Human Platelet-derived growth factor DD(PDGF-DD) ELISA Kit CSB-EL017711HU from CUSABIO Technology LLC

Product Specs
ItemHuman Platelet-derived growth factor DD(PDGF-DD) ELISA Kit
CompanyCUSABIO Technology LLC
Price $550.00Supplier Page View Company Product Page
Catalog NumberCSB-EL017711HU
ApplicationsELISA
MethodELISA Kit
Research AreaOthers
Assay Time1-5h
Detection Targetplatelet derived growth factor DD
Volume50-100ul
Sensitivity0.078 ng/mL
Sample Typeserum, plasma,tissue homogenates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range0.312 ng/mL-20 ng/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGFD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGFD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFD bound in the initial step. The color development is stopped and the intensity of the color is measured.

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