Human Annexin Ⅲ(ANX-Ⅲ) ELISA Kit CSB-E12157h from CUSABIO Technology LLC

Product Specs
ItemHuman Annexin Ⅲ(ANX-Ⅲ) ELISA Kit
CompanyCUSABIO Technology LLC
Price $595.00Supplier Page View Company Product Page
Catalog NumberCSB-E12157h
ApplicationsELISA
MethodELISA Kit
Research AreaSignal Transduction
NCBI Gene AliasesANX3
NCBI Full Gene Nameannexin A3
Assay Time1-5h
Detection Targetannexin A3
Volume50-100ul
Sensitivity0.195 ng/mL
Sample Typeserum, plasma, cell culture supernates, tissue homogenates, cell lysates
PrecisionIntra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Absorbance / Excitation450 nm
Detection Range0.78 ng/mL-50 ng/mL
Quantity96T

Specifications/Features

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANXA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANXA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANXA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANXA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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