Treg Expansion Kit, mouse 130-095-925 from Miltenyi Biotec

(1) Ripk1 is critical for preserving effector regulatory T cells and the suppressive transcriptional program in regulatory T cellsCell Death & DifferentiationJuly 22, 2025Carlos Plaza-Sirvent, Hannah Sturm, Maximilian K. Nocke, Fatemeh Ghorbani, Clara Bessen, Marina C. Greweling-Pils, Stefan Floess, Jana Niemz, Jelle Huysentruyt, Peter Tougaard, et al.sodium pyruvate (all from Life Technologies). For Treg cell activation, Treg Expansion Kit, mouse (130-095-925, Miltenyi) was used in the presence of 100 ng/ml murine IL-2 (402-ML, R&D). rmTNF (12343014, Immunotools)

(2) Succinate drives gut inflammation by promoting FOXP3 degradation through a molecular switchNature ImmunologyJune 2, 2025Hai Wang, Danqing Hu, Yang Cheng, Qiong Gao, Kun Liu, Nikita L. Mani, Amy Y. Tang, Radhika Iyer, Beixue Gao, Leyu Sun, et al.IL-2 (PeproTech, 212–12), and 5.0×104 anti-CD3/anti-CD28 Mouse T-Activator Dynabeads (Miltenyi Biotec, 130-095-925) for 72 h. In some experiments, succinyl-CoA (5 μM, Millipore Sigma, S1129) was added to the cell culture medium

(3) Increased SOAT2 expression in aged regulatory T cells is associated with altered cholesterol metabolism and reduced anti-tumor immunityNat CommunJanuary 13, 2025Mingjiong Zhang, Jiahua Cui, Haoyan Chen, Yifan Cheng, Qiaoyu Chen, Feng Zong, Xiao Lu, Lang Qin, Yu Han, Xingwang Kuai, et al.Genscript) and TGF-β (10 ng/ml) (#Z03431, #Z03411, Genscript). The Treg Expansion Kit (#130-095-345, #130-095-925, Miltenyi Biotec) was applied for Treg multiplication at a bead-to-cell ratio of 4:1 for human or 3:1

(4) Proteobacteria impair anti-tumor immunity in the omentum by consuming arginineCell Host & MicrobeJuly 10, 2024Selene Meza-Perez, Mingyong Liu, Aaron Silva-Sanchez, Casey D. Morrow, Peter G. Eipers, Elliot J. Lefkowitz, Travis Ptacek, Christopher D. Scharer, Alexander F. Rosenberg, Dave D. Hill, et al.assays,Critical commercial assays,Critical commercial assays Treg Expansion Kit protocol,Miltenyi Biotec,130-095-925; RRID:SCR_008984 L-arginine assay kit,Abcam,ab241028; RRID:SCR_012931 CellTrace™ Violet Cell Proliferation Kit

(5) High glucose impairs cognitive function through inducing mitochondrial calcium overload in Treg cellsiScienceJanuary 19, 2024Hui Y, Kuang L, Zhong Y, Tang Y, Xu Z, Zheng T.according to the protocol provided by the Treg Expansion Kit (Miltenyi Biotec 130-095-345; Miltenyi Biotec 130-095-925). For in vitro experiments in Treg cells, high glucose was engendered by raising the glucose 25mM over

(6) OX40L-Armed Oncolytic Virus Boosts T-cell Response and Remodels Tumor Microenvironment for Pancreatic Cancer TreatmentTheranosticsJanuary 1, 2023Liu S, Li F, Ma Q, Du M, Wang H, Zhu Y, Deng L, Gao W, Wang C, Liu Y, et al.and ground into splenocytes, then CD3+ T cells or Tregs were sorted by magnetic beads (130-094-973, 130-095-925, Miltenyi), respectively. The cell culture medium of KPC infected with the oncolytic virus was replaced

(7) A deubiquitination module essential for T reg fitness in the tumor microenvironmentScience AdvancesNovember 25, 2022Elena Montauti, Samuel E. Weinberg, Peng Chu, Shuvam Chaudhuri, Nikita L. Mani, Radhika Iyer, Yuanzhang Zhou, Yusi Zhang, Changhong Liu, Chen Xin, et al.well with 2000 U of IL-2 and CD3/CD28 beads (from a Treg expansion kit, Miltenyi Biotec, catalog no.130-095-925) and added to T cell medium following the manufacturer’s instructions. In vitro iTreg cell TCM and

(8) Targeting of Cdc42 GTPase in regulatory T cells unleashes antitumor T-cell immunityJournal for immunotherapy of cancerNovember 1, 2022Kalim KW, Yang JQ, Wunderlich M, Modur V, Nguyen P, Li Y, Wen T, Davis AK, Verma R, Lu QR, et al.flow cytometry sorting of CD4+YFP+ cells to >99% purity were expanded with Treg cell expansion kit (Miltenyi, 130-095-925) for 3 days, in the presence or absence of 47.6 mM NaHCO3 (to make culture medium of pH 7.60), 3 µM

(9) NAC1 modulates autoimmunity by suppressing regulatory T cell-mediated toleranceScience advancesJuly 1, 2022Yang JM, Ren Y, Kumar A, Xiong X, Das JK, Peng HY, Wang L, Ren X, Zhang Y, Ji C, et al.Treg (no. 130-091-041), T cell activation/expansion kit (no. 130-093-627), and Treg expansion kit (no. 130-095-925) were purchased from Miltenyi Biotec (Auburn, CA). Recombinant mouse TGF-β (no. 763104), IL-1β (no.

