Human Midkine DuoSet ELISA DY258 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman Midkine DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY258
Quantity1 Kit (for 15 Plates)
ApplicationsELISA
Detection Range78.10 - 5000 pg/mL
ReactivityHuman
Detection TargetMidkine
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Namemidkine
NCBI Gene AliasesARAP, MK, NEGF2

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Serum Midkine as a Potential Biomarker Associated with Disease Severity and Remodeling-Related Mediators in Children with AsthmaJ Asthma AllergyJanuary 20, 2026Yongtian Xu, Lixia Zhao, Yingying Mi, Yi Ge, Yanfang GuoEnzyme-linked Biotechnology; LoD: 18 pg/mL), hyaluronan (DHYAL0, R&D Systems; LoD: 0.2 ng/mL), and midkine (DY258, R&D Systems; LoD: 78.1 pg/mL) in both children with asthma and healthy control subjects, according

(2) Serum Midkine as a Potential Biomarker Associated with Disease Severity and Remodeling-Related Mediators in Children with AsthmaJournal of Asthma and AllergyJanuary 20, 2026Yongtian Xu, Lixia Zhao, Yingying Mi, Yi Ge, Yanfang GuoEnzyme-linked Biotechnology; LoD: 18 pg/mL), hyaluronan (DHYAL0, R&D Systems; LoD: 0.2 ng/mL), and midkine (DY258, R&D Systems; LoD: 78.1 pg/mL) in both children with asthma and healthy control subjects, according

(3) Midkine promotes PDGF-BB-induced proliferation, migration, and glycolysis of airway smooth muscle cells via the PI3K Akt pathwayPhysiological reportsSeptember 1, 2025Xu Y, Li W, Shi J, Guo Y.20 individuals diagnosed with asthma and 20 healthy individuals. The concentrations of midkine (Cat#DY258, R&D Systems, Minneapolis, USA) in the serum were quantified. In addition, culture media from ASMCs

(4) Blocking the functional domain of cancer cell surface TIP1 upregulates Midkine via the β-catenin/Wnt signaling pathwayCancer Gene TherapyJuly 1, 2025Minakshi Saikia, Harendra Kumar Shah, Dennis E. Hallahan, Abhay Kumar Singh, Vaishali Kapoordead. ELISA Midkine secreted into the media was detected using the Human Midkine DuoSet ELISA kit (R&D system #DY258) following the manufacturer’s protocol. Nunc Maxisorp Flat bottom plates for ELSA (Invitrogen #44–2404-21)

(5) Ugonin P mitigates osteolytic bone metastasis by suppressing MDK via upregulating miR-223-3p expressionInt J Biol SciMay 31, 2025Yat-Yin Law, Haritha Rengamanar, Chih-Ying Wu, Chih-Chuang Liaw, Shubham Suresh Ghule, Yu-Ying Wu, Kuan-Ying Lai, Le Huynh Hoai Thuong, Trung-Loc Ho, Athena Yanjen Lin, et al.collected for further analysis 35. MDK level in the CC-CM was quantified using an ELISA kit (Calalog no: DY258, R&D Systems, MN, USA) following the manufacturer's protocol. Briefly, samples were incubated in 96-well

(6) Development of bead based multiplexed immunoassay for evaluation of midkine, syndecan-1, and ANGPTL4 in patient serumJournal of immunoassay & immunochemistryJanuary 1, 2018Tarhoni I, Fhied CL, Pool M, Liptay MJ, Bonomi P, Seder CW, Borgia JA.Human Angiopoietin-like 4 DuoSet ELISA® (DY3485), Human Syndecan-1 DuoSet ELISA® (DY2780), and Human Midkine DuoSet ELISA® (DY258) assays by R&D systems), as well as include parameters from similar assays in the ELISA format for comparison.

(7) Midkine promotes hepatocellular carcinoma metastasis by elevating anoikis resistance of circulating tumor cellsOncotargetMay 16, 2017Sun B, Hu C, Yang Z, Zhang X, Zhao L, Xiong J, Ma J, Chen L, Qian H, Luo X, et al.

(8) Serum midkine as a surrogate biomarker for metastatic prediction in differentiated thyroid cancer patients with positive thyroglobulin antibodyScientific reportsFebruary 27, 2017Jia Q, Meng Z, Xu K, He X, Tan J, Zhang G, Li X, Liu N, Hu T, Zhou P, et al.

(9) Validation of the diagnostic utility of urinary midkine for the detection of bladder cancerOncology lettersNovember 1, 2016Vu Van D, Heberling U, Wirth MP, Fuessel S.midkine was determined in urine supernatants using the commercially available Human Midkine DuoSet ELISA (DY258; R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer's instructions, except for the

(10) Midkine, a potential link between obesity and insulin resistancePloS oneJanuary 1, 2014Fan N, Sun H, Wang Y, Zhang L, Xia Z, Peng L, Hou Y, Shen W, Liu R, Peng Y.

(11) Combined proteome and transcriptome analyses for the discovery of urinary biomarkers for urothelial carcinomaBritish Journal of CancerMay 14, 2013N J Shimwell, R T Bryan, W Wei, N D James, K K Cheng, M P Zeegers, P J Johnson, A Martin, D G Wardvalidation Candidate biomarkers were measured in urine and serum sample using commercially available ELISA kits (DY258, DY1048 and DY1298, R&D systems, Abingdon, UK) according to the manufacturer's instructions. Standards

(12) ERβ-mediated estradiol enhances epithelial mesenchymal transition of lung adenocarcinoma through increasing transcription of midkineMolecular endocrinology (Baltimore, Md.)August 1, 2012Zhao G, Nie Y, Lv M, He L, Wang T, Hou Y.

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Midkine ELISA

Human Midkine DuoSet ELISA

Human Midkine DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Human Midkine DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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