Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Protein Kinase B (also known as PKB) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat PKB antibody. PKB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PKB Antibody is added and binds to PKB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PKB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat PKB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm