Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse C-type Natriuretic Peptide (also known as CNP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CNP antibody. CNP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CNP Antibody is added and binds to CNP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CNP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CNP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm