Description
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ACTH. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated ACTH. A competitive inhibition reaction is launched between ACTH (Standards or samples) and Biotin-conjugated ACTH with the pre-coated antibody specific for ACTH. The more amount of ACTH in samples, the less antibody bound by Biotin-conjugated ACTH. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of ACTH in the sample. The color development is stopped and the intensity of the color is measured