Description
Principle of the assay: human MMP-9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-9 has been precoated onto 96-well plates. Standards (NSO,A20-D707) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-9 amount of sample captured in plate.
Background: The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allowangiogenesis and tumor invasion.2 Gelatinase B, a matrix metalloproteinase that has proteolytic activity againstconnective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture.3 MMP-9 is predominantly expressed in neutrophils,macrophages, and mast cells, rather than in oncogene-positive neoplastic cells.4 The polymorphism of MMP-9 actsas a genetic factor for the development of smoking-induced pulmonary emphysema.5 The standard product used inthis kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme