Lamina Propria Dissociation Kit, mouse 130-097-410 from Miltenyi Biotec

(1) Interferon lambda signaling to maternal dendritic cells protects against congenital Zika virus infectionbioRxivFebruary 1, 2026Margaret R. Dedloff, Thomas R. Reinhardt, Tessa-Jonne F. Ropp, Joel Rivera-Cardona, Celeste R. Robles, Rebecca L. Casazza, J. Ashley Ezzell, Michelle S. Itano, Helen M. LazearGentleMACS (Miltenyi #130–134-029) and enzymes from the mouse lamina propria dissociation kit (Miltenyi #130–097-410). Uterus samples were passed through 70μm and 40μm filters, centrifuged at 350 × g for 5 minutes and

(2) Sustained in situ protein production and release in the mammalian gut by an engineered bacteriophageNature BiotechnologyJanuary 1, 2026Zachary R. Baker, Yao Zhang, Haiyan Zhang, Hollyn C. Franklin, Priscila B. S. Serpa, Teresa Southard, Liwu Li, Bryan B. Hsuincubated in the digestion solution provided by Lamina Propria Dissociation Kit (Miltenyi Biotec, Catalog # 130-097-410) according to the manufacturer’s instruction. The lamina propria cells were resuspended in the FACS

(3) Cathelicidin regulates goblet cell mucus secretion and mucus-associated proteins in Citrobacter rodentium -induced colitisGut MicrobesDecember 31, 2025Niloofar Mirzadzare, Graham A. D. Blyth, Rita Hannawayya, Karina M. Cirone, Geeta Kilari, Priyoshi Lahiri, Yi Lin Tan, Aydin Ivan Herik, Yongpeng Fu, Hayley Gorman, et al.rodentium-challenged mice at the peak of infection. Lamnia propria cells were isolated using a dissociation kit (130–097–410 Miltenyi Biotech) and stained with eBioscience™ Fixable Viability Dye eFluor™ 780, Spark Blue™ 550 anti-mouse

(4) Gut microbial dysbiosis activates the classical complement pathway in a short-term morphine treatment modelGut Microbes ReportsDecember 31, 2025Nicolas Vitari, Richa Jalodia, Junyi Tao, Udhghatri Kolli, Salma Singh, Lily V. Rodriguez, Umakant Sharma, Sabita Roylamina propria were isolated using the Lamina Propria Dissociation Kit (Miltenyi Biotec; catalog no. 130–097–410) according to the manufacturer’s recommendations. Following isolation, immune cells were washed with

(5) Spatially resolved single-cell transcriptome analysis of murine Salmonella infection reveals the role of distal colonocytes in the inflammatory responseGut MicrobesDecember 31, 2025Dan Xu, Ruifen Zhang, Shanshan Li, Can Guo, Chenglin Guan, Xiang Li, Mengyao Guo, Xin Xu, Yaxin Liu, Chenyi Mao, et al.lamina propria cells were collected by gentle dissociation using a Lamina Propria Dissociation Kit (mouse, #130-097-410; Miltenyi Biotec) for 15 min. The remaining tissue was then subjected to further harsher dissociation

(6) Perioperative anti-α4β7 Integrin Blockade Alters Wound Cell Infiltrate but Not the Functional Outcome of Ileocecal Anastomoses in Mice With Crohn's-like IleitisInflammatory bowel diseasesDecember 1, 2025Berlin P, Fischer A, Bergmann-Ewert W, Kordt M, Schafmayer C, Lamprecht G, Reiner J, Witte MB.lamina propria dissociation kit according to the manufacturer’s instructions (Miltenyi Biotec; Catalog No. 130-097-410). The tissue was then predigested in HBSS without Ca2+ and Mg2+, containing 5 mM EDTA, 5% fetal bovine

(7) Entamoeba muris provides a mouse model for amoebic colitis pathology characterized by T cell associated colonizationScientific ReportsNovember 14, 2025Tae Hun Ha, Sang Hyeok Seok, Heok won An, Jong-Wan Kwon, Gyeong Min Yoon, Yu Jin Lee, Na Yun Lee, Hyeon Ah Kim, Won Gi Yoo, Su-Hyung Lee, et al.subjected to enzymatic dissociation using the Lamina Propria Dissociation Kit (mouse; Miltenyi Biotec, Cat. 130−097-410) according to the manufacturer’s protocol to obtain single-cell suspensions. Immune cells were stained

