Human Thrombopoietin,TPO ELISA Kit CSB-E04745h from CUSABIO Technology LLC

Product Specs
ItemHuman Thrombopoietin,TPO ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-E04745h
Quantity96T
ApplicationsELISA
Detection Range6.25 pg/mL-400 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
MethodELISA Kit
Sample Typeserum, plasma
Sensitivity1.56 pg/mL
Volume50-100ul
Detection Targetthrombopoietin
Assay Time1-5h
NCBI Full Gene Namethrombopoietin
NCBI Gene AliasesMGDF, MKCSF, ML, MPLLG, THCYT1, TPO
Research AreaCardiovascular

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for THPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any THPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for THPO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of THPO bound in the initial step. The color development is stopped and the intensity of the color is measured.

Add to Compare List