Human matrix extracellular phosphoglycoprotein,MEPE ELISA Kit CSB-E09173h from CUSABIO Technology LLC

Product Specs
ItemHuman matrix extracellular phosphoglycoprotein,MEPE ELISA Kit
CompanyCUSABIO Technology LLC
Price $790.00Supplier Page View Company Product Page
Catalog NumberCSB-E09173h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Namematrix extracellular phosphoglycoprotein
NCBI Gene AliasesOF45
Research AreaSignal Transduction
Assay Time1-5h
Detection Targetmatrix extracellular phosphoglycoprotein
Volume50-100ul
Sensitivity3.9 ng/mL
Sample Typeserum, plasma, tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range15.6 ng/mL-1000 ng/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MEPE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MEPE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEPE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEPE bound in the initial step. The color development is stopped and the intensity of the color is measured.

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