(8) Lack of phospho-eIF4E worsens experimental colitis by inhibiting Treg suppressive activityJournal of InflammationNovember 6, 2025François Santinon, Theodoros Papadopoulos, Meagan-Helen Henderson Berg, Paige McCallum, Madelyn J. Abraham, Christophe Gonçalves, Vrinda Gupta, Natascha Gagnon, Noah Wirasinghe, Hsiang Chou, et al.Colonic immune cells were extracted using the lamina propria dissociation kit from Miltenyi Biotec (130-097-410) and the GentleMACS dissociator from Miltenyi Biotec (130-093-235). Flow cytometry For general flow cytometric analysis

(9) miR147 promotes mucosal integrity and healing in intestinal inflammationJCI InsightOctober 22, 2025Agnieszka K. Czopik, Arash Dabiri, Chia-Hao Tung, Victoria Vaughn, Xiangsheng Huang, Jinlian Wang, Hui Li, Nicolas F. Moreno, Natalia V. Piwko, Katherine Figarella, et al.LPLs were isolated from colons of 8- to 12-week-old WT mice using the Lamina Propria Dissociation Kit (130-097-410, Miltenyi Biotec) per the manufacturer’s instructions. Cell pellets were lysed in QIAzol (79306, QIAGEN)

(10) A simple and efficient method for the robust expansion of human ILC2s to prevent acute GVHD∗†Blood AdvancesOctober 14, 2025Danny W. Bruce, Sonia J. Laurie, Yan Xing, Heather E. Stefanski, Keli L. Hippen, Alec D. Wilkinson, Mark G. Woodcock, James M. Coghill, Karen Peterson McKinnon, Bruce R. Blazar, et al.established using Miltenyi Lung Dissociation kit (130-095-927) and Miltenyi Lamina Propria Dissociation kit (130-097-410) as per manufacturer’s instruction; liver and spleen cells were isolated by dissociation through 70-μm

(11) Amyloid-β-driven Alzheimer's disease reshapes the colonic immune system in miceCell reportsSeptember 23, 2025Makhijani P, Valentino TR, Manwaring-Mueller M, Emani R, Mu WC, Aguirre CG, Tan CR, Rane A, Wilson KA, Kifle A, et al.7.5 inches in length. Colons were processed using a lamina propria dissociation kit (Miltenyi Biotec, 130–097-410) with the gentleMACS Dissociator (Miltenyi Biotec, 130–093-235). Prior to brain collection, mice were

(12) TAGAP restrains myeloid and T cell activation in inflammatory bowel diseaseFrontiers in ImmunologySeptember 22, 2025Rui-Ci Lin, Zhifei ShaoThe IEL and LPL from SI were collected with the lamina propria dissociation kit (Miltenyi Biotec; #130-097-410) according to the manufacturer’s manual. The dissociated IEL and LPL cells were loaded onto a Percoll

(13) Oral Bioavailability of a Noncoding RNA Drug, TY1, That Acts on MacrophagesJ Extracell BiolAugust 14, 2025Shukuro Yamaguchi, Kazutaka Miyamoto, Xaviar M. Jones, Alessandra Ciullo, Kara Tsi, Jessica Anderson, Hiroaki Komuro, Salwa Soussi, Ashley Morris, Diana Kitka, et al.described above. Cells were extracted from intestinal tissues using the Lamina Propria Dissociation Kit (130‐097‐410, Miltenyi) to make a single‐cell suspension according to the manufacturer's protocol with minor modifications.

(14) Ripk1 is critical for preserving effector regulatory T cells and the suppressive transcriptional program in regulatory T cellsCell Death & DifferentiationJuly 22, 2025Carlos Plaza-Sirvent, Hannah Sturm, Maximilian K. Nocke, Fatemeh Ghorbani, Clara Bessen, Marina C. Greweling-Pils, Stefan Floess, Jana Niemz, Jelle Huysentruyt, Peter Tougaard, et al.immune cells and intraepithelial lymphocytes were isolated using Lamina Propria Dissociation Kit, mouse (130-097-410, Miltenyi Biotec) and gentleMACS Octo Dissociator with Heaters (130-096-427, Miltenyi Biotec) following

(15) Secretory IgA dysfunction underlies poor prognosis in Fusobacterium-infected colorectal cancerGut MicrobesJuly 16, 2025Ilseok Choi, Kyung-A Kim, Sang Cheol Kim, Donghwan Park, Ki Taek Nam, Jun Hyung Cha, Seungbyn Baek, Junha Cha, Hye-Yeong Jo, Minsun Jung, et al.(Gibco, USA) and an enzyme mix (enzymes D, R, and A) from the Mouse Lamina Propria Dissociation Kit (Cat# 130–097–410, Miltenyi Biotec, USA). Dissociation was performed at 37°C for 30 min using a gentleMACS Octo Dissociator with heaters

(16) Phosphocreatine Rescues Intestinal Epithelial Metabolic Dysfunction Related to Creatine Kinase Loss and Is Protective in Murine ColitisCellular and Molecular Gastroenterology and HepatologyJune 23, 2025Geetha Bhagavatula, Corey S. Worledge, Ciara Schaepe, Emily M. Murphy, Rane M. Neuhart, J. Scott Lee, Ian Cartwright, Sean P. Colgan, Caroline H.T. Hallproximal colon was isolated, flayed, and digested using Lamina Propria Dissociation kit (Miltenyi Biotec, 130-097-410) with mechanical disruption using the GentleMACS tube (Miltenyi Biotec, 130-096-334). Digested solution

(17) Protocol to study in vivo organ-specific migration of apoptotic splenocytes in mice with tumor and immune checkpoint inhibitor-induced colitisSTAR ProtocJune 20, 2025Lukas M. Braun, Robert Zeiserlamina propria according to manufacturer’s instructions (Lamina Propria Dissociation Kit, Miltenyi, #130-097-410).9.Prepare predigestion and digestion solutions freshly before lamina propria dissociation. CRITICAL:

(18) Alterations of DNA Repair and Immune Infiltration in Radiation-Induced Intestinal InjuryDose ResponseApril 17, 2025Jiale Li, Huanteng Zhang, Xiaoxiao Jia, Jiebing Guan, Xumin Zong, Shiqi Li, Xinran Lu, Ningning He, Huijuan Song, Kaihua Ji, et al.The small intestinal lamina propria suspension was prepared using the lamina propria isolation kit (130-097-410, Miltenyi Biotec, Germany) according to the manufacturer’s protocol. The following antibodies were used

(19) Microbiota-produced immune regulatory bile acid metabolites control central nervous system autoimmunityCell Reports MedicineApril 15, 2025Martina Antonini Cencicchio, Federico Montini, Vittoria Palmieri, Luca Massimino, Marta Lo Conte, Annamaria Finardi, Alessandra Mandelli, Francesco Asnicar, Radmila Pavlovic, Denise Drago, et al.Mix,Life Technologies,Cat# 4472908; "Lamina Propria Dissociation Kit, mouse",Miltenyi Biotec,Cat# 130-097-410; Leukocytes Activation Cocktail with BD GolgiPlug™,BD Bioscience,Cat# 550583; Mouse GM-CSF,Miltenyi Biotec

(20) The Roles of Ninjurin1 and Estrogen in Modulating Azoxymethane/Dextran Sodium Sulfate-Induced Colitis-Associated Colorectal Cancer in Male MiceCancer Research and TreatmentJanuary 13, 2025Chin-Hee Song, Nayoung Kim, Ryoung Hee Nam, Jae Young Jang, Eun Hye Kim, Sungchan Ha, Eun Shin, Ha-Na Lee, Hoon Choi, Kyu-Won Kim, et al.tissue Lamina propria was extracted from the whole colon using a mouse Lamina Propria Dissociation Kit (#130-097-410), gentleMACS C tube (#130-096-334), and gentleMACS Octo Dissociator with Heaters (#130-096-427), all

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Lamina Propria Dissociation Kit, mouse (for 50 preparations)

Lamina Propria Dissociation Kit, mouse from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Lamina Propria Dissociation Kit, mouse from Miltenyi Biotec

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