Fig 1: Gata5-null mice have low-renin hypertension.(a) Increased systolic and diastolic BP in 90-day-old Gata5-null mice. Both genders are hypertensive (males n=14–16 per group; females n=11–12 per group). The results are reported as mean±s.e.m. *P<0.05; ***P<0.005 (two-factor ANOVA). (b) Hypertension in Gata5-null mice is sensitive to diuretic: hydrochlorothiazide (HCTZ) for 5 days lowered, but did not normalize BP in Gata5-null mice at both low (+, 2.8 mg per day) and high dose (++, 8 mg per day) (n=4 per group). The results are reported as mean±s.e.m. *P<0.05 versus Gata5+/+ mice; ***P<0.01 versus Gata5+/+ mice; #P<0.05 versus untreated mice, ##P<0.01 versus untreated mice (repeated measures two-factor ANOVA test followed by Bonferonni correction for multiple comparisons). (c) Low-renin hypertension in Gata5-null mice: the expression of Ace (angiotensin-converting enzyme) gene in the lung was unchanged, while expression of Ren (renin) in the kidney and Agt (angiotensinogen) gene in the liver was decreased. The expression of these genes was measured by qPCR (n=3–4 per group). The results are reported as mean±s.e.m. *P<0.05 versus Gata5+/+ mice (Mann–Whitney test). (d) Plasma renin activity (PRA) was decreased in Gata5-null mice versus their control littermates (n=8–10 per group). The results are reported as mean±s.e.m. *P<0.05 versus Gata5+/+ mice (t-test). (e) There are no changes in aldosterone urinary concentration as measured by the enzyme immunoassay. (n=6 per group). The results are reported as mean±s.e.m. (t-test).