Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Bone Morphogenetic Protein 6 (also known as BMP6) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse BMP6 antibody. BMP6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse BMP6 Antibody is added and binds to BMP6 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BMP6 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse BMP6. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm