Active Ras Detection Kit 8821S from Cell Signaling Technology

Product Specs
ItemActive Ras Detection Kit
CompanyCell Signaling Technology
Price $995.00 Inquire
Catalog Number8821S
Quantity1 Kit
Molecular Weight21
Detection TargetHRas
Research AreaMAP Kinase Signaling
NCBI Full Gene NameHRas proto-oncogene, GTPase
NCBI Gene AliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) KRASG12R-Mutant Pancreatic Cancer Features Limited ERK/MAPK Transcriptional Activity and a Distinctive Tumor MicroenvironmentCancer ResearchApril 15, 2026Rachel A. Burge, Ozgun Le Roux, Olesja Popow, Victoria K. Spadafora, Christabelle Rajesh, Sara J. Adair, Lucas Bialousow, Cailey Murphy, Samaneh Saberi, Silvia G. Vaena, et al.GTP pull-down and signaling assays using the Active Ras Detection Kit (Cell Signaling Technology, #8821) according to the manufacturer’s protocol. Cell lines and cell culture conditions The VMP-654, VMP-366,

(2) ADT-030, a novel PDE10 inhibitor, demonstrates potent antitumor activity in pancreatic ductal adenocarcinomabioRxivFebruary 17, 2026Dhana Sekhar Reddy Reddy Bandi, Ganji Purnachandra Nagaraju, Sujith Sarvesh, Jeremy B. Foote, Adam B. Keeton, Xi Chen, Veronica Ramirez-Alcantara, Thomas Holmes, Mehmet Akce, Anju Singh, et al.(RAS-GTP) levels were measured using the active RAS activation assay kit (Cell Signaling Technology, Cat# 8821). Lysates were prepared from cell lines or tumor tissues from various in vivo experiments by performing

(3) Covalent inhibitor design confers activity against both GDP- and GTP-bound forms of KRAS G12CNature CommunicationsJanuary 31, 2026Matthew L. Condakes, Zhuo Zhang, Derek B. Danahy, Richard R. Moore, Sirish Kaushik Lakkaraju, Xiaoliang Zhuo, Yuka Amako, Robert M. Borzilleri, Srividya B. Balachander, Lisa Chourb, et al.ice-cold PBS. Active RAS was isolated using the Active Ras Detection Kit (Cell Signaling Technology, CST-8821) according to the manufacturer’s instructions. Briefly, cell lysates were prepared and incubated with

(4) KRASG12R-Mutant Pancreatic Cancer Features Limited ERK/MAPK Transcriptional Activity and a Distinctive Tumor MicroenvironmentCancer ResJanuary 13, 2026Rachel A. Burge, Ozgun Le Roux, Olesja Popow, Victoria K. Spadafora, Christabelle Rajesh, Sara J. Adair, Lucas Bialousow, Cailey Murphy, Samaneh Saberi, Silvia G. Vaena, et al.GTP pull-down and signaling assays using the Active Ras Detection Kit (Cell Signaling Technology, #8821) according to the manufacturer’s protocol. Cell lines and cell culture conditions The VMP-654, VMP-366,

(5) Binimetinib and encorafenib for the treatment of advanced solid tumors with non-V600E BRAF mutations: results from the Phase II BEAVER trialNature CommunicationsJanuary 3, 2026April A. N. Rose, Jennifer Maxwell, Emmanuelle Rousselle, Chantel L. Mukonoweshuro, Islam E. Elkholi, Melody Riaud, Marco Biondini, Erica Cianfarano, Isabel Soria-Bretones, Chantal Tobin, et al.of activated RAS-GTPase were determined using the Active Ras Detection Kit (Cell Signaling, cat. no. 8821), as per the protocol. Briefly, cells (HMV-II 1,200,000 cells/dish, 22RV1 2,000,000 cells/dish, FM95

(6) Single-molecule imaging quantifies oncogenic KRAS dynamics for enhanced accuracy of therapeutic efficacy assessmentiScienceSeptember 19, 2025Ryoma Yokoi, Toshiki Mori, Koichiro M. Hirosawa, Ryojiro Kondo, Tomohiko Taguchi, Nobuhisa Matsuhashi, Kenichi G.N. SuzukiCell Line Optimization 4D-Nucleofector X Kit,Lonza,Cat# V4XC-9064 Active Ras Detection kit,Cell Signaling,Cat# 8821 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, SW48,ATCC,Cat#

