SignalStain ® DAB Substrate Kit 8059S from Cell Signaling Technology

Product Specs
ItemSignalStain ® DAB Substrate Kit
CompanyCell Signaling Technology
Price $194.00 Inquire
Catalog Number8059S
Quantity1 Kit
Research AreaRelated Products
ApplicationsIHC-P

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) The CHI3L1-neutrophil axis drives immune suppression and breast cancer metastatic disseminationJCI InsightMarch 23, 2026Tarek Taifour, Adéline Massé, Yu Gu, Virginie Sanguin-Gendreau, Dongmei Zuo, Bin Xiao, Emilie Solymoss, Yunyun Shen, Hailey Proud, Sherif Samer Attalla, et al.procedure was repeated, but the following substrate was used: 3,30-diaminobenzidine (DAB) (Cell Signaling 8059). In this case, slides were counterstained with hematoxylin for 30 seconds. Sections were scanned using

(2) Inhibition of Autophagy Reveals ATR Protein Kinase as a Key Mediator of Cisplatin Sensitivity in OsteosarcomaCancer MedMarch 5, 2026Janice S. Pereira, Gabriel Rosa, Aine Pears, Beata Burczynska, Britta Stordal, Scott J. Roberts, Helen C. Robertsapplied for 30 min at RT and stained with SignalStain DAB Substrate Kit (Cell Signaling Technology, 8059P). The nuclear counterstain was carried out using hematoxylin. Images were obtained using Eclipse 50i

(3) Differential effects on acetaminophen-induced nephrotoxicity and liver injury following modulation of glutathione resynthesisFood and Chemical ToxicologyFebruary 1, 2026Yasaman Etemadi, Jephte Y. Akakpo, Timothy A. Fields, Anup Ramachandran, Hartmut Jaeschke(Vector Laboratories, PK-4001) followed by a diamond-benzidine (DAB) substrate kit (Cell Signaling Technology, #8059). 2.5. RNA isolation and quantitative PCR Total RNA was isolated from liver and kidney tissues using

(4) Inhibition of focal adhesion kinase impairs tumor formation and preserves hearing in a murine model of NF2-related schwannomatosisScience AdvancesJanuary 30, 2026Dana K. Mitchell, Kylee Brewster, Li Jiang, Henry Mang, Waylan K. Bessler, Xiaohong Li, Qingbo Lu, Shaomin Qian, Eric York, Sarah K. Morrow, et al.temperature [goat anti-rabbit, ab205718, Abcam (1:1000)], followed by 3,3′-Diaminobenzidine (DAB) (#8059, Cell Signaling Technology), which was applied for 10 min and terminated by rinsing in distilled water.

(5) PDX1 phosphorylation at S61 by mTORC1 links nutrient signaling to β cell function and metabolic diseaseCell ReportsJanuary 27, 2026Jialin Fan, Xin Zhang, Jinling Zhang, Tinghan Zhao, Stephen K. Burley, X.F. Steven ZhengFor IHC, detection was performed with the Elite ABC Kit (PK-6100, Vector Laboratories) and DAB Substrate (8059, Cell Signaling Technology). Epredia Cytoseal Mounting medium (831016, Thermo Fisher Scientific) was

(6) Impaired nitrogenous waste clearance promotes hepatocellular carcinomaScience AdvancesJanuary 9, 2026Xinlu Han, Jianliang Shen, Junrong Yan, Rahul Tacke, Weiwei Dai, Qingqing Mao, Heineken Queen Daguplo, Shuyang Liu, Ariful Islam, Tong Liu, et al.applied for 30 min. Peroxidase substrate solution 3,3′-diaminobenzidine (Cell Signaling Technology, #8059S) was applied for proper time until desired color reaction is observed. The reaction was terminated by

(7) Transient receptor potential canonical 3 is required for HPV-induced malignant transformation of cervical epithelial cellsCommunications BiologyJanuary 8, 2026Yudi Tan, Qiaoqiao Huang, Jingjing Zeng, Yong Wang, Shasha Yang, Li Yu, Jinkong Wei, Yuying Wei, Jinmin Zhao, Junying Chenspecific primary antibodies as mentioned above and DAB substrate kit (Cell Signaling Technology, Cat#8059). TUNEL staining was performed on mouse cervical sections according to the instruction of manufacturer

(8) Intrathecal CRISPR-edited allogeneic IL-13Rα2 CAR T Cells for recurrent high-grade Glioma: preclinical characterization and phase I trialNature CommunicationsJanuary 6, 2026Xuetao Li, Xiaoyun Shang, Jiangang Liu, Yang Zhang, Xian Jia, Huabing Li, Yihan Wang, Jianen Gao, Xu Ma, Xuewen Zhang, et al.secondary antibody (Cat. #7074, CST). Visualization was achieved using 3,3′-diaminobenzidine (DAB; Cat.#8059, CST) as the chromogenic substrate, and sections were counterstained with hematoxylin. IL-13Rα2 antigen–positive

(9) Palmitoylation of Tfr1 enhances platelet ferroptosis and liver injury in heat strokeActa Pharmaceutica Sinica BJanuary 1, 2026Qiyuan An, Riqing Wei, Zhicheng Huang, Youyong Tang, Minghao Wang, Sixiao He, Kaihua Huang, Zhifeng Liu, Meimei Zhang, Ru Li, et al.30 min at room temperature. Following this, the slides were incubated with DAB substrate solution (8059, Cell Signaling Technology, Danvers, MA, USA) until the color developed. Images were captured and visualized

(10) Targeting mTORC1 to promote ferroptosis and apoptosis in endometrial cancer with PI3K-Akt-mTOR pathway mutationFerroptosis and Oxidative StressDecember 29, 2025Yingying Hu, Pei Liu, Neal Rosen, Xuejun Jiang30 min. Slides were then stained with the SignalStain® DAB Substrate Kit (Cell Signaling Technology 8059S) followed by Hematoxylin (Cell Signaling Technology 14166S), and imaged under a microscope. The following

(11) Tumor-associated high endothelial venules are associated with enhanced lymphocyte infiltration and favorable prognosis in resected hepatocellular carcinomaCancer Immunol ImmunotherDecember 19, 2025Shu Aoyama, Takehiro Noda, Hirofumi Akita, Yosuke Mukai, Kazuki Sasaki, Shinichiro Hasegawa, Daisaku Yamada, Yoshito Tomimaru, Tadafumi Asaoka, Hidenori Takahashi, et al.peroxidase labeling reagent (Histofine series, Nichirei Bioscience), and staining was conducted with DAB (#8059, Cell Signaling). Supplemental Table S1 lists the antibodies used for immunohistochemical staining. Next,

(12) Utilizing nanoinducers for precision degradation of bacterial protein to mitigate antibiotic resistanceNat CommunDecember 18, 2025Linqi Han, Wenping Huang, Xueting Pan, Ziying Fu, Hao Peng, Guangjun Nie, Hai Wang1 h at room temperature. Afterwards, each section was incubated with 100 μl SignalStain® DAB (CST #8059) for 5 min and immersed in deionized water three times. The slides were examined using an inverted microscope. In

(13) Multi-omics factor analysis identifies the Tensin 1-Fermitin family homologue 2-Fibronectin 1-Integrin signaling axis as a prognostic determinant in colorectal cancerMol BiomedDecember 17, 2025Tianwei Chen, Yebin Yang, Jing Shi, Fanhe Dong, Lesi Xie, Yuqiang Shan, Xiang Wangtimes followed by incubation with SignalStain® Boost IHC detection Reagent (Cell Signaling Technology, 8059) for 30 min in a moist atmosphere in darkroom. Tyramide signal amplification staining (1:100) was used

(14) The endothelial growth factor angiopoietin-2 is an accurate prognostic biomarker in patients with acetaminophen-induced acute liver failureToxicological SciencesDecember 1, 2025David S Umbaugh, Nga T Nguyen, Steven C Curry, Jody A Rule, William M Lee, Anup Ramachandran, Hartmut Jaeschke, Acute Liver Failure Study Group, Shannan Tujios, David Koch, et al.followed by visualization using SignalStain Dab Chromogen concentrate (Cell Signaling Technologies, No. 8059) with images captured using an Olympus BX51TF microscope. ELISA assay Circulating ANGPT2 was measured

(15) Molecular insights into treatment-resistant erectile dysfunction: staining patterns of human corpora cavernosa—a preliminary studyTranslational Andrology and UrologyNovember 30, 2025Kunj Jain, Marvin A. Simpkins, Meher Pandher, Aleksander Popovic, Evan Kovac, Lawrence Wyner, Robert E. Weiss, Amjad AlwaalPolymer kit (Vector, Newark, CA, USA; MP-7451-50) with DAB chromogen (Cell Signaling, Danvers, MA, USA; 8059S). Antigen retrieval was performed in citrate buffer, pH 6.0 (Novus, Centennial, CO, USA; NB900-62075).

(16) The nucleobase guanine at the 3’-terminus of oligonucleotide RGLS4326 drives off-target AMPAR inhibition and CNS toxicityNature CommunicationsNovember 28, 2025Tania Valencia, Laura Y. Yen, Cindy Berman, Thomas Vincent, Scott Davis, Francesca Varrone, Jianfeng Huang, Jessica Mastroianni, Morgan Carlson, Tate Owen, et al.30 min at room temperature (RT). DAB substrate kit (Signal Stain DAB Substrate Kit, Cell Signaling, 8059) was used for detection and samples were incubated for 5 min at RT. Hematoxylin was used for nuclear

(17) Glucose starvation mimetic aldometanib removes immune barriers permitting mice with hepatocellular carcinoma to live to normal agesCell ResearchNovember 25, 2025Hui-Hui Hu, Xuefeng Wang, Bin Lan, Haili Cheng, Hong Wen, Fangfang Chen, Jianfeng Wu, Mengqi Li, Jiazhou Chen, Jinhui Zhang, et al.with gDNA Remover (Cat# FSQ-301) was purchased from Toyobo. The SignalStain DAB Substrate Kit (Cat# 8059) was purchased from Cell Signaling Technology. LabAssay triglyceride reagent (Cat# 290-63701) was purchased

(18) Integrated proteomic analysis identifies TYMS-dependent AMPK-mTOR signaling in pancreatic neuroendocrine tumorsiScienceNovember 21, 2025Vinod Vijayakurup, Benjamin Meyer, Syeda Rida Rizvi, Kara H. Kievit, Iram Hyder, Jin Koh, Michael Feely, Maria V. Guijarro, Frederic J. Kaye, Maria Zajac-Kayewas discarded, and slides washed in TBS three times before application of Cell Signaling DAB (CST #8059) substrate diluted according to kit instructions. DAB reaction was allowed to progress for up to 10-min

(19) Modeling antithymocyte globulin-induced microvasculopathy using human iPSC-derived vascularized liver organoidsCell Reports MedicineNovember 6, 2025Shuntaro Kawamura, Yosuke Yoneyama, Norikazu Saiki, Yunheng Wu, Chiharu Moriya, Rio Ohmura, Mari Maezawa, Yoshihiro Shimada, Yicheng Wang, Kensaku Mori, et al.8125S). Color development was achieved using SignalStain DAB Substrate Kit (Cell Signaling Technology; 8059), followed by hematoxylin counterstaining. Sections were imaged using a BZ-X fluorescence microscope

(20) NRF2 activation in cancer cells suppresses immune infiltration into the tumor microenvironmentiScienceOctober 17, 2025Huaichun Wen, Takafumi Suzuki, Anqi Zhang, Miu Sato, Mahiro Matsumoto, Yuka Takahashi, Yushi Takahashi, Masayuki YamamotoSet,MGI,Cat#1000016995 Protein Block Serum-Free,Dako,Cat#X0909 SignalStain DAB Substrate Kit,Cell Signaling Technology,Cat#8059S Puromycin dihydrochloride,Invitrogen,Cat#A1113803 ,, Deposited data,Deposited data,Deposited data ,, RNA-seq

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SignalStain ® DAB Substrate Kit

SignalStain ® DAB Substrate Kit from Cell Signaling Technology

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SignalStain ® DAB Substrate Kit from Cell Signaling Technology

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