Human CD109 antigen(CD109) ELISA Kit CSB-EL004878HU from CUSABIO Technology LLC

Product Specs
ItemHuman CD109 antigen(CD109) ELISA Kit
CompanyCUSABIO Technology LLC
Price $550.00Supplier Page View Company Product Page
Catalog NumberCSB-EL004878HU
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameCD109 molecule
NCBI Gene AliasesCPAMD7, p180, r150
Research AreaImmunology
Assay Time1-5h
Detection TargetCD109 molecule
Volume50-100ul
Sensitivity23.4 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range94 pg/mL-6000 pg/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD109 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD109 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD109 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD109 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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