Active Rap1 Detection Kit 8818S from Cell Signaling Technology

Product Specs
ItemActive Rap1 Detection Kit
CompanyCell Signaling Technology
Price $995.00 Inquire
Catalog Number8818S
Quantity1 Kit
Research AreaCytoskeletal Signaling
Molecular Weight21
Detection TargetRap1A
NCBI Full Gene NameRAP1A, member of RAS oncogene family
NCBI Gene AliasesC21KG, G-22K, KREV-1, KREV1, RAP1, SMGP21

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) GDF6 Alleviates Pathological Cardiac Hypertrophy via AMPKα Signaling PathwayBiomedicinesNovember 29, 2025Quan Ren, Zhiwei Wang, Wei RenActivity Assay Kit (#ab139435) were purchased from Abcam (Cambridge, UK). Active Rap1 Detection Kit (#8818) was purchased from Cell Signaling Technology (Danvers, MA, USA). AAV9 carrying short hairpin RNA against

(2) Geranylgeranyl isoprenoids and hepatic Rap1a regulate basal and statin-induced expression of PCSK9Journal of Lipid ResearchMarch 1, 2024Yating Wang, Brea Tinsley, Stefano Spolitu, John A. Zadroga, Heena Agarwal, Amesh K. Sarecha, Lale Ozcanassay Rap1a activity was assayed using Active Rap1 Detection Kit (Cell Signaling Technology, catalog no.: 8818). Liver tissue sample was lysed with lysis/binding/washing buffer containing 2 mM PMSF, 5 μg/ml leupeptin,

(3) Evidence for a PI3-kinase independent pathway in the regulation of Rap1b activation downstream of the P2Y12 receptor in plateletsPlateletsNovember 17, 2022Carol Dangelmaier, Satya P Kunapulinoted. Apyrase and 2-MeSADP, were purchased from Sigma (St. Louis, MO). Active Rap1 detection kit cat#8818 and anti-Akt(Ser473) cat# 4060 were obtained from Cell Signaling (Danvers, MA). Antibody to total Akt

(4) Hepatocyte Rap1a contributes to obesity- and statin-associated hyperglycemiaCell reportsAugust 23, 2022Wang Y, Spolitu S, Zadroga JA, Sarecha AK, Ozcan L.assay Rap1a activity was assayed using Active Rap1 Detection Kit (Cell Signaling Technology, cat # 8818). Cells were washed with cold PBS and lysed with lysis/binding/washing buffer containing 2 mM PMSF,

(5) Gut microbiotaâ€“derived short-chain fatty acids protect against the progression of endometriosisLife Science AllianceDecember 1, 2021Sangappa B Chadchan, Pooja Popli, Chandrasekhar R Ambati, Eric Tycksen, Sang Jun Han, Serdar E Bulun, Nagireddy Putluri, Scott W Biest, Ramakrishna Kommaganiof active RAP1 Active RAP1 (GTP-bound) was detected using an active RAP1 detection kit, Cat, no. #8818; Cell Signaling Technology Inc. All steps were performed according to the manufacturer’s instructions.

(6) Gβγ regulates mitotic Golgi fragmentation and G2/M cell cycle progressionMolecular biology of the cellOctober 1, 2021Rajanala K, Klayman LM, Wedegaertner PB.137635) and anti-Gβ2 (Cat # 108504) antibodies were obtained from Abcam. The antibodies for PKD (Cat # 8818), phospho-PKD/PKCµ (Ser916) (Cat # 2051), tubulin (Cat # 3873), and phospho-histone H3 (Ser10) (Cat

(7) Plexin-B2 facilitates glioblastoma infiltration by modulating cell biomechanicsCommunications biologyJanuary 29, 2021Huang Y, Tejero R, Lee VK, Brusco C, Hannah T, Bertucci TB, Junqueira Alves C, Katsyv I, Kluge M, Foty R, et al.wells were pooled for protein lysis using the Active Rap1 Detection Kit (Cell Signaling Technologies #8818) based on the pulldown of Rap1/2 GTP with the Rap-GTP binding domain of RalGDS. Detection of Rap proteins

(8) Adaptable haemodynamic endothelial cells for organogenesis and tumorigenesisNatureSeptember 1, 2020Palikuqi B, Nguyen DT, Li G, Schreiner R, Pellegata AF, Liu Y, Redmond D, Geng F, Lin Y, Gómez-Salinero JM, et al.ETV2-transduced HUVECs (flat 2D induction stage) was used for the active RAP1 assay (Cell Signaling, 8818S) according to the manufacturer’s guidelines for the kit. In brief, the cells were washed once with PBS

(9) Altered RNA Splicing by Mutant p53 Activates Oncogenic RAS Signaling in Pancreatic CancerCancer cellAugust 10, 2020Escobar-Hoyos LF, Penson A, Kannan R, Cho H, Pan CH, Singh RK, Apken LH, Hobbs GA, Luo R, Lecomte N, et al.active RAS (Cell signaling, 8821), active Rho (Cell signaling, 8820), active Rap1 (Cell signaling, 8818) following the manufacturer’s instructions. Levels of active RAS small-GTPases were determined by western blotting

