Human Fibroblast growth factor 17(FGF17) ELISA Kit CSB-EL008622HU from CUSABIO Technology LLC

Product Specs
ItemHuman Fibroblast growth factor 17(FGF17) ELISA Kit
CompanyCUSABIO Technology LLC
Price $695.00Supplier Page View Company Product Page
Catalog NumberCSB-EL008622HU
ApplicationsELISA
MethodELISA Kit
Sample Typeserum, plasma, tissue homogenates, cell culture supernates
Detection Range3.12 pg/ml-200 pg/ml
Sensitivity0.78 pg/ml
Research AreaSignal Transduction
NCBI Gene AliasesFGF-13, FGF-17, HH20
NCBI Full Gene Namefibroblast growth factor 17
Assay Time1-5h
Volume50-100ul
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF17 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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