(7) Identification of Small-Molecule Inhibitors that Block the GTP-Binding Pocket of K-Ras and Other Members of the Ras Superfamily of Small GTPasesMolecular Cancer TherapeuticsSeptember 12, 2025Luca Carta, Rebecca Hutcheson, Carolina L. Bigarella, Sufang Zhang, Simon A. Davis, Michael J. Rudolph, Charles H. Reynolds, Matthias Quick, Theresa M. Williams, Michael Schmertzler, et al.200 μL of lysis/binding/wash buffer from the Active Ras Detection kit (Cell Signaling Technology, #8821) supplemented with phenylmethylsulfonyl fluoride (Sigma). An aliquot of the lysate was reserved for

(8) Combined inhibition of SHP2 overcomes adaptive resistance to type 1 BRAF inhibitors in BRAF V600E-driven high-grade gliomaNeuro-Oncology AdvancesSeptember 3, 2025Abiola A Ayanlaja, Michael Chang, Kriti Lalwani, Maria Ioannou, Jiawan Wang, Shreya Jagtap, Yanbo Yang, Calixto-Hope G Lucas, Jean M Mulcahy-Levy, Robyn D Gartrell, et al.and collected for GTP-bound RAS quantification using the Cell Signaling active RAS detection kit (# 8821) or lysed in NP40 lysis buffer for total protein expression. Protein was quantified using the Pierce BCA protein assay kit

(9) Identification of Small-Molecule Inhibitors that Block the GTP-Binding Pocket of K-Ras and Other Members of the Ras Superfamily of Small GTPasesMol Cancer TherAugust 26, 2025Luca Carta, Rebecca Hutcheson, Carolina L. Bigarella, Sufang Zhang, Simon A. Davis, Michael J. Rudolph, Charles H. Reynolds, Matthias Quick, Theresa M. Williams, Michael Schmertzler, et al.200 μL of lysis/binding/wash buffer from the Active Ras Detection kit (Cell Signaling Technology, #8821) supplemented with phenylmethylsulfonyl fluoride (Sigma). An aliquot of the lysate was reserved for

(10) The RASAL2 variant promotes aberrant RAS signaling and resistance to anti-EGFR therapy in colorectal cancerSci RepAugust 23, 2025Yu-Min Yeh, Yi-Hsuan Hsu, Yi-Ting Huang, Peng-Chan Lin, Ya-Ting Hsu, Pei-Ying Wu, Meng-Ru Shenusing ImageJ analysis software. RAS pulldown assay For RAS pulldown assay, Active RAS Dectection Kit (8821, Cell Signaling Technology, MA, USA).) was used. After stimulation with EGF (100 ng/mL) for the indicated

(11) MT-125 inhibits non-muscle myosin IIA and IIB and prolongs survival in glioblastomaCellAugust 21, 2025Kenchappa RS, Radnai L, Young EJ, Zarco N, Lin L, Dovas A, Meyer CT, Haddock A, Hall A, Toth K, et al.Active-Ras, following the instructions in the Active Ras detection kit (Cell Signaling Technology, cat# 8821). Brain histological analysis: Brains from 4% paraformaldehyde-perfused, GBM-bearing mice were paraffin-embedded.

(12) Genetic and oncogenic features of RASGRF fusionsNPJ Precis OncolJuly 5, 2025Sreya Das, Daniel S. Lenchner, Ellen Jaeger, Lisa Hunihan, Dana F. DeSantis, Stamatina Fragkogianni, Karyn Ronski, Frederick H. Wilsonand subjected to RAS activation assays using the Active RAS Detection Kit (Cell Signaling Technology #8821) according to the manufacturer’s instructions. Confocal microscopy Assayed cDNAs were cloned into

(13) KRAS G12V mutation-selective requirement for ACSS2 in colorectal adenoma formationCell ReportsApril 22, 2025Konstantin Budagyan, Alexa C. Cannon, Adam Chatoff, Dorothy Benton, Alison M. Kurimchak, Daniela Araiza-Olivera, Anastasiia Gerasimova, Nathaniel W. Snyder, James S. Duncan, Cristina Uribe-Alvarez, et al.RS-122-2001 Cholesterol/Cholesterol Ester-Glo assay,Promega,Cat# J3190 Active Ras Detection Kit,Cell Signaling Technology,Cat# 8821 Pierce BCA Protein Assay Kit,Thermo Scientific,Cat# 23225 CellTiter-Glo 2.0,Promega,Cat# G9241 NE-PER

