Active Cdc42 Detection Kit 8819S from Cell Signaling Technology

Product Specs
ItemActive Cdc42 Detection Kit
CompanyCell Signaling Technology
Price $995.00 Inquire
Catalog Number8819S
Quantity1 Kit
Research AreaCytoskeletal Signaling
Detection TargetCdc42
NCBI Full Gene Namecell division cycle 42
NCBI Gene AliasesCDC42Hs, G25K, TKS
Molecular Weight21
ReactivityH, M
ApplicationsFUNC

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Overactivation of Cdc42 GTPase Impairs the Cytotoxic Function of NK Cells From Old Individuals Towards Senescent FibroblastsAging CellFebruary 8, 2026Albert Kallon Koroma, Karmveer Singh, Yongfang Wang, Linda Krug, Philipp Haas, Meinhard Wlaschek, Vadim Sakk, Tanja Schuster, Daniel Fürst, Hubert Schrezenmeier, et al.Pull Down Assay of Cdc42 in NK Cells The active cdc42 detection kit (Cell Signaling Technology Cat. #8819) was used in the cdc42 pull down assay and was done accordingly as stated in the manufacturer's protocol.

(2) Oleate Activates PLD2 Lipase and GEF Activity by Modulating Membrane Microdomain Dynamics via S-acylationJournal of Lipid ResearchNovember 10, 2025Zhiqiang Guo, Karl-Frédérik Bergeron, Catherine MounierFisher Scientific. Additional reagents and kits were obtained as follows: Cdc42 Activation Assay Kit (#8819, Cell Signaling Technology), Bradford protein assay (Bio-Rad, #5000006), Phalloidin–TRITC (Sigma, #P1951),

(3) PAK2 is necessary for myelination in the peripheral nervous systemBrainMay 3, 2024Bo Hu, Daniel Moiseev, Isabella Schena, Bulat Faezov, Roland Dunbrack, Jonathan Chernoff, Jun Liactive RAC1 detection kit (Cat. No. 8815, Cell Signaling) and active CDC42 detection kit (Cat. No. 8819, Cell Signaling), with samples treated with GTPγS (positive control) and GDP (negative control). The

(4) Efficacy and delivery strategies of the dual Rac/Cdc42 inhibitor MBQ-167 in HER2 overexpressing breast cancerTransl OncolMarch 15, 2024Luis E. Velázquez-Vega, Michael Rivera-Robles, Annelis O. Sánchez-Álvarez, Pablo E. Vivas-Mejía, Miciely Aponte-Reyes, Ailed M. Cruz-Collazo, Nilmary Grafals-Ruiz, Stephanie Dorta-Estremera, Eliud Hernández-O'Farrill, Cornelis P Vlaar, et al.Cell Signaling Technology (Danvers, MA) active Rac1 and active Cdc42 Detection kits (Cat. Numbers #8815 and #8819, respectively). Briefly, after 24 hour treatments, cell lysates were incubated with GST-tagged PAK1

(5) Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infectionNature communicationsJanuary 30, 2023Zhao S, Xu Q, Cui Y, Yao S, Jin S, Zhang Q, Wen Z, Ruan H, Liang X, Chao Y, et al.analysis. Cdc42 activity assay Cdc42 activity assays were performed following the manufacturer’s protocol (CST, #8819). Briefly, cells were seeded in 6-well plate in the density of 4 × 105 cells per well, washed in cold

(6) YAP1 induces invadopodia formation by transcriptionally activating TIAM1 through enhancer in breast cancerOncogeneJuly 29, 2022Jie Shen, Qingwen Huang, Weiyi Jia, Shengjie Feng, Liang Liu, Xiaolan Li, Deding Tao, Daxing Xieassay GTP-bound GTPase pull-down assays were performed using an Active RAC1/CDC42 Detection Kit (Cat. #8815, 8819, CST, USA). After transfection or verteporfin treatment, MCF7 cells were harvested, according to the

(7) SS18-SSX drives CREB activation in synovial sarcomaCellular oncology (Dordrecht)June 1, 2022Cyra M, Schulte M, Berthold R, Heinst L, Jansen EP, Grünewald I, Elges S, Larsson O, Schliemann C, Steinestel K, et al.Cell Signaling Technology). The SS18-SSX fusion protein was visualized using an anti-SS18/SYT antibody (#8819 Santa Cruz Biotechnology) detecting the N-terminus of SS18 (which is retained in the SS18-SSX fusion

(8) CIP4 targeted to recruit GTP-Cdc42 involving in invadopodia formation via NF-κB signaling pathway promotes invasion and metastasis of CRCMolecular therapy oncolyticsMarch 17, 2022Hu Z, Zhu J, Ma Y, Long T, Gao L, Zhong Y, Wang X, Li Z.Antibodies against p65 (cat. no. 8242), p-p65 (cat. no. 3033), and the Active Cdc42 Detection Kit (cat. no. 8819) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against His tag (cat.

