Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with T-2 toxin antigen. Standards or samples are added to the appropriate microtiter plate wells with T-2 toxin specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated T-2 toxin and T-2 toxin in standards or samples with the T-2 toxin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of T-2 toxin in the standards or samples. The color development is stopped and the intensity of the color is measured.
Background/Introduction: T-2 toxin is mycotoxin produced by several Fusarium species. Food crops as wheat, barely, corn, and their products are mainly contaminated. T-2 toxin is a threat for human health and animal husbandry. T-2 toxin primarily affects the blood, liver, kidneys, pancreas, muscles and function of the lymphocyte. The general symptoms of T-2 toxin poisoning are anorexia, vomiting, diarrhea, growth retardation, reproduction and neurological dysfunction. This kit is a detection product developed based on ELISA technology, with operation time as short as 50 min and a sensitivity of 0.15 ppb, and linear range from 0.15 ppb to 4.05 ppb