(14) MICAL2 Promotes Pancreatic Cancer Growth and MetastasisCancer ResearchMarch 14, 2025Bharti Garg, Sohini Khan, Asimina S. Courelli, Ponmathi Panneerpandian, Deepa Sheik Pran Babu, Evangeline S. Mose, Kevin Christian Montecillo. Gulay, Shweta Sharma, Divya Sood, Alexander T. Wenzel, et al.done according to the manufacturer’s protocol (Active Ras Detection Kit, Cell Signaling Technology, #8821). Gelatin degradation assay and immunofluorescence The gelatin degradation assay was performed essentially

(15) MICAL2 Promotes Pancreatic Cancer Growth and MetastasisCancer ResMarch 14, 2025Bharti Garg, Sohini Khan, Asimina S. Courelli, Ponmathi Panneerpandian, Deepa Sheik Pran Babu, Evangeline S. Mose, Kevin Christian Montecillo Gulay, Shweta Sharma, Divya Sood, Alexander T. Wenzel, et al.done according to the manufacturer’s protocol (Active Ras Detection Kit, Cell Signaling Technology #8821). Gelatin degradation assay and immunofluorescence The gelatin degradation assay was performed essentially

(16) GABAergic Progenitor Cell Graft Rescues Cognitive Deficits in Fragile X Syndrome MiceAdvanced ScienceJanuary 17, 2025Chen Wang, Jia‐Yu Liu, Li‐Da Su, Xin‐Tai Wang, Yu‐Peng Bian, Zhao‐Xiang Wang, Lu‐Yu Ye, Xin‐Jiang Lu, Lin Zhou, Wei Chen, et al.Antibodies against AKT (9272; RRID: AB_329 827), Ras (67 648; RRID: AB_2 910 195) and active Ras Kit (8821) were from CST (Danvers, MA). 4′,6‐diamidino‐2‐phenylindole (DAPI; P36931; SCR_01 5961) and Alexa Fluor‐conjugated

(17) RAS signaling pathway is essential in regulating PIEZO1‐mediated hepatic iron overload in dehydrated hereditary stomatocytosisAmerican Journal of HematologyJanuary 1, 2025Barbara Eleni Rosato, Vanessa D'Onofrio, Roberta Marra, Antonella Nostroso, Federica Maria Esposito, Anthony Iscaro, Vito Alessandro Lasorsa, Mario Capasso, Achille Iolascon, Roberta Russo, et al.assay Quantification of the active total RAS was carried out by using the Active RAS Detection Kit (Cell Signaling, #8821) following the manufacturers' instructions. Briefly, untreated PIEZO1‐WT and PIEZO1‐KI cells were treated

(18) CXCL12 restricts tumor growth by suppressing the Ras, ERK1/2, c-Myc, and the immune checkpoint PD-L1 pathwaysProceedings of the National Academy of SciencesDecember 24, 2024Yelena Kravtsova-Ivantsiv, Gilad Goldhirsh, Aaron CiechanoverProteome Profiler Mouse XL Cytokine Array Kits were from R&D systems. The active Ras detection kit (8821) was from Cell Signaling. Small molecule CXCR4 receptor agonist NUCC-390 was from MedChemExpress. BALB/cOlaHsd-Foxn1nu,

(19) MEK Inhibitors Lead to PDGFR Pathway Upregulation and Sensitize Tumors to RAF Dimer Inhibitors in NF1-Deficient Malignant Peripheral Nerve Sheath TumorClinical Cancer ResearchNovember 15, 2024Miguel A. Miranda-Román, Cindy J. Lee, Eve Fishinevich, Leili Ran, Amish J. Patel, Juan Yan, Makhzuna N. Khudoynazarova, Sarah Warda, Mohini R. Pachai, Yu Chen, et al.after treatment, and GTP-bound RAS was quantified using an active RAS detection kit (Cell Signaling Technology, #8821) according to the manufacturer's instructions. RNA isolation and qRT-PCR Total RNA was isolated from

(20) Picalm, a novel regulator of GLUT4-trafficking in adipose tissueMolecular MetabolismOctober 1, 2024Jasmin Gaugel, Neele Haacke, Ratika Sehgal, Markus Jähnert, Wenke Jonas, Anne Hoffmann, Matthias Blüher, Adhideb Ghosh, Falko Noé, Christian Wolfrum, et al.phospho-S473 Akt (Cell Signaling Technologies, #4060), phospho-T642 Tbc1d4 (Cell Signaling Technologies, #8821), Gapdh (Cell Signaling Technologies, #2118), and GLUT4 (polyclonal antibody recognizing the C-terminus

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Active Ras Detection Kit

Active Ras Detection Kit from Cell Signaling Technology

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Cell Signaling Technology

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Active Ras Detection Kit from Cell Signaling Technology

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