissue culture

(18) Effect of extracellular matrix stiffness on efficacy of Dapagliflozin for diabetic cardiomyopathyCardiovascular diabetologyJuly 24, 2024Zhu T, Ye Z, Song J, Zhang J, Zhao Y, Xu F, Wang J, Huang X, Gao B, Li F.ROS levels The measurement of intracellular ROS levels was performed with a ROS detection assay kit (ab139476, Abcam, Cambridge, MA, USA). The H9c2 cells were incubated with the fluorescent dye at 37 °C for 30

(19) Bitter Taste Receptor 46 (hTAS2R46) Protects Monocytes/Macrophages from Oxidative StressInternational Journal of Molecular SciencesJuly 3, 2024Maria Talmon, Lara Camillo, Ilaria Vietti, Federica Pollastro, Luigia Grazia Fresupercentage of cells producing ROS/RNS using the Cellular ROS/Superoxide Detection Assay Kit (Abcam ab139476, Cambridge, UK) according to the manufacturer’s instructions. Data were collected and analysed through

(20) Exosome-coated oxygen nanobubble-laden hydrogel augments intracellular delivery of exosomes for enhanced wound healingNature communicationsApril 23, 2024Han X, Saengow C, Ju L, Ren W, Ewoldt RH, Irudayaraj J.except Goat anti-Rabbit IgG (H + L) Secondary Antibody (1:50). ROS/Superoxide detection assay kit (ab139476), calcein AM (ab141420), DAPI (ab228549) were purchased from Abcam. Human mesenchymal stem cell marker antibody panel

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8/21/2023ROS Detection Assay Kit From Abcam Shivali RawatIn my study, proteostasis stress resulted in the aggregation of mitochondrial proteins inside cells. To investigate the effect on mitochondrial function, I experimented to test levels of ROS under ... Read Review

ROS/Superoxide Detection Assay Kit (Cell-based) from Abcam

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ROS/Superoxide Detection Assay Kit (Cell-based) from Abcam

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