Proteasome-Glo Chymotrypsin-Like Assay G8622 from Promega

Product Specs
ItemProteasome-Glo Chymotrypsin-Like Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8622
Detection TargetChymotrypsin-Like
Quantity50ml
Molecular Weight~67kDa
TypeEnzyme
Sample Typecultured cells
MethodCell-Based Assay

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Skeletal muscle myosin heavy chain fragmentation following exercise may be linked to post‐exercise inflammation and remodellingExp PhysiolFebruary 11, 2026Dakota R. Tiede, Diego Bittencourt, J. Max Michel, Daniel L. Plotkin, Madison L. Mattingly, Derick A. Anglin, Christopher G. Vann, Zachary A. Graham, Timothy J. Broderick, Marcas M. Bamman, et al.isolates were assayed using commercially available kits (Promega, Madison, WI, USA; cat. nos G8502 and G8622) according to the manufacturer's instructions and as we have previously reported (Michel et al., 2025,

(2) Targeting Cdc42 Improves Motor Phenotype in Parkinson’s Disease Mice and Reveals Age-Dependent Susceptibility to α-SynucleiniScienceDecember 19, 2025Verena Bopp, Jaehyun LeeBae, Patrick Oeckl, Julia K. Kühlwein, Veselin Grozdanov, Martin Kiechle, Benjamin Mayer, Bettina Möhrle, Hartmut Geiger, Karin M. Danzerand a modified version of a commercially available indirect enzyme-based luminescent assay (Promega, G8621), utilizing a substrate designed for caspase-like activity was employed. The setup was adapted to measure

(3) Acidosis attenuates the hypoxic stabilization of HIF-1α by activating lysosomal degradationJ Cell BiolAugust 4, 2025Bobby White, Zhenyi Wang, Matthew Dean, Johanna Michl, Natalia Nieora, Sarah Flannery, Iolanda Vendrell, Roman Fischer, Alzbeta Hulikova, Pawel Swietachmeasurement Proteasomal activity was assessed using the luminescence-based Proteasome-Glo Chymotrypsin-Like Assay (G8621; Promega), following the manufacturer’s instructions. Briefly, SW1222, C99, HDC111, and HT29 were seeded

(4) LNK/SH2B3 Loss Exacerbates the Development of Myeloproliferative Neoplasms in CBL-deficient MiceStem Cell Reviews and ReportsFebruary 1, 2025Yafei Chen, Shangyu Gong, Juan Tang, Xinying Wang, Yudan Gao, Hanying Yang, Wanze Chen, Hailiang Hu, Wei Tong, Kaosheng Lvwas then measured using a plate-reading luminometer. The luminescent substrate Suc-LLVY-Glo™ (Cat.# G8621, Promega) was employed to detect the chymotrypsin-like activity of proteasomes, while Z-nLPnLD-Glo™

(5) The deubiquitinase USP5 prevents accumulation of protein aggregates in cardiomyocytesScience advancesJanuary 24, 2025Eibach Y, Kreher S, Poetsch MS, Kho AL, Gaertner U, Clemen CS, Schröder R, Guo K, Milting H, Meder B, et al.were measured in CMs isolated from either WT or Usp5-KO mice using Proteasome-Glo Assays (Promega: G8622 for CT-L, G8632 for T-L, and G8642 for C-L). In brief, cells were lysed using 0.2% NP-40–supplied TSDG

(6) Actinomycin D and bortezomib disrupt protein homeostasis in Wilms tumorbioRxivDecember 17, 2024Patricia D.B. Tiburcio, Kenian Chen, Lin Xu, Kenneth S. Chento quantify proteasome enzymatic activity. We used Proteasome-Glo™ Chymotrypsin-like Assay (Promega G8621) and calculated the proteasome enzymatic activity in accordance with manufacturer recommendations. Briefly,

(7) Oncogenic Mutant p53 Sensitizes Non-Small Cell Lung Cancer Cells to Proteasome Inhibition via Oxidative Stress-Dependent Induction of Mitochondrial ApoptosisCancer Res CommunOctober 1, 2024Chougoni KK, Neely V, Ding B, Oduah E, Lam VT, Hu B, Koblinski JE, Windle BE, Palit Deb S, Deb S, et al.reader. 26S proteasome activity was measured using a luminescence-based assay (Promega Proteasome-Glo, G8622) using a CLARIOstar plate reader. Glutathione assay Levels of reduced glutathione (GSH) in cell lysates

(8) Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2eLifeApril 15, 2024Ibtisam Ibtisam, Alexei F KisselevDharmacon,T-2001–03,Transfection reagent for MDA-MB-231 and SUM-149 cells Commercial assay or kit,Proteasome-Glo Assay,Promega,G8622,Assay for Chymotrypsin-like Commercial assay or kit,CellTiter-Glo Assay,Promega,G7572,Assay for Cell

