Human bone morphogenetic protein 15 (BMP-15/GDF-9B)ELISA Kit CSB-E17529h from CUSABIO Technology LLC

Product Specs
ItemHuman bone morphogenetic protein 15 (BMP-15/GDF-9B)ELISA Kit
CompanyCUSABIO Technology LLC
Price $550.00Supplier Page View Company Product Page
Catalog NumberCSB-E17529h
Quantity96T
Detection Range15.6 pg/mL-1000 pg/mL
Absorbance / Excitation450 nm
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Sample Typeserum, plasma, cell culture supernates
Sensitivity3.9 pg/mL
Volume50-100ul
Detection Targetbone morphogenetic protein 15
Assay Time1-5h
ApplicationsELISA
MethodELISA Kit
NCBI Full Gene Namebone morphogenetic protein 15
NCBI Gene AliasesGDF9B, ODG2, POF4
Research AreaCardiovascular

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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