Fig 1: The different CTs provoke diagnostic transcriptional responses and the uptake of fatty acids is unaffected by CT2.We investigated the transcriptional response of S3 cells after 4 h of CT treatment in the presence or absence of 200 μM OA with either 1 μM active (CT1 = THI-4, CT2 = TPE-69, or CT3 = AU-6) or 1 μM inactive (CT1 = THI-68, CT2 = TPE-67, or CT3 = AU-73) compound derivatives. (a) Expression profiles showing differentially expressed (DE) genes between treated and control samples. The scaled DE gene expression values (FPKM), Z scores, are presented in 5 k-means clusters of genes (rows). (b) Drosophila melanogaster specific KEGG metabolic pathways with highlighted reactions involved in small molecule responses (see color legend). (c) COS7 cells were kept in medium containing either DMSO, or 5 μM TPE-5 in DMSO. The cells were subsequently loaded with radiolabeled fatty acids, the cells were washed and internalized radioactivity was quantified by scintillation counting (bars provide mean ± standard deviation (s.d.), n = 3 wells). (d) Confocal images of Drosophila S3 cells incubated with the QBT-fluorescent fatty acid uptake reagent (Molecular Devices). On top of the fatty acid uptake reagent, the cell medium was either additionally supplemented with DMSO, DMSO and 800 μM OA or 5 μM TPE-5 solved in DMSO. Scale bar represents 10 μm. The following significance levels are used: not significant (n.s.) p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. Results between two independent groups were determined by Student's t-test. Also see Figures S3, S4 and Tables S1–S5 in the online version at http://dx.doi.org/10.1016/j.ebiom.2016.04.014.