Active Rho Detection Kit 8820S from Cell Signaling Technology

Product Specs
ItemActive Rho Detection Kit
CompanyCell Signaling Technology
Price $995.00 Inquire
Catalog Number8820S
Quantity1 Kit
Research AreaCytoskeletal Signaling
Molecular Weight21
Detection TargetRhoA
NCBI Full Gene Nameras homolog family member A
NCBI Gene AliasesARH12, ARHA, EDFAOB, RHO12, RHOH12

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Cell Signaling Technology

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Danvers, Massachusetts 01923

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Phone: 877-616-CELL
Phone: 978-867-2388
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Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) The Antidepressant Amitriptyline Upregulates ERK1/2 Signaling and Inhibits Rho-Mediated Responses Induced by Lysophosphatidic Acid in Astroglial CellsInt J Mol SciApril 20, 2026Maria C. Olianas, Simona Dedoni, Pierluigi Onaliexamined by using a commercial kit obtained from Cell Signaling Technology, Beverly, MA, USA (cat. no. 8820). Following exposure to the test agents, cells were washed with ice-cold PBS and lysed by the addition

(2) Integrin α8‐Mediated Pericyte Morphogenesis Controls Blood‐Brain Barrier IntegrityAdvanced ScienceOctober 14, 2025Chang‐Xiong Gong, Lin‐Lin Hu, Ling Jiang, Pei‐Xia Shi, Shuang Zhang, Yuan Zhao, Bing‐Qiao Wang, Xiao‐Feng Cheng, Cheng‐Kang He, Sen Lin, et al.RhoA Activation Assay GTP‐loaded RhoA levels were determined using a RhoA activation assay kit (CST, 8820) based on a pull‐down method. The assay involved binding GTP‐bound RhoA to glutathione resin via a GST‐linked

(3) Lipoprotein(a)/CD36 Interaction Drives IL-6/RhoA-GTP Signaling and miRNA Epigenetic Regulation in Coronary Artery SpasmPharmaceuticals (Basel)September 16, 2025Yen-Kuang Lin, Tsung-Han Hsieh, Chi-Tai Yeh, Vijesh Kumar Yadav, Iat-Hang Fong, Kuang-Tai Kuo, Nicholas G. Kounis, Patrick Hu, Ming-Yow HungGAPDH (Abcam, Cat #ab9484, Lot #GAP2203), RhoA (Cell Signaling Technology, Cat #2117), RhoA-GTP (Cat #8820), ROCK1 (Cat #4035), ROCK2 (Cat #9029), t-MBS (Cat #2634), and p-MBS (Cat #3040). Protein bands were

(4) Macrophages form dendrite-like pseudopods to enhance bacterial ingestionThe EMBO JournalJuly 28, 2025Changyuan Fan, Xinyi Huang, Jie Mei, Xuemeng Shi, Hao Zhang, Cong Liang, Shuzhi Cui, Yifan Xing, Biao Cao, Wei Liu, et al.the Rho-binding domain (RBD) of Rhotekin to GTP-RhoA with the commercial active Rho detection Kit (#8820, CST). Briefly, GTP-RhoA in cell lysates is affinity-purified using Rhotekin-RBD/GST fusion protein-coated

(5) Mmp14-dependent remodeling of the pericellular–dermal collagen interface governs fibroblast survivalJournal of Cell BiologySeptember 2, 2024Farideh Sabeh, Xiao-Yan Li, Adam W. Olson, Elliot Botvinick, Abhishek Kurup, Luis E. Gimenez, Jung-Sun Cho, Stephen J. Weiss(HRP-linked, anti-rabbit). Rho and Rho-GTP levels were determined using a Rho activation assay kit (#8820; Cell Signaling). Active TGF-β levels were determined in cell-free supernatants by ELISA (Legend Max Free Active TGF-β1 ELISA Kit

(6) Inflammatory lung injury is associated with endothelial cell mitochondrial fission and requires the nitration of RhoA and cytoskeletal remodelingFree Radical Biology and MedicineAugust 20, 2024Marissa D. Pokharel, Panfeng Fu, Alejandro Garcia-Flores, Manivannan Yegambaram, Qing Lu, Xutong Sun, Hoshang Unwalla, Saurabh Aggarwal, Jeffrey R. Fineman, Ting Wang, et al.mitochondrial networks. Measurement of RhoA activity The active Rho detection kit (Cell Signaling Technology #8820) was used to measure RhoA activity. After eNAMPT exposure, cells were lysed using RIPA lysis and extraction buffer

