Description
Mouse IFN-α ELISA kit has been developed to quantitate levels of IFN-α in cell/tissue culture media (10% FBS) using a sandwich immunoassay. The quantitation of mouse interferon alpha in serum and plasma samples using this product has not been fully evaluated by PBL. The kit is based on an ELISA with anti-detection antibody conjugated to horseradish per-oxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The kit contains all reagents required to perform either 1 x or 5 x 96-well plates. The assay is based on the international reference standard for mouse interferon alpha (Mu-IFN-α) provided by the National Institutes of Health. Typical standard curves for each lot are included with the procedure. It is important with this kit and any ELISA kit to run the standard curve in the same matrix as your samples unless you have demonstrated that the matrix does not affect the signal