Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Rho-associated Coiled-coil Containing Protein Kinase 2 (also known as Rock-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Rock-2 antibody. Rock-2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Rock-2 Antibody is added and binds to Rock-2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Rock-2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Rock-2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm