Human B-cell differentiation antigen CD72(CD72) ELISA Kit CSB-EL004955HU from CUSABIO Technology LLC

Product Specs
ItemHuman B-cell differentiation antigen CD72(CD72) ELISA Kit
CompanyCUSABIO Technology LLC
Price $695.00Supplier Page View Company Product Page
Catalog NumberCSB-EL004955HU
Quantity96T
Detection Range0.9 ng/mL-60 ng/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, tissue homogenates
Sensitivity0.225 ng/mL
Volume50-100ul
Detection TargetCD72 molecule
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene NameCD72 molecule
NCBI Gene AliasesCD72b, LYB2
Research AreaImmunology

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD72 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD72 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD72 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD72 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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