(7) Dual role of GRHL3 in bladder carcinogenesis depending on histological subtypesMolecular OncologyJune 1, 2024Franziska C. Lammert, Julia Pannhausen, Erik Noetzel, Florian Friedland, Julia Wirtz, Yannick Herfs, Sophie Leypold, Lin Gan, Ralf Weiskirchen, Tician Schnitzler, et al.225, Thermo Fisher Scientific). RHO kinase activation was measured by using Active RHO Detection Kit (#8820, Cell Signaling) according to the manufacturer's instructions. Provided spin cups were inserted into

(8) Alteration of actin cytoskeletal organisation in fetal akinesia deformation sequenceScientific reportsJanuary 19, 2024Jühlen R, Grauer L, Martinelli V, Rencurel C, Fahrenkrog B.used as input files (Supplementary Fig. S1, 11 online). Rho GTPase assay Active Rho Detection kit (#8820; Cell Signaling Technology/Bioke, Leiden, The Netherlands) was used to determine activation of Rho GTPase

(9) Interactions between insulin resistance, epigenetics, and donor sex in gene expression regulation of iPSC-derived myoblastsJournal of Clinical InvestigationJanuary 16, 2024Nida Haider, C. Ronald Kahnprotocol. The kit components, including the Rho rabbit antibody, was purchased from Cell Signaling (catalog 8820). For quantification of global DNA methylation, DNA was extracted from the differentiated iMyos, and

(10) Angiogenic and Fibrogenic Dual-effect of Gremlin1 on Proliferative Diabetic RetinopathyInternational journal of biological sciencesJanuary 1, 2024Wu X, Qin B, Cheng R, Zhou R, Wang X, Zhang Z, Mao X, Xie Z, Chen M, Jiang L, et al.RhoA was collected from 2 × 107 cells according to the manufacture's protocol (Cell Signaling, Cat#8820). Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred

(11) Actin Polymerization Defects Induce Mitochondrial Dysfunction in Cellular Models of Nemaline MyopathiesAntioxidants (Basel)November 21, 2023Rocío Piñero-Pérez, Alejandra López-Cabrera, Mónica Álvarez-Córdoba, Paula Cilleros-Holgado, Marta Talaverón-Rey, Alejandra Suárez-Carrillo, Manuel Munuera-Cabeza, David Gómez-Fernández, Diana Reche-López, Ana Romero-González, et al.BCA Protein Assay Kit (Fisher Scientific, Waltham, MA, USA) was used. A Rho Activation Assay Kit (Rf. 8820) (Cell Signaling Technology (Danvers, MA, USA)) was used. Rhodamine–phalloidin reagent (Abcam (Cambridge,

(12) scAAV2-Mediated Expression of Thioredoxin 2 and C3 Transferase Prevents Retinal Ganglion Cell Death and Lowers Intraocular Pressure in a Mouse Model of GlaucomaInternational Journal of Molecular SciencesNovember 13, 2023Hee Jong Kim, Seho Cha, Jun-Sub Choi, Joo Yong Lee, Ko Eun Kim, Jin Kwon Kim, Jin Kim, Seo Yun Moon, Steven Hyun Seung Lee, Keerang Park, et al.Active RhoA Assay The proportion of active RhoA was detected using an Active Rho Detection Kit (CST-8820S, Cell Signaling Technology). Briefly, HeLa cells were lysed with 1× Lysis/Binding/Wash buffer for 5

(13) ZXDC enhances cervical cancer metastasis through IGF2BP3-mediated activation of RhoA/ROCK signalingiScienceAugust 18, 2023Mao Y, Jiang X, Guo P, Ouyang Y, Chen X, Xia M, Wu L, Tang Z, Liang T, Li Y, et al.assays ,, Dual-Luciferase Reporter Assay Kit,Promega,Cat#E1910 Active Rho Detection Kit,Cell Signaling Technology,Cat#8820 SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads),Cell Signaling Technology,Cat#9002 ,, Deposited

