Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to ADIPOQ has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated ADIPOQ and incubated. A competitive inhibition reaction is launched between ADIPOQ (Standards or samples) and HRP-conjugated ADIPOQ with the pre-coated antibody specific for ADIPOQ. The more amount of ADIPOQ in samples, the less pre-coated antibody specific for ADIPOQ bound by HRP-conjugated ADIPOQ. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of ADIPOQ in the sample. The color development is stopped and the intensity of the color is measured