(10) Rap1-GTPases control mTORC1 activity by coordinating lysosome organization with amino acid availabilityNature communicationsMarch 17, 2020Mutvei AP, Nagiec MJ, Hamann JC, Kim SG, Vincent CT, Blenis J.the Rap1 binding domain (RBD) of Ral GDS from EMD-Millipore (14–455) or Cell Signaling Technologies (8818), according to the manufacturers protocol. The assay was validated per the manufacturer’s instructions. Lysosome

(11) Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriersThe Journal of cell biologyAugust 6, 2018Hilfenhaus G, Nguyen DP, Freshman J, Prajapati D, Ma F, Song D, Ziyad S, Cuadrado M, Pellegrini M, Bustelo XR, et al.Abcam). For detection of activated Rap1, Rac1, and Rho, a GTP-bound GTPase pulldown assay was used (8818, 8815, and 8820; Cell Signaling Technology) and conducted according to the manufacturer’s instructions.

(12) A small molecule inhibitor of Rheb selectively targets mTORC1 signalingNature communicationsFebruary 7, 2018Mahoney SJ, Narayan S, Molz L, Berstler LA, Kang SA, Vlasuk GP, Saiah E.serum-starved in DMEM (no FBS) overnight and lysed following the manufacturer’s instructions (CST #8818). Lysate was loaded with nucleotide (1 mM GDP or 0.1 mM GTPγS) and then incubated with NR1 for 30 min

(13) Rasa3 controls turnover of endothelial cell adhesion and vascular lumen integrity by a Rap1-dependent mechanismPLoS GeneticsJanuary 30, 2018Patricia Molina-Ortiz, Tanguy Orban, Maud Martin, Audrey Habets, Franck Dequiedt, Stéphane SchurmansDetection of active GTP-bound Ras and Rap1 in HUVECs was performed with Cell Signaling kits (#8821 and #8818, respectively). The low molecular Rap1 inhibitor GGTI298 was from Sigma. Control (SR-CL000-005) and

(14) RAPGEF5 Regulates Nuclear Translocation of β-CateninDevelopmental cellJanuary 22, 2018Griffin JN, Del Viso F, Duncan AR, Robson A, Hwang W, Kulkarni S, Liu KJ, Khokha MK.interacting proteins, were then eluted. The active rap detection kit (Cell signaling Technologies #8818) was used as per the manufacturer’s instructions. Samples were analyzed by Western blot. Nuclear/cytoplasmic

(15) p53-mediated miR-18 repression activates HSF2 for IGF-IIR-dependent myocyte hypertrophy in hypertension-induced heart failureCell death & diseaseAugust 10, 2017Huang CY, Pai PY, Kuo CH, Ho TJ, Lin JY, Lin DY, Tsai FJ, Padma VV, Kuo WW, Huang CY.

(16) EGCG, a green tea catechin, attenuates the progression of heart failure induced by the heart/muscle-specific deletion of MnSOD in miceJournal of cardiologyFebruary 1, 2017Oyama JI, Shiraki A, Nishikido T, Maeda T, Komoda H, Shimizu T, Makino N, Node K.

(17) Loss of phosphodiesterase 4D mediates acquired triapine resistance via Epac-Rap1-Integrin signalingOncotargetDecember 20, 2016Walter Miklos, Petra Heffeter, Christine Pirker, Sonja Hager, Christian R. Kowol, Sushilla van Schoonhoven, Mirjana Stojanovic, Bernhard K. Keppler, Walter Bergertreated according to manufacturer's protocol (Cell Signaling Technology; Active Rap1 Detection Kit, #8818). Xenograft experiments and immunohistochemistry Six to eight week old male CB-17 scid/scid (SCID)

(18) Intracellular ascorbate tightens the endothelial permeability barrier through Epac1 and the tubulin cytoskeletonAmerican journal of physiology. Cell physiologyOctober 1, 2016Parker WH, Rhea EM, Qu ZC, Hecker MR, May JM.MD). Immunoprecipitation. Active Rap1 was immunoprecipitated using a kit from Cell Signaling Technology (catalog no. 8818; Danvers, MA) following the manufacturer’s instructions. ␣-Tubulin-bound Epac1 was immunoprecipitated

(19) The microscale flocculation test (MFT)â€”A high-throughput technique for optimizing separation performanceChemical Engineering Research and DesignJanuary 1, 2016Ryan J. LaRue, Jeffrey Cobbledick, Nicholas Aubry, Emily D. Cranston, David R. Latulippehigh molecular weight, medium charge density (8814); very high molecular weight, high charge density (8818); high molecular weight, medium charge density (8844FS); high molecular weight, very-high charge density

(20) Anterior gradient protein 2 expression in high grade head and neck squamous cell carcinoma correlated with cancer stem cell and epithelial mesenchymal transitionOncotargetApril 20, 2015Ma SR, Wang WM, Huang CF, Zhang WF, Sun ZJ.Survivin (Cell Signaling Technology, 1:200), Cyclin D1 (Epitomics, www.impactjournals.com/oncotarget 8818 Oncotarget ACKNOWLEDGMENTS 61(2):69-90. 10. Warnakulasuriya S. Global epidemiology of oral and oropharyngeal cancer

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Active Rap1 Detection Kit

Active Rap1 Detection Kit from Cell Signaling Technology

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Cell Signaling Technology

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Active Rap1 Detection Kit from Cell Signaling Technology

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