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Proteasome-Glo Trypsin-Like Assay
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Proteasome-Glo Trypsin-Like Assay
Proteasome-Glo Trypsin-Like Assay
Proteasome-Glo Trypsin-Like Assay from Promega
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Citations:
(5)
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Proteasome-Glo Trypsin-Like Assay
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Description
The Proteasome-Glo Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in a purified enzyme preparation
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Proteasome-Glo Trypsin-Like Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G8631
Type
Enzyme
Method
Cell-Based Assay
Sample Type
cultured cells
Quantity
10ml
Detection Target
Trypsin-Like
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (5)
(1)
The deubiquitinase USP5 prevents accumulation of protein aggregates in cardiomyocytes
Science advances
January 24, 2025
Eibach Y, Kreher S, Poetsch MS, Kho AL, Gaertner U, Clemen CS, Schröder R, Guo K, Milting H, Meder B,
et al
.
in CMs isolated from either WT or
Usp5
-KO
mice
using Proteasome-Glo
Assays
(
Promega
:
G8622
for CT-L,
G8632
for T-L, and
G8642
for C-L). In brief, cells were lysed using 0.2% NP-40–supplied TSDG buffer (10 mM
(2)
Inhibition of Ubiquitin-Specific Protease-13 Improves Behavioral Performance in Alpha-Synuclein Expressing Mice
International journal of molecular sciences
July 23, 2022
Liu X, Balaraman K, Lynch CC, Hebron M, Shah PK, Hu S, Stevenson M, Wolf C, Moussa C.
Reagent containing Z-LRR-Glo™ Substrate coupled to luciferin (Proteasome-Glo™Tripsin-like
assay
, Cat No:
G8631
,
Promega Corporation
, Madison, WI, USA) for 30 min in dark. Proteasome-induced substrate cleavage generates
(3)
Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins
The EMBO journal
July 1, 2021
Tian G, Hu C, Yun Y, Yang W, Dubiel W, Cheng Y, Wolf DA.
proteasome activity, 50 μl of each fraction were incubated with proteasome detection reagent (
Promega
,
G8631
).
For immunoblotting
, fractions were mixed with 40 μl 4× SDS sample buffer (200 mM Tris/Cl, pH 6.8,
(4)
Identification of Novel Trypanosoma cruzi Proteasome Inhibitors Using a Luminescence-Based High-Throughput Screening Assay
Antimicrobial Agents and Chemotherapy
September 1, 2019
Filip Zmuda, Lalitha Sastry, Sharon M. Shepherd, Deuan Jones, Alison Scott, Peter D. Craggs, Alvaro Cortes, David W. Gray, Leah S. Torrie, Manu De Rycker
bulletin. Proteasome-Glo assay systems. Instructions for use of products
G8531
,
G8532
,
G8621
,
G8622
,
G8632
,
G8641
and
G8642
. https://www.promega.co.uk/products/small -molecule-
drug-discovery/drug-discovery
-workflow/primary-
screening
/ proteasome_glo-assays/?catNum⫽
G8532
. aac.asm.org
(5)
Upregulation of GSK3β Contributes to Brain Disorders in Elderly REGγ-knockout Mice
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
April 1, 2016
Lv Y, Meng B, Dong H, Jing T, Wu N, Yang Y, Huang L, Moses RE, O'Malley BW, Mei B,
et al
.
Activity
Assay
A commercially available indirect enzyme-based
luminescent assay
was modified (Cat. No.
G8631
with substrate for trypsin-like activity (Z-LRR-aminoluciferin),
Promega
, Madison, WI, USA) to
measure
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Proteasome-Glo Trypsin-Like Assay from Promega
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