12(S)-HETE ELISA Kit ab133034 from Abcam

Product Specs
Item12(S)-HETE ELISA Kit
CompanyAbcam
Price $519.00Supplier Page View Company Product Page
Catalog Numberab133034
Quantity1 x 96 Tests
ApplicationscELISA, ELISA
Research AreaMetabolism
SensitivityEqual to 146.3 pg/mL
Detection Range195 - 50000 pg/ml

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Elevated maternal pre-transfer serum lipid peroxidation is associated with implantation failure and early pregnancy lossBMC MedicineJanuary 12, 2026Zhangwei Jia, Junxiang Ren, Tianxiang Ni, Xinyu Liu, Yuhan Shao, Wei Zhou, Qian Zhang, Chenxi Jin, Jia Yuan, Junhao Yanabsorbance measured at 535 nm. 12-HETE and 15-HETE were quantified using commercial ELISA kits (Abcam, ab133034 and ab133035), and T-AOC and SOD activity were assessed using kits from Nanjing Jiancheng (A015-2–1,

(2) Loss of Bile Salt Export Pump (Bsep/Abcb11) Ameliorates Toxin-induced Hepatic Fibrosis via Suppression of Hepatocellular Jun Amino-terminal Kinase Signaling and Hepatic Stellate Cell ActivationCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2026Claudia D. Fuchs, Emmanuel D. Dixon, Philipp Königshofer, Thierry Claudel, Veronika Mlitz, Hubert Scharnagl, Tatjana Stojakovic, Thomas Reiberger, Michael Traunerlevels were measured in serum of animals according to the manufacturers’ manuals (Abcam; product number: ab133034). Statistical Analysis Results were evaluated using GraphPad Prism 8.4.1. Statistical analysis was

(3) Whole-gene CRISPR/cas9 library screen revealed targeting STAT6 increased the sensitivity of liver cancer to celecoxib via inhibiting arachidonic acid shuntingCell Commun SignalAugust 28, 2025Chujiao Hu, Zhirui Zeng, Xin Bao, Dahuan Li, Huading Tai, Haohao Zeng, Cheng Luo, Lei Tang, Tengxiang Chen, Shi Zuothe Prostaglandin E2 ELISA Kit (cat. no. ab287802, Abcam, USA), the 12(S)-HETE ELISA Kit (cat. no. ab133034, Abcam, USA), and the 14,15-EET ELISA Kit (cat. no. ab175812, Abcam, USA), respectively. The relative

(4) The deubiquitinase OTUD3 plays a neuroprotective role by reducing ferroptosis induced by cerebral ischaemia reperfusion via stabilizing PLK1 via deubiquitinationClin Transl MedJune 3, 2025Jing Cheng, Qi Tian, Hao‐Ran Lu, Hong‐Xiang Jiang, Xiao‐Hong Qin, Yan‐Qin Fan, Zhi‐Biao Chen, Li‐Quan Wuand reliable quantification of 5‐HETE levels in the samples. 4 12‐HETE ELISA The 12‐HETE ELISA kit (ab133034; Abcam) was used to quantify 12‐HETE levels in the samples, following the detailed experimental protocol

(5) Elevated NGF provokes decidual lipid peroxidation and promotes preterm birth in miceJ Transl MedApril 28, 2025Yuhang Ge, Ruxin Teng, Zhaoyu Jia, Yongyue Li, Yafang Lu, Jia Yuanmeasurement of 12(S)-HETE was conducted in accordance with the protocol provided in the reagent kit (Abcam, ab133034). Transmission electron microscopy Fresh mouse decidual tissue was fixed in 2.5% glutaraldehyde/0.1

(6) 12/15-Lipooxygenase Inhibition Reduces Microvessel Constriction and Microthrombi After Subarachnoid Hemorrhage in MiceTranslational Stroke ResearchSeptember 19, 2024Ari Dienel, Sung Ha Hong, Hussein A. Zeineddine, Sithara Thomas, Shafeeque C. M., Dania A. Jose, Kiara Torres, Jose Guzman, Andrew Dunn, P. Kumar T., et al.was stored at ͨ − 80 °C until measurement. 12-S-HETE concentration was measured using an ELISA kit (ab133034, Abcam) according to manufacturer guidelines. Immunostaining One Sham and one SAH male C57BL/6 mouse

(7) Meningioma achieves malignancy and erastin-induced ferroptosis resistance through FOXM1-AURKA-NRF2 axisRedox BiologyJune 1, 2024Yangfan Ye, Lei Xu, Liuchao Zhang, Pengzhan Zhao, Wanzhi Cai, Guoqiang Fu, Tian Wang, Zeqiang Tao, Wenqian Shi, Wei Gu, et al.12-HETE and 15-HETE levels detection 12/15-HETE levels were assessed using a 12/15-HETE ELISA kits (ab133034/ab133035, Abcam) according to the manufacturer's instructions and our previous study [6]. 2.26 Xenograft

(8) O-sialoglycoprotein Endopeptidase (OSGEP) Suppresses Hepatic Ischemia-Reperfusion Injury-Induced Ferroptosis Through Modulating the MEK/ERK Signaling PathwayMolecular BiotechnologyMarch 8, 2024Yuanyuan Tao, Wanqing Zhou, Cheng Chen, Qian Zhang, Zhuoyi Liu, Pingping Xia, Zhi Ye, Chunling Liindicates fluorescence intensity of detected sample). 12/15-HETE Assay Through 12/15-HETE ELISA kits (#ab133034/#ab133035; Abcam), it was attempted to measure 12/15-HETE levels on the basis of instructions provided

(9) FLRT2 suppresses bladder cancer progression through inducing ferroptosisJournal of Cellular and Molecular MedicineMarch 1, 2024Pengcheng Jiang, Jinzhuo Ning, Weimin Yu, Ting Rao, Yuan Ruan, Fan Cheng(#ab102526), Lipid Peroxidation Assay Kit (#ab118970), 12(S)‐hydroxyeicosatetraenoic acid (HETE) ELISA Kit (#ab133034), 15(S)‐HETE ELISA Kit (#ab133035), Iron Assay Kit (#ab83366) and Glutathione (GSH) Assay Kit (#ab239727)

(10) Inhibition of ferroptosis by POLE2 in gastric cancer cells involves the activation of NRF2/GPX4 pathwayJournal of Cellular and Molecular MedicineJanuary 1, 2024Hui Jian, Zhi‐Qiang Chen, Heng Du, Ting Liao, Yi‐Chen Sun, Dong Ke, Yang Yureader. 2.9 12/15‐HETE assay 12/15‐HETE levels in cell medium were evaluated using 12/15‐HETE ELISA kits (ab133034/ab133035; Abcam) following the manufacturer's instructions. 2.10 Measurements of GSH level and GPX4

(11) Hematopoietic 12/15-lipoxygenase activity negatively contributes to fungal-associated allergic asthmaAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologyAugust 1, 2023Mgayya Makullah, Diandra A. Ellis, MaryJane Jones, Chad Steele12-HETE levels were quantified using 12(S)-HETE ELISA kit per the manufacturer’s instructions (Cat. AB133034, Abcam, Waltham, MA). Whole Lung Cytokine and Chemokine Analysis Following euthanization, the right

(12) Hsp90 induces Acsl4-dependent glioma ferroptosis via dephosphorylating Ser637 at Drp1Cell Death & DiseaseJune 13, 2022Zong Miao et al.from Selleck Chemicals. 12-HETE and 15-HETE levels were detected respectively by 12-HETE ELISA kits (ab133034, Abcam) and 15-HETE ELISA kits (ab133035, Abcam) according to the manufacturer’s instructions. GSH

(13) Ferroptosis is involved in the anti-tumor effect of lycorine in renal cell carcinoma cellsOncology lettersNovember 1, 2021Du Y, Zhao HC, Zhu HC, Jin Y, Wang L.ferroptotic markers (25). 12- or 15-HETE levels were determined using 12- or 15-HETE ELISA kits (cat. nos. ab133034 and ab133035; Abcam), according to the manufacturer's protocols. Each 96-well plate includes control,

(14) Prokineticin-2 prevents neuronal cell deaths in a model of traumatic brain injuryNature communicationsJuly 9, 2021Bao Z, Liu Y, Chen B, Miao Z, Tu Y, Li C, Chao H, Ye Y, Xu X, Sun G, et al.Beyotime, China). 12-HETE and 15-HETE levels 12/15-HETE levels were assessed using 12/15-HETE ELISA kits (ab133034/ab133035, Abcam) according to the manufacturer’s instructions. Protein silver staining and qualitative

(15) Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosisOncology lettersOctober 1, 2020Tan S, Hou X, Mei L.15‑HETE, which are two ferroptotic markers, were determined using 12/15‑HETE ELISA kits (cat. nos. ab133034 and ab133035; Abcam), according to the manufacturer's protocols. Lactate dehydrogenase (LDH) assay.

(16) ACSL4 suppresses glioma cells proliferation via activating ferroptosisOncology reportsJanuary 1, 2020Cheng J, Fan YQ, Liu BH, Zhou H, Wang JM, Chen QX.protocol. 12/15-HETE assay 12/15-HETE levels were assessed using 12/15-HETE ELISA kits (cat. nos. ab133034 and ab133035; Abcam), according to the manufacturer's protocols. Each 96-well plate, contained control,

(17) Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusionCell death and differentiationNovember 1, 2019Li Y, Feng D, Wang Z, Zhao Y, Sun R, Tian D, Liu D, Zhang F, Ning S, Yao J, et al.manufacturer’s instructions. 12/15-HETE assay 12/15-HETE levels were assessed using 12/15-HETE ELISA kits (ab133034/ab133035, Abcam) according to the manufacturer’s instructions. Real-time PCR Total RNA was isolated

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12(S)-HETE ELISA Kit from Abcam

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12(S)-HETE ELISA Kit from Abcam

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