Fig 1: A Timeline of gene expression of SMPD1-3 in HCAECs after hyperglycemic injury, presented as 2-ddCT vs GAPDH, n = 6. B Acidic and neutral SMase enzyme activity in HCAECs after 72 h of hyperglycemic injury, n = 5–6. C + D Immunoblotting for nSMase2 and ß-Actin after 72 h of hyperglycemic injury, with quantification, n = 3. E MTT viability assay in HCAECs that were treated with lEVs from HCAECs after hyperglycemic injury, n = 6. F Flow-cytometric analysis of the induction of apoptosis in HCAECs that were treated with lEVs from HCAECs after hyperglycemic injury, (right) representative dot blots, n = 5. G MTT viability assay in HCAECs after treatment with mannitol, n = 6. H Flow-cytometric analysis of the induction of apoptosis in HCAECs that were treated with lEVs from HCAECs after osmotic injury with mannitol, n = 3. All data are presented as individual experiments with the mean ± SEM; n.s. not significant, *p < 0.05, ANOVA + Bonferoni’s multiple comparison test were used for A, E, G, and unpaired t-Test for B, D, F, H
Fig 2: The effect of pinealectomy on ASMase level (A) and activity (B) in the hippocampus in 3, 14-, and 18-month-old rats. Data are presented as mean ± SEM. Two-way ANOVA indicated a main age effect [F2,35 = 6.584, p = 0.003], surgery effect [F1,46 = 7.223, p = 0.010] for ASMase level; ** p = 0.002, 14-month-old sham rats vs. 3-month-old sham rats; and ** p = 0.005, 18-month-old sham rats vs. 3-month-old sham rats (A). Two-way ANOVA showed a major age effect for the ASMase activity: F2,45 = 647.244, p < 0.001], and *** p < 0.001, 18-month-old sham rats vs. 3- and 14-month-old sham rats (B).
Supplier Page from Abcam for Sphingomyelinase Assay Kit (Colorimetric)