(9) Histone Deacetylase (HDAC) Inhibitor Linker Specificity Determinants are Preserved in a Class of Dual HDAC/Non-Covalent Proteasome InhibitorsBioorganic & Medicinal ChemistryApril 15, 2024Alexandria M. Chan, Ashley Mitchell, Lena Grogan, Paul Shapiro, Steven Fletcherpurified human S20 Proteasome (Boston Biochem Cat#E-360). Enzyme and Suc-LLVY-Glo™ substrate (Promega Cat#G8621) are combined in a well of a microtiter plate and incubated for 10 minutes at room temperature. At the

(10) Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2ElifeApril 15, 2024Ibtisam Ibtisam, Alexei F KisselevDharmacon,T-2001–03,Transfection reagent for MDA-MB-231 and SUM-149 cells Commercial assay or kit,Proteasome-Glo Assay,Promega,G8622,Assay for Chymotrypsin-like Commercial assay or kit,CellTiter-Glo Assay,Promega,G7572,Assay for Cell

(11) Discovery and Validation of Novel LonP1 and Proteasome Inhibitor in IDH1-R132H Malignant Astrocytoma ModelsbioRxiv : the preprint server for biologyApril 17, 2023Douglas C, Lomeli N, Lepe J, Di K, Nandwana NK, Vu T, Pham J, Kenney MC, Das B, Bota DA.Proteasome-Glo™ assay system (Promega, G8531), comprising Proteasome-Glo™ Chymotrypsin-Like Assay (G8621), Proteasome-Glo™ Trypsin-Like Assay (G8621) and Proteasome-Glo™ Caspase-Like Assay (G8621). The assay

(12) Characterization of huntingtin interactomes and their dynamic responses in living cells by proximity proteomicsJournal of neurochemistryFebruary 1, 2023Xu H, Bensalel J, Raju S, Capobianco E, Lu ML, Wei J.Chymotrypsin-like proteasomal activity was measured using the cell-based Proteasome-Glo™ assay kit (Cat. No. G8621, Promega) with the luminogenic specific substrate, Suc-LLVY-aminoluciferin, according to the manufacturer’s

(13) SRT1720 as an SIRT1 activator for alleviating paraquat-induced models of Parkinson's diseaseRedox biologyDecember 1, 2022Chao CC, Huang CL, Cheng JJ, Chiou CT, Lee IJ, Yang YC, Hsu TH, Yei CE, Lin PY, Chen JJ, et al.confocal microscopy. 4.13 Proteasome activity assay The Proteasome-Glo™ Chymotrypsin-Like Assay Kit (G8622; Promega, Madison, WI, USA) was used to measure proteasome activity. Cells were collected and homogenized;

(14) Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteinsThe EMBO journalJuly 1, 2021Tian G, Hu C, Yun Y, Yang W, Dubiel W, Cheng Y, Wolf DA.collected and proteasome activity was determined followed the descriptions provided with the kit (Promega, G8622). Polysome profiling 100 μg/ml cycloheximide (CHX) was added to cells grown in 15 cm dishes 10 min

(15) Identification of Novel Trypanosoma cruzi Proteasome Inhibitors Using a Luminescence-Based High-Throughput Screening AssayAntimicrobial Agents and ChemotherapySeptember 1, 2019Filip Zmuda, Lalitha Sastry, Sharon M. Shepherd, Deuan Jones, Alison Scott, Peter D. Craggs, Alvaro Cortes, David W. Gray, Leah S. Torrie, Manu De RyckerProteasome-Glo 3-substrate system and chymotrypsin-like assay kits (Promega; catalogue no. G8531 and G8622, respectively) were assembled according to the manufacturers’ protocols (29) unless otherwise stated.

(16) MG132, a proteasome inhibitor, enhances LDL uptake in HepG2 cells in vitro by regulating LDLR and PCSK9 expressionActa pharmacologica SinicaAugust 1, 2014Yan H, Ma YL, Gui YZ, Wang SM, Wang XB, Gao F, Wang YP.trypsinized. The chymotrypsin activity was detected using the protocol of the Proteasome-GloTM Assay Systems (G8621, Promega). LDLR mRNA stability detection HepG2 cells were treated with MG132 or a vehicle for 6 h, 5 mg/L

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Proteasome-Glo Chymotrypsin-Like Assay

Proteasome-Glo Chymotrypsin-Like Assay from Promega

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Proteasome-Glo Chymotrypsin-Like Assay from Promega

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