(10) IL18 signaling promotes homing of mature Tregs into the thymuseLifeJuly 20, 2020Peligero-Cruz C, Givony T, Sebé-Pedrós A, Dobeš J, Kadouri N, Nevo S, Roncato F, Alon R, Goldfarb Y, Abramson J.b-mercaptoethanol (Gibco 31350–010). In addition, cells were cultured with or without CD3/CD28 beads (Miltenyi 130-095-925; 4:1 ratio cell to bead), and 10 ng/mL or 100 ng/mL recombinant mouse IL18 (Biolegend 767002). Cells

(11) Regulatory T Cells Play a Role in a Subset of Idiopathic Preterm Labor/Birth and Adverse Neonatal OutcomesCell reportsJuly 7, 2020Gomez-Lopez N, Arenas-Hernandez M, Romero R, Miller D, Garcia-Flores V, Leng Y, Xu Y, Galaz J, Hassan SS, Hsu CD, et al.,Miltenyi Biotec,Cat# 130-091-041 "Treg Expansion Kit, mouse (CD3/CD28 MACSiBead particles)",Miltenyi Biotec,Cat# 130-095-925 Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation,"GIBCO, Thermo Fisher Scientific",Cat#

(12) The lineage stability and suppressive program of regulatory T cells require protein O-GlcNAcylationNature communicationsJanuary 21, 2019Liu B, Salgado OC, Singh S, Hippen KL, Maynard JC, Burlingame AL, Ball LE, Blazar BR, Farrar MA, Hogquist KA, et al.Treg cells were expanded according to the Treg Expansion Kit protocol (Miltenyi Biotec, catalog no. 130–095–925). Treg cells were treated with 0.5 μM 4-Hydroxytamoxifen (4-OHT) (Millipore Sigma, catalog no. H7904)

(13) Mitochondrial complex III is essential for suppressive function of regulatory T cellsNatureJanuary 1, 2019Samuel E. Weinberg et al.isolated using FACS sorting and cultured using the Mouse Treg Expansion Kit beads (Miltenyi, Catalog no. 130-095-925) following the manufacture’s protocol. In short, 100,000 CD25+ Treg cells were cultured with 300,000

(14) A Dual Immunotherapy Nanoparticle Improves T-Cell Activation and Cancer ImmunotherapyAdvanced materials (Deerfield Beach, Fla.)June 1, 2018Mi Y, Smith CC, Yang F, Qi Y, Roche KC, Serody JS, Vincent BG, Wang AZ.recombinant murine IL-2, anti-CD3/28 stimulation beads according to manufacturer protocol (Miltenyi, 130-095-925), and 1 µg mL−1 recombinant murine PD-L1 protein (R&D systems, 1019-B7), with media and reagents changed

(15) Adoptive transfer of CD 3 ⁺ T cells and CD 4 ⁺ CD 44 ^high memory T cells induces autoimmune pancreatitis in MRL /MpJ miceJournal of Cellular and Molecular MedicineApril 1, 2018Luise Ehlers, Sarah Rohde, Saleh Ibrahim, Robert Jastermanufacturer's instructions. CD4+CD25+ T cells were cultured using the T reg Expansion Kit, mouse (130‐095‐925; Miltenyi Biotec). Briefly, 2 × 106 cells/mL were dissolved in splenocyte culture medium supplemented

(16) A20 Restrains Thymic Regulatory T Cell DevelopmentJournal of immunology (Baltimore, Md. : 1950)October 1, 2017Fischer JC, Otten V, Kober M, Drees C, Rosenbaum M, Schmickl M, Heidegger S, Beyaert R, van Loo G, Li XC, et al.activated with soluble beads preloaded with CD3 and CD28 Abs according to the manufacturer's instructions (130-095-925; Miltenyi Biotec). At indicated time points after activation (days 4 and 8), intracellular Foxp3 was

(17) Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injuryMolecular Medicine ReportsDecember 1, 2016Yunhu Yu, Fang Cao, Qishan Ran, Xiaochuan Suninstructions. In vitro expansion of Tregs In the present study, we used mouse Treg expansion kit (cat. no. 130-095-925; Miltenyi Biotec GmbH) to conduct in vitro expansion of Treg cells. All procedures were carried out

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Treg Expansion Kit, mouse from Miltenyi Biotec

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Treg Expansion Kit, mouse from Miltenyi Biotec

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