(14) Uterus globulin associated protein 1 (UGRP1) binds podoplanin (PDPN) to promote a novel inflammation pathway during Streptococcus pneumoniae infectionClinical and translational medicineJune 1, 2022Han L, Zhang F, Liu Y, Yu J, Zhang Q, Ye X, Song H, Zheng C, Han B.Counting Kit (CCK‐8),YEASEN,Cat# 40203ES60 Active Rho Detection Kit,Cell Signaling Technology,Cat# 8820S Nucleoprotein Extraction Kit,Sangon Biotech,Cat# C500009 Cell lines,Cell lines,Cell lines HEK293T

(15) Synaptopodin stress fiber and contractomere at the epithelial junctionThe Journal of cell biologyMay 2, 2022Morris T, Sue E, Geniesse C, Brieher WM, Tang VW.the FRET signal. Active rho detection Rho activity was assayed using the Active Rho Detection Kit (#8820; Cell Signaling Technology). Briefly, confluent monolayers of MDCK and synaptopodin knockdown cells

(16) A Role of Caveolae in Trabecular Meshwork Mechanosensing and Contractile ToneFrontiers in cell and developmental biologyJanuary 1, 2022De Ieso ML, Kuhn M, Bernatchez P, Elliott MH, Stamer WD.were incubated in 1% FBS DMEM 2 h prior to cell lysis. The Active Rho Detection Kit (catalog number 8820; Cell Signaling) was used to perform the Rho activity assay. Cells were washed once with ice cold PBS

(17) Apolipoprotein (a)/Lipoprotein(a)-Induced Oxidative-Inflammatory α7-nAChR/p38 MAPK/IL-6/RhoA-GTP Signaling Axis and M1 Macrophage Polarization Modulate Inflammation-Associated Development of Coronary Artery SpasmOxidative medicine and cellular longevityJanuary 1, 2022Lin YK, Yeh CT, Kuo KT, Fong IH, Yadav VK, Kounis NG, Hu P, Hung MY.1 : 10,000), purchased from Abcam (Abcam plc., Cambridge, UK), and RhoA (#2117; 1 : 1000), RhoA-GTP (#8820; 1 : 1000), ROCK1 (#4035; 1 : 1000), ROCK2 (#9029; 1 : 1000), t-MBS (#2634; 1 : 1000), and p-MBS (#3040;

(18) Genome-Wide miRNA Expression Profiling of Molecular Subgroups of Peripheral T-cell LymphomaClinical cancer research : an official journal of the American Association for Cancer ResearchNovember 1, 2021Lone W, Bouska A, Sharma S, Amador C, Saumyaranjan M, Herek TA, Heavican TB, Yu J, Lim ST, Ong CK, et al.assay The endogenous levels of GTP-bound (active) Rho was detected using the GTPase pull down kit (CST # 8820) as per the manufacturer’s recommendation. Briefly, spin cups were treated with glutathione resin followed

(19) Statin-mediated disruption of Rho GTPase prenylation and activity inhibits respiratory syncytial virus infectionCommunications biologyOctober 29, 2021Malhi M, Norris MJ, Duan W, Moraes TJ, Maynes JT.RIPA buffer 48 h p.i. and GTP-bound RhoA or Rac1 were detected using the Active Rho Detection Kit (8820) and Active Rac1 Detection Kit (8815) (Cell Signaling Technology) as per the manufacturer’s protocols.

(20) Regulation of local GTP availability controls RAC1 activity and cell invasionNature communicationsOctober 19, 2021Bianchi-Smiraglia A, Wolff DW, Marston DJ, Deng Z, Han Z, Moparthy S, Wombacher RM, Mussell AL, Shen S, Chen J, et al.activity assays were performed using the corresponding Active GTPase Detection Kit (Cell Signaling, #8820; #8819; and #8821, respectively) according to the manufacturer’s recommendations. Invasion assay After

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Active Rho Detection Kit

Active Rho Detection Kit from Cell Signaling Technology

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Cell Signaling Technology

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Active Rho Detection Kit from Cell Signaling Technology

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