Description
Principle of the Assay: This assay employs the indirect competitive inhibition enzyme immunoassay technique. The coupling antigen is pre-coated on the micro-well stripes.( A Sulfathiazole conjugate has been pre-coated onto a microplate) Then the Sulfathiazole in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Sulfathiazole antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfathiazole in the sample. This (OD) value is compared to the standard curve and the Sulfathiazole concentration is subsequently obtained