(9) Regulation of local GTP availability controls RAC1 activity and cell invasionNature communicationsOctober 19, 2021Bianchi-Smiraglia A, Wolff DW, Marston DJ, Deng Z, Han Z, Moparthy S, Wombacher RM, Mussell AL, Shen S, Chen J, et al.assays were performed using the corresponding Active GTPase Detection Kit (Cell Signaling, #8820; #8819; and #8821, respectively) according to the manufacturer’s recommendations. Invasion assay After trypsinization

(10) Exosomes containing miR‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injuryCNS Neuroscience & TherapeuticsMay 1, 2021He Li et al.pull‐down assay was used to determine the activation of Rac1 and Cdc42 as per the kit directions (8815 and 8819, Cell Signaling Technology). Activated Rac1 or Cdc42 were then eluted with sodium dodecyl sulfate and

(11) NDRG1 regulates Filopodia-induced Colorectal Cancer invasiveness via modulating CDC42 activityInternational journal of biological sciencesJanuary 1, 2021Aikemu B, Shao Y, Yang G, Ma J, Zhang S, Yang X, Hong H, Yesseyeva G, Huang L, Jia H, et al.to detect active CDC42 and RAC1 was carried out as the protocol of Active CDC42 Detection Kit (Cat.8819, Cell Signaling Technology) and Active RAC1 Detection Kit (Cat.8815, Cell Signaling Technology). Immunoprecipitation

(12) Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell MigrationFrontiers in cell and developmental biologyJanuary 1, 2021Shi X, Wen Z, Wang Y, Liu YJ, Shi K, Jiu Y.ImageJ. Small GTPase Activity Assay Active RhoA, Rac1 and Cdc42 Detection Kit (#8820, #8815 and #8819, Cell Signaling, Beverly, MO, United States) were used to measure the activity of these small GTPases.

(13) β1 Integrin regulates convergent extension in mouse notogenesis, ensures notochord integrity and the morphogenesis of vertebrae and intervertebral discsDevelopment (Cambridge, England)November 18, 2020Guo SS, Au TYK, Wynn S, Aszodi A, Chan D, Fässler R, Cheah KSE.doi:10.1242/dev.192724 were tested via the detection kits from Cell Signaling Technologies (#8815 and #8819) and analyzed via western blot. Antibodies used for western blot were: integrin β1 (Millipore, MAB1997, 1:2000),

(14) CCN1 interlinks integrin and hippo pathway to autoregulate tip cell activityeLifeAugust 20, 2019Park MH, Kim AK, Manandhar S, Oh SY, Jang GH, Kang L, Lee DW, Hyeon DY, Lee SH, Lee HE, et al.protocol. Briefly, an equal amount of protein was used for GTPase assay (Active Cdc42 detection kit, 8819, Cell Signalling Technology). GST-PAK1-PBD fusion protein was used to bind the activated form of GTP-bound

(15) Endocytosis of commensal antigens by intestinal epithelial cells regulates mucosal T cell homeostasisScience (New York, N.Y.)March 8, 2019Ladinsky MS, Araujo LP, Zhang X, Veltri J, Galan-Diez M, Soualhi S, Lee C, Irie K, Pinker EY, Narushima S, et al.incubated with 20 μg of GST-Human PAK1-PBD and glutathione agarose beads for one hour (Cell Signaling #8819). GTP-bound CDC42 was precipitated and resolved on a 10% SDS-PAGE gel and transferred to a nitrocellulose

(16) Estrogen receptor alpha drives mTORC1 inhibitor-induced feedback activation of PI3K/AKT in ER+ breast cancerOncotargetFebruary 6, 2018Wei Yang, Gary N. Schwartz, Jonathan D. Marotti, Vivian Chen, Nicole A. Traphagen, Jiang Gui, Todd W. MillerIHC www.impactjournals.com/oncotarget CONFLICTS OF INTEREST The authors declare that they have no competing interests. 8819 Oncotarget REFERENCES 1. Taran T, Sahmoud T, Burris HA 3rd. BOLERO-6: Phase II study of everolimus

(17) Inhibition of Cdc42 is essential for Mig-6 suppression of cell migration induced by EGFOncotargetAugust 2, 2016Jiang X, Niu M, Chen D, Chen J, Cao Y, Li X, Ying H, Bergholz J, Zhang Y, Xiao ZX.detection Active Cdc42 level was detected by using Active Cdc42 Detection Kit (Cell Signaling Technologies, #8819). Briefly, GST-PAK1-PBD fusion proteins were used to bind GTP-bound Cdc42, which was then immunoprecipitated

(18) CDC42 inhibition suppresses progression of incipient intestinal tumorsCancer researchOctober 1, 2014Sakamori R, Yu S, Zhang X, Hoffman A, Sun J, Das S, Vedula P, Li G, Fu J, Walker F, et al.was pulled down using GST-human PAK1-PBD supplied by Cdc42 activation kit (Cell Signaling, Catalog # 8819), and detected by Cdc42 antibody (Cell Signaling, #2466). Experiments were repeated on independent biological

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Active Cdc42 Detection Kit

Active Cdc42 Detection Kit from Cell Signaling Technology

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Cell Signaling Technology

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Active Cdc42 Detection Kit from Cell